Imaging Flow Cytometry and High-Throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi

Traditional methods for automated and high-throughput morphological analysis of filamentous fungi can be time-consuming and difficult. Here, two suitable image acquisition methods and subsequent image and data processing steps are presented. The acquisition methods are based on imaging flow cytometry and whole-slide microscopy. Guidelines for developing customized image and data analysis routines for satisfying individual research requirements are presented.