Ethyl lauroyl arginate: An update on the antimicrobial potential and application in the food systems: a review

Lauric arginate ethyl ester (LAE), a cationic surfactant with low toxicity, displays excellent antimicrobial activity against a broad range of microorganisms. LAE has been approved as generally recognized as safe (GRAS) for widespread application in certain foods at a maximum concentration of 200 ppm. In this context, extensive research has been carried out on the application of LAE in food preservation for improving the microbiological safety and quality characteristics of various food products. This study aims to present a general review of recent research progress on the antimicrobial efficacy of LAE and its application in the food industry. It covers the physicochemical properties, antimicrobial efficacy of LAE, and the underlying mechanism of its action. This review also summarizes the application of LAE in various foods products as well as its influence on the nutritional and sensory properties of such foods. Additionally, the main factors influencing the antimicrobial efficacy of LAE are reviewed in this work, and combination strategies are provided to enhance the antimicrobial potency of LAE. Finally, the concluding remarks and possible recommendations for the future research are also presented in this review. In summary, LAE has the great potential application in the food industry. Overall, the present review intends to improve the application of LAE in food preservation.

[1]  Qisen Xiang,et al.  Effects of lauroyl arginate ethyl (LAE) on pathogen inactivation and quality attributes of spinach leaves , 2022, Journal of Food Measurement and Characterization.

[2]  Qisen Xiang,et al.  Insight into the antibacterial activity of lauric arginate against Escherichia coli O157:H7: Membrane disruption and oxidative stress , 2022, LWT.

[3]  Y. Liu,et al.  Development of electrospun films enriched with ethyl lauroyl arginate as novel antimicrobial food packaging materials for fresh strawberry preservation , 2021 .

[4]  P. Warszyński,et al.  Ethyl Lauroyl Arginate, an Inherently Multicomponent Surfactant System , 2021, Molecules.

[5]  Mei-Jun Zhu,et al.  Synergistic Effects of Lauric Arginate and Peracetic Acid in Reducing Listeria monocytogenes on Fresh Apples , 2021, Frontiers in Microbiology.

[6]  H. Abdel-Naeem,et al.  Effect of chitosan and lauric arginate edible coating on bacteriological quality, deterioration criteria, and sensory attributes of frozen stored chicken meat , 2021 .

[7]  Ö. Özdestan Ocak,et al.  The effects of ethyl lauroyl arginate and lemon essential oil added edible chitosan film coating on biogenic amines formation during storage in mackerel fillets , 2021 .

[8]  S. Stella,et al.  Ethyl Lauroyl Arginate (LAE): Antimicrobial Activity of LAE-Coated Film for the Packaging of Raw Beef and Pork , 2021, Journal of Food Quality.

[9]  Miguel A. Caballero,et al.  Evaluation of New Antimicrobial Materials Incorporating Ethyl Lauroyl Arginate or Silver into Different Matrices, and Their Safety in Use as Potential Packaging , 2021, Polymers.

[10]  Regiane Ribeiro-Santos,et al.  Nα-lauroyl-l-arginine ethyl ester monohydrochloride, an antimicrobial agent and its use: a review , 2020 .

[11]  Chase E. Golden,et al.  The effect of lauric arginate on the thermal inactivation of starved Listeria monocytogenes in sous-vide cooked ground beef. , 2020, Food research international.

[12]  Yongkang Luo,et al.  Effects of ethyl lauroyl arginate hydrochloride on microbiota, quality and biochemical changes of container-cultured largemouth bass (Micropterus salmonides) fillets during storage at 4 °C. , 2020, Food chemistry.

[13]  D. Mcclements,et al.  Influence of Protein Type on the Antimicrobial Activity of LAE Alone or in Combination with Methylparaben , 2020, Foods.

[14]  M. Karwowska,et al.  Nitrates/Nitrites in Food—Risk for Nitrosative Stress and Benefits , 2020, Antioxidants.

[15]  C. Cutter,et al.  Development and evaluation of pullulan-based composite antimicrobial films (CAF) incorporated with nisin, thymol and lauric arginate to reduce foodborne pathogens associated with muscle foods. , 2020, International journal of food microbiology.

[16]  Tian Ding,et al.  Microbial response to some nonthermal physical technologies , 2020 .

[17]  C. Hill,et al.  Overcoming barriers to phage application in food and feed. , 2019, Current opinion in biotechnology.

[18]  Q. Zhong,et al.  Properties and potential food applications of lauric arginate as a cationic antimicrobial. , 2019, International journal of food microbiology.

[19]  R. Gavara,et al.  Antimicrobial packaging based on a LAE containing zein coating to control foodborne pathogens in chicken soup. , 2019, International journal of food microbiology.

[20]  Rovshen Ishangulyyev,et al.  Understanding Food Loss and Waste—Why Are We Losing and Wasting Food? , 2019, Foods.

[21]  N. Nitin,et al.  Synergistic Antimicrobial Activity by Light or Thermal Treatment and Lauric Arginate: Membrane Damage and Oxidative Stress , 2019, Applied and Environmental Microbiology.

[22]  F. Ghiasi,et al.  The Association between the Preservative Agents in Foods and the Risk of Breast Cancer , 2019, Nutrition and cancer.

[23]  T. Husøy,et al.  Safety of ethyl lauroyl arginate (E 243) as a food additive in the light of the new information provided and the proposed extension of use , 2019, EFSA journal. European Food Safety Authority.

[24]  J. Popp,et al.  Effect of ethyl‐lauroyl‐arginate hypochloride in combination with high hydrostatic pressure processing on the microbial load and physico‐chemical characteristics of minced and portioned chicken breast meat , 2019, Poultry science.

[25]  J. Kerry,et al.  Starch-gelatin antimicrobial packaging materials to extend the shelf life of chicken breast fillets , 2018, LWT.

[26]  A. Rickard,et al.  Combinatorial effect of magnolia bark extract and ethyl lauroyl arginate against multi-species oral biofilms: Food additives with the potential to prevent biofilm-related oral diseases , 2018, Journal of Functional Foods.

[27]  Feng-qin Feng,et al.  Mechanisms of Nα-lauroyl arginate ethyl ester against Penicillium digitatum and Pectobacterium carotovorum subsp. carotovorum , 2018, Journal of Food Science and Technology.

[28]  Feng-qin Feng,et al.  Electrospun Chitosan/Poly(ethylene oxide)/Lauric Arginate Nanofibrous Film with Enhanced Antimicrobial Activity. , 2018, Journal of agricultural and food chemistry.

[29]  Ramón Garduño-Juárez,et al.  Molecular dynamics simulation of the membrane binding and disruption mechanisms by antimicrobial scorpion venom-derived peptides , 2018, Journal of biomolecular structure & dynamics.

[30]  F. Lombó,et al.  Biofilms in the Food Industry: Health Aspects and Control Methods , 2018, Front. Microbiol..

[31]  N. Heredia,et al.  Animals as sources of food-borne pathogens: A review , 2018, Animal nutrition.

[32]  O. Rojas,et al.  Formulation and Stabilization of Concentrated Edible Oil-in-Water Emulsions Based on Electrostatic Complexes of a Food-Grade Cationic Surfactant (Ethyl Lauroyl Arginate) and Cellulose Nanocrystals , 2018, Biomacromolecules.

[33]  D. D’Amico,et al.  Control of Listeria monocytogenes in whole milk using antimicrobials applied individually and in combination. , 2017, Journal of dairy science.

[34]  S. Muñoz-Guerra,et al.  Antibacterial Films Made of Ionic Complexes of Poly(γ-glutamic acid) and Ethyl Lauroyl Arginate , 2017, Polymers.

[35]  Y. Yao,et al.  Biofilm of Escherichia coli O157:H7 on cantaloupe surface is resistant to lauroyl arginate ethyl and sodium hypochlorite. , 2017, International journal of food microbiology.

[36]  Lorena Atarés,et al.  Active starch-gelatin films for shelf-life extension of marinated salmon , 2017 .

[37]  Hyung-Suk Kim,et al.  Evaluation of a novel antimicrobial (lauric arginate ester) substance against biofilm of Escherichia coli O157: H7, Listeria monocytogenes, and Salmonella spp. , 2017 .

[38]  N. Nitin,et al.  Enhanced removal of Escherichia coli O157:H7 and Listeria innocua from fresh lettuce leaves using surfactants during simulated washing , 2017 .

[39]  S. Ha,et al.  Treatment with lauric arginate ethyl ester and commercial bacteriophage, alone or in combination, inhibits Listeria monocytogenes in chicken breast tissue , 2017 .

[40]  D. D’Amico,et al.  Synergistic Antimicrobial Combinations Inhibit and Inactivate Listeria monocytogenes in Neutral and Acidic Broth Systems. , 2017, Journal of food protection.

[41]  R. Nannapaneni,et al.  Evaluation of USDA approved antimicrobials on the reduction of Salmonella and Campylobacter in ground chicken frames and their effect on meat quality , 2017, Poultry science.

[42]  T. Bintsis Foodborne pathogens , 2017, Food Microbiology and Food Safety.

[43]  Y. Yao,et al.  Pathogen biofilm formation on cantaloupe surface and its impact on the antibacterial effect of lauroyl arginate ethyl. , 2017, Food microbiology.

[44]  J. Weiss,et al.  Influence of application sequence and timing of eugenol and lauric arginate (LAE) on survival of spoilage organisms. , 2017, Food microbiology.

[45]  Youngjoo Byun,et al.  Lauroyl Arginate Ethyl Blocks the Iron Signals Necessary for Pseudomonas aeruginosa Biofilm Development , 2017, Front. Microbiol..

[46]  R. Carle,et al.  Effects of Quillaja saponaria extract and Nα-lauroyl-l-arginine ethyl ester on reducing selected foodborne pathogens in vitro and maintaining quality of fresh-cut endive (Cichorium endivia L.) at pilot plant scale , 2017 .

[47]  H. Schmidt,et al.  Antimicrobial effect of lauroyl arginate ethyl on Escherichia coli O157:H7 and Listeria monocytogenes on red oak leaf lettuce , 2017, European Food Research and Technology.

[48]  Q. Zhong,et al.  Quality attributes and microbial survival on whole cantaloupes with antimicrobial coatings containing chitosan, lauric arginate, cinnamon oil and ethylenediaminetetraacetic acid. , 2016, International journal of food microbiology.

[49]  Q. Zhong,et al.  Antimicrobial activities of lauric arginate and cinnamon oil combination against foodborne pathogens: Improvement by ethylenediaminetetraacetate and possible mechanisms , 2016 .

[50]  Samuel I. Miller Antibiotic Resistance and Regulation of the Gram-Negative Bacterial Outer Membrane Barrier by Host Innate Immune Molecules , 2016, mBio.

[51]  Q. Zhong,et al.  Nanoemulsions of thymol and eugenol co-emulsified by lauric arginate and lecithin. , 2016, Food chemistry.

[52]  M. Beltrán,et al.  Assessment of antimicrobial activity of Nα -lauroyl arginate ethylester (LAE®) against Yersinia enterocolitica and Lactobacillus plantarum by flow cytometry and transmission electron microscopy , 2016 .

[53]  S. Parveen,et al.  Application of Antimicrobial Agents via Commercial Spray Cabinet To Inactivate Salmonella on Skinless Chicken Meat. , 2016, Journal of food protection.

[54]  R. Gavara,et al.  Ethyl Lauroyl Arginate (LAE) , 2016 .

[55]  J. Weiss,et al.  Influence of fat addition on the antimicrobial activity of sodium lactate, lauric arginate and methylparaben in minced meat. , 2015, International journal of food microbiology.

[56]  T. Taylor,et al.  Inhibition of Bacterial Pathogens in Medium and on Spinach Leaf Surfaces using Plant-Derived Antimicrobials Loaded in Surfactant Micelles. , 2015, Journal of food science.

[57]  R. Nannapaneni,et al.  Reduction of Salmonella on chicken meat and chicken skin by combined or sequential application of lytic bacteriophage with chemical antimicrobials. , 2015, International journal of food microbiology.

[58]  A. H-Kittikun,et al.  Incorporation of nisin Z and lauric arginate into pullulan films to inhibit foodborne pathogens associated with fresh and ready-to-eat muscle foods. , 2015, International journal of food microbiology.

[59]  P. Davidson,et al.  Antimicrobial Activity of Cinnamaldehyde, Carvacrol, and Lauric Arginate against Salmonella Tennessee in a Glycerol-Sucrose Model and Peanut Paste at Different Fat Concentrations. , 2015, Journal of food protection.

[60]  L. Harris,et al.  Evaluation of microbial loads and the effects of antimicrobial sprays in postharvest handling of California walnuts. , 2015, Food microbiology.

[61]  D. Mcclements,et al.  Fabrication, stability and efficacy of dual-component antimicrobial nanoemulsions: essential oil (thyme oil) and cationic surfactant (lauric arginate). , 2015, Food chemistry.

[62]  A. H. Brown,et al.  Single or multiple decontamination interventions involving lauric arginate on beef trimmings to enhance microbial safety of ground beef , 2014 .

[63]  A. H-Kittikun,et al.  Effect of lauric arginate, nisin Z, and a combination against several food-related bacteria. , 2014, International journal of food microbiology.

[64]  R. Nannapaneni,et al.  Reduction of Listeria monocytogenes in cold-smoked salmon by bacteriophage P100, nisin and lauric arginate, singly or in combinations , 2014 .

[65]  C. Nerín,et al.  Evaluation of two antimicrobial packaging films against Escherichia coli O157:H7 strains in vitro and during storage of a Spanish ripened sheep cheese (Zamorano) , 2014 .

[66]  D. Mcclements,et al.  Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts , 2014, Journal of applied microbiology.

[67]  J. Sebranek,et al.  Investigating the control of Listeria monocytogenes on a ready-to-eat ham product using natural antimicrobial ingredients and postlethality interventions. , 2014, Foodborne pathogens and disease.

[68]  M. Guo,et al.  Antimicrobial films and coatings for inactivation of Listeria innocua on ready-to-eat deli turkey meat , 2014 .

[69]  S. Ricke,et al.  Temperature effects on the antimicrobial efficacy of condensed smoke and lauric arginate against Listeria and Salmonella. , 2014, Journal of food protection.

[70]  ZhengZuoxing Ingredient Technology for Food Preservation , 2014 .

[71]  R. Nannapaneni,et al.  Antimicrobial efficacy of lauric arginate against Campylobacter jejuni and spoilage organisms on chicken breast fillets. , 2013, Poultry science.

[72]  R. Gavara,et al.  Antimicrobial Effectiveness of Lauroyl Arginate Incorporated into Ethylene Vinyl Alcohol Copolymers to Extend the Shelf-Life of Chicken Stock and Surimi Sticks , 2014, Food and Bioprocess Technology.

[73]  Q. Zhong,et al.  Antimicrobial properties of lauric arginate alone or in combination with essential oils in tryptic soy broth and 2% reduced fat milk. , 2013, International journal of food microbiology.

[74]  C. Nerín,et al.  Antimicrobial activity of Lauroyl Arginate Ethyl (LAE), against selected food-borne bacteria , 2013 .

[75]  R. Gavara,et al.  Development of a novel antimicrobial film based on chitosan with LAE (ethyl-N(α)-dodecanoyl-l-arginate) and its application to fresh chicken. , 2013, International journal of food microbiology.

[76]  D. Fung,et al.  Efficacy of Antimicrobial Lauric Arginate against Listeria monocytogenes on Stainless Steel Coupons , 2013 .

[77]  Sadia Seemeen EFFECTS OF HIGH PRESSURE PROCESSING AND ETHYL LAUROYL ARGINATE ON THE SHELF-LIFE OF READY-TO-EAT SLICED CHICKEN BREAST ROAST , 2013 .

[78]  Suchart Suksathit,et al.  Activity of organic acid salts in combination with lauric arginate against Listeria monocytogenes and Salmonella Rissen , 2013 .

[79]  R. Nannapaneni,et al.  Reduction of Listeria monocytogenes in queso fresco cheese by a combination of listericidal and listeriostatic GRAS antimicrobials. , 2012, International journal of food microbiology.

[80]  W. Visessanguan,et al.  Antimicrobial activity of lauric arginate-coated polylactic acid films against Listeria monocytogenes and Salmonella typhimurium on cooked sliced ham. , 2012, Journal of food science.

[81]  D. Mcclements,et al.  Influence of surfactant charge on antimicrobial efficacy of surfactant-stabilized thyme oil nanoemulsions. , 2011, Journal of agricultural and food chemistry.

[82]  J. T. Keeton,et al.  Efficacy of ε-polylysine, lauric arginate, or acidic calcium sulfate applied sequentially for Salmonella reduction on membrane filters and chicken carcasses. , 2011, Journal of food protection.

[83]  R. Nannapaneni,et al.  Bactericidal activity of lauric arginate in milk and Queso Fresco cheese against Listeria monocytogenes cold growth. , 2010, Journal of dairy science.

[84]  J. Call,et al.  Control of Listeria monocytogenes on commercially-produced frankfurters prepared with and without potassium lactate and sodium diacetate and surface treated with lauric arginate using the Sprayed Lethality in Container (SLIC(R)) delivery method. , 2010, Meat science.

[85]  D. Swerdlow,et al.  Epidemiology of Seafood-Associated Infections in the United States , 2010, Clinical Microbiology Reviews.

[86]  D. Visser,et al.  Control of Listeria monocytogenes on cooked cured ham by formulation with a lactate-diacetate blend and surface treatment with lauric arginate. , 2010, Journal of Food Protection.

[87]  P. Taormina,et al.  Inactivation of Listeria monocytogenes on hams shortly after vacuum packaging by spray application of lauric arginate. , 2009, Journal of food protection.

[88]  D. R. Hawkins,et al.  Metabolism and pharmacokinetics of ethyl N(alpha)-lauroyl-L-arginate hydrochloride in human volunteers. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[89]  K. Boor,et al.  Short communication: Nalpha-lauroyl-L-arginine ethylester monohydrochloride reduces bacterial growth in pasteurized milk. , 2009, Journal of dairy science.

[90]  S. Ricke,et al.  Control of Listeria monocytogenes by lauric arginate on frankfurters formulated with or without lactate/diacetate. , 2009, Journal of food science.

[91]  P. Taormina,et al.  Short-term bactericidal efficacy of lauric arginate against Listeria monocytogenes present on the surface of frankfurters. , 2009, Journal of food protection.

[92]  D. Mcclements,et al.  Analysis of the interactions of a cationic surfactant (lauric arginate) with an anionic biopolymer (pectin): isothermal titration calorimetry, light scattering, and microelectrophoresis. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[93]  S. Condón,et al.  Resistance of Escherichia coli grown at different temperatures to various environmental stresses , 2008, Journal of applied microbiology.

[94]  Flavourings Opinion of the Scientific Panel on food additives, flavourings, processing aids and materials in contact with food (AFC) related to an application on the use of ethyl lauroyl arginate as a food additive , 2007 .

[95]  J. Call,et al.  Viability of Listeria monocytogenes on commercially-prepared hams surface treated with acidic calcium sulfate and lauric arginate and stored at 4°C. , 2005, Meat science.

[96]  E. Rodríguez,et al.  Cellular effects of monohydrochloride of l‐arginine, Nα‐lauroyl ethylester (LAE) on exposure to Salmonella typhimurium and Staphylococcus aureus , 2004, Journal of applied microbiology.

[97]  Chad B. Sandusky,et al.  Toxicological and metabolic investigations of the safety of N-alpha-lauroyl-L-arginine ethyl ester monohydrochloride (LAE). , 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[98]  J. G. Domínguez,et al.  Surface active molecules: preparation and properties of long chain nα‐acyl‐l‐α‐amino‐ω‐guanidine alkyl acid derivatives , 1984, International journal of cosmetic science.