Standard methods for inexpensive pollen loads authentication by means of computer vision and machine learning

We present a complete methodology for authenticating local bee pollen against fraudulent samples using image processing and machine learning techniques. The proposed standard methods do not need expensive equipment such as advanced microscopes and can be used for a preliminary fast rejection of unknown pollen types. The system is able to rapidly reject the non-local pollen samples with inexpensive hardware and without the need to send the product to the laboratory. Methods are based on the color properties of bee pollen loads images and the use of one-class classifiers which are appropriate to reject unknown pollen samples when there is limited data about them. The validation of the method is carried out by authenticating Spanish bee pollen types. Experimentation shows that the proposed methods can obtain an overall authentication accuracy of 94%. We finally illustrate the user interaction with the software in some practical cases by showing the developed application prototype.

[1]  Ian H. Witten,et al.  Data mining - practical machine learning tools and techniques, Second Edition , 2005, The Morgan Kaufmann series in data management systems.

[2]  J. R. Flenley,et al.  Towards automation of palynology 1: analysis of pollen shape and ornamentation using simple geometric measures, derived from scanning electron microscope images , 2004 .

[3]  A.W.G. Duller,et al.  A new approach to automated pollen analysis , 2000 .

[4]  Dorin Comaniciu,et al.  Mean Shift: A Robust Approach Toward Feature Space Analysis , 2002, IEEE Trans. Pattern Anal. Mach. Intell..

[5]  Barry R. Masters,et al.  Digital Image Processing, Third Edition , 2009 .

[6]  K. R. Markham,et al.  An approach to the characterization of bee pollens via their flavonoid/phenolic profiles , 1997 .

[7]  Mª Pilar de Sá-Otero,et al.  Método de determinación del origen geográfico del polen apícola comercial , 2002 .

[8]  B. Stoel,et al.  Feasibility study on automated recognition of allergenic pollen: grass, birch and mugwort , 2006 .

[9]  J. R. Flenley,et al.  Towards automation of palynology 3: pollen pattern recognition using Gabor transforms and digital moments , 2004 .

[10]  N. Otsu A threshold selection method from gray level histograms , 1979 .

[11]  Belur V. Dasarathy,et al.  Nearest neighbor (NN) norms: NN pattern classification techniques , 1991 .

[12]  Manuel Chica,et al.  Authentication of bee pollen grains in bright‐field microscopy by combining one‐class classification techniques and image processing , 2012, Microscopy research and technique.

[13]  Gunter Ritter,et al.  Outliers in statistical pattern recognition and an application to automatic chromosome classification , 1997, Pattern Recognit. Lett..

[14]  Edward Y. Chang,et al.  Using one-class and two-class SVMs for multiclass image annotation , 2005, IEEE Transactions on Knowledge and Data Engineering.

[15]  José M. Alonso,et al.  HILK++: an interpretability-guided fuzzy modeling methodology for learning readable and comprehensible fuzzy rule-based classifiers , 2011, Soft Comput..

[16]  J. R. Flenley,et al.  Towards automation of palynology 2: the use of texture measures and neural network analysis for automated identification of optical images of pollen grains , 2004 .

[17]  Christopher M. Bishop,et al.  Novelty detection and neural network validation , 1994 .

[18]  K. Arunachalam,et al.  Phytochemical Analysis , 2021, Springer Protocols Handbooks.

[19]  Edward Y. Chang,et al.  SVM binary classifier ensembles for image classification , 2001, CIKM '01.

[20]  Larry D. Hostetler,et al.  The estimation of the gradient of a density function, with applications in pattern recognition , 1975, IEEE Trans. Inf. Theory.

[21]  M. M. Moya,et al.  One-class classifier networks for target recognition applications , 1993 .

[22]  Alexander Dekhtyar,et al.  Information Retrieval , 2018, Lecture Notes in Computer Science.

[23]  Ian H. Witten,et al.  Data mining: practical machine learning tools and techniques, 3rd Edition , 1999 .

[24]  VARUN CHANDOLA,et al.  Anomaly detection: A survey , 2009, CSUR.

[25]  O. Ronneberger,et al.  Automated pollen recognition using 3D volume images from fluorescence microscopy , 2002 .

[26]  Monique Thonnat,et al.  Development of a semi-automatic system for pollen recognition , 2002 .

[27]  Pascual Campoy,et al.  Discernment of bee pollen loads using computer vision and one-class classification techniques , 2012 .

[28]  E. Cernadas,et al.  Automatic detection and classification of grains of pollen based on shape and texture , 2006, IEEE Transactions on Systems, Man, and Cybernetics, Part C (Applications and Reviews).

[29]  Wilhelm Burger,et al.  Digital Image Processing - An Algorithmic Introduction using Java , 2008, Texts in Computer Science.

[30]  Pilar Carrión,et al.  Classification of honeybee pollen using a multiscale texture filtering scheme , 2004, Machine Vision and Applications.

[31]  Johan H C Reiber,et al.  Detection of pollen grains in multifocal optical microscopy images of air samples , 2009, Microscopy research and technique.