Coregistration of Small Animal PET and Autoradiography for in vivo-ex vivo Comparison

Non-invasive imaging of small animal and its quantification techniques are needed to be evaluated by comparison with ex vivo image. To overcome the existing method, hand-operated comparison with the unaided eye observation, we present an algorithm that matches the target area in PET scanned in vivo with an autoradiography image acquired ex vivo. We applied a coregistration algorithm that uses voxel similarity to find the corresponding slices and to make quantitative measurements. Automatic matching of in vivo and ex vivo images is novel, and can provide better validation than manual matching techniques.

[1]  Keh-Shih Chuang,et al.  A three-dimensional registration method for automated fusion of micro PET-CT-SPECT whole-body images , 2005, IEEE Transactions on Medical Imaging.

[2]  Dinggang Shen,et al.  Coregistration of Magnetic Resonance and Single Photon Emission Computed Tomography Images for Noninvasive Localization of Stem Cells Grafted in the Infarcted Rat Myocardium , 2006, Molecular Imaging and Biology.

[3]  D. Hill,et al.  Medical image registration , 2001, Physics in medicine and biology.

[4]  Ryan Park,et al.  microPET and autoradiographic imaging of GRP receptor expression with 64Cu-DOTA-[Lys3]bombesin in human prostate adenocarcinoma xenografts. , 2004, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[5]  J. Mazziotta,et al.  MRI‐PET Registration with Automated Algorithm , 1993, Journal of computer assisted tomography.

[6]  Max A. Viergever,et al.  Mutual-information-based registration of medical images: a survey , 2003, IEEE Transactions on Medical Imaging.

[7]  Patrick L Chow,et al.  A method of image registration for small animal, multi-modality imaging , 2006, Physics in medicine and biology.

[8]  Abraham Z Snyder,et al.  Registration of [18F]FDG microPET and small-animal MRI. , 2005, Nuclear medicine and biology.

[9]  Colin Studholme,et al.  An overlap invariant entropy measure of 3D medical image alignment , 1999, Pattern Recognit..

[10]  Max A. Viergever,et al.  Interpolation Artefacts in Mutual Information-Based Image Registration , 2000, Comput. Vis. Image Underst..