Electrofocusing patterns of fucosyltransferase activity in plasma of patients with chronic granulocytic leukemia.

Fucosyltransferase activity was measured in plasma samples obtained from patients with chronic granulocytic leukemia in different disease stages. Total enzyme activity was generally elevated in untreated and in stable disease; a marked further elevation was noted 30 to 60 days before development of blast crisis. Electrofocusing studies indicate the presence of three major enzymes. The level of enzyme which focused at pH 5.6 was elevated in untreated disease and rose markedly during blastic transformation. A second enzyme focused at pH 5.1 and appeared to be related to marrow erythroid turnover. A third enzyme with pI = 4.7 was elevated only during marrow regeneration which followed myelosuppressive therapy. These data suggest the enzyme with pI = 5.6 to be a specific marker in chronic granulocytic leukemia.

[1]  R. Oliver,et al.  Leukemia-induced alterations of serum glycosyltransferase enzymes. , 1980, Cancer research.

[2]  S. Chatterjee.  Glycosyltransferases in metastasizing and non-metastasizing rat mammary tumors and the release of these enzymes in the host sera. , 1979, European journal of cancer.

[3]  D. Kessel,et al.  Electrofocusing patterns of fucosyltransferases in plasma of patients with neoplastic disease. , 1979, Cancer research.

[4]  D. Kessel,et al.  Delineation of fucosyltransferase activities with thiol reagents. , 1979, The Biochemical journal.

[5]  J. Montreuil,et al.  Chemical and enzymic degradations of nucleoside mono- and diphosphate sugars. I. Determination of the degradation rate during the glycosyltransferase assays. , 1979, Biochimica et biophysica acta.

[6]  R. Hill,et al.  Characterization of Two Highly Purified Fucosyltransferases , 1979 .

[7]  W. Reutter,et al.  Decrease of human serum fucosyltransferase as an indicator of successful tumor therapy. , 1978, Science.

[8]  C. Gahmberg,et al.  IDENTIFICATION AND CHARACTERIZATION OF NORMAL AND MALIGNANT HUMAN BLOOD LEUKOCYTES BY SURFACE GLYCOPROTEIN PATTERNS * , 1978, Annals of the New York Academy of Sciences.

[9]  C. Ip,et al.  Alterations in serum glycosyltransferases and 5'-nucleotidase in breast cancer patients. , 1978, Cancer research.

[10]  D. Kessel,et al.  Evaluation of two plasma fucosyltransferases as marker enzymes in non‐Hodgkin's lymphoma , 1978, Cancer.

[11]  D. Kessel,et al.  Guanosine diphosphate-L-fucose plasma: N-acetylglucosaminide fucosyltransferase as in index of bone marrow hyperplasia after chemotherapy. , 1978, Cancer research.

[12]  D. Kessel,et al.  Levels of two plasma fucosyltransferases as an index of disease status in acute myelogenous leukemias. , 1978, Cancer treatment reports.

[13]  D. Kessel,et al.  Variation of levels of plasma guanosine diphosphate L-fucose:beta-D-galactosyl alpha-2-L-fucosyltransferase in acute adult leukemia. , 1977, Cancer research.

[14]  D. Kessel,et al.  Selective inhibition of a plasma fucosyltransferase by N-ethylmaleimide. , 1977, Biochemical and biophysical research communications.

[15]  M. Chester,et al.  Phenyl beta-D-Galactopyranoside as an Acceptor Substrate for the Blood-Group H Gene-Associated Guanosine Diphosphate L-Fucose:beta-D-Galactosyl alpha-2-L-Fucosyltransferase , 1976 .

[16]  D. Kessel,et al.  Elevated plasma sialyltransferase in the cancer patient. , 1975, Cancer research.

[17]  H. Schenkel-Brunner,et al.  Alpha-L-fucosyltransferases in human serum from donors of different ABO, secretor and Lewis blood-group phenotypes. , 1972, European journal of biochemistry.

[18]  V. Ginsburg.  [51] Formation of GDP-l-fucose from GDP-d-mannose , 1966 .

[19]  R. Spiro.  PERIODATE OXIDATION OF THE GLYCOPROTEIN FETUIN. , 1964, The Journal of biological chemistry.