DNA-magnetic bead detection using disposable cards and the anisotropic magnetoresistive sensor

A disposable card incorporating specific DNA probes targeting the 16 S rRNA gene of Streptococcus suis was developed for magnetically labeled target DNA detection. A single-stranded target DNA was hybridized with the DNA probe on the SPA/APTES/PDMS/Si as-prepared card, which was subsequently magnetically labeled with superparamagnetic beads for detection using an anisotropic magnetoresistive (AMR) sensor. An almost linear response between the output signal of the AMR sensor and amount of single-stranded target DNA varied from 4.5 to 18 pmol was identified. From the sensor output signal response towards the mass of magnetic beads which were directly immobilized on the disposable card surface, the limit of detection was estimated about 312 ng ferrites, which corresponds to 3.8 μemu. In comparison with DNA detection by conventional biosensor based on magnetic bead labeling, disposable cards are featured with higher efficiency and performances, ease of use and less running cost with respects to consumables for biosensor in biomedical analysis systems operating with immobilized bioreceptor.

[1]  Sara Tombelli,et al.  Biosensors for biomarkers in medical diagnostics , 2008 .

[2]  Depth limitations for in vivo magnetic nanoparticle detection with a compact handheld device , 2015 .

[3]  Jean-Louis Marty,et al.  Disposable Screen Printed Electrochemical Sensors: Tools for Environmental Monitoring , 2014, Sensors.

[4]  Sanjiv S Gambhir,et al.  Protein-functionalized synthetic antiferromagnetic nanoparticles for biomolecule detection and magnetic manipulation. , 2009, Angewandte Chemie.

[5]  Pascal Picart,et al.  Technological conception of an optical biosensor with a disposable card for use with bioluminescent bacteria , 2007 .

[6]  Jian Yin,et al.  Surface Wrinkling on Polydimethylsiloxane Microspheres via Wet Surface Chemical Oxidation , 2014, Scientific Reports.

[7]  J. Genzer,et al.  Surface modification of Sylgard-184 poly(dimethyl siloxane) networks by ultraviolet and ultraviolet/ozone treatment. , 2002, Journal of colloid and interface science.

[8]  Guillaume Lamour,et al.  Contact Angle Measurements Using a Simplified Experimental Setup , 2010 .

[9]  Barbara H. Stuart,et al.  Infrared Spectroscopy: Fundamentals and Applications: Stuart/Infrared Spectroscopy: Fundamentals and Applications , 2005 .

[10]  A. Dillon,et al.  FTIR Studies of H2O and D2O Decomposition on Porous Silicon Surfaces , 1991 .

[11]  Jan Genzer,et al.  Effect of ultraviolet/ozone treatment on the surface and bulk properties of poly(dimethyl siloxane) and poly(vinylmethyl siloxane) networks , 2014 .

[12]  S. Daniel,et al.  Effect of roughness geometry on wetting and dewetting of rough PDMS surfaces. , 2014, Langmuir : the ACS journal of surfaces and colloids.

[13]  Elena Korotkaya,et al.  Biosensors: design, classification, and applications in the food industry , 2014 .

[14]  M. Eberlin,et al.  Efficient green synthesis of bis(cyclic carbonate)poly(dimethylsiloxane) derivative using CO2 addition: a novel precursor for synthesis of urethanes , 2014 .

[15]  D. T. Huong Giang,et al.  Detection of magnetic nanoparticles using simple AMR sensors in Wheatstone bridge , 2016 .

[16]  Yi Wang,et al.  Magnetoresistive performance and comparison of supermagnetic nanoparticles on giant magnetoresistive sensor-based detection system , 2014, Scientific Reports.

[17]  Anna Zhu,et al.  Recent Advances in Optical Biosensors for Environmental Monitoring and Early Warning , 2013, Sensors.

[18]  B. Stuart.  Infrared Spectroscopy , 2004, Analytical Techniques in Forensic Science.

[19]  Maria Strømme,et al.  Sensitive molecular diagnostics using volume-amplified magnetic nanobeads. , 2008, Nano letters.