Epitope-Blocking Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Ross River Virus in Vertebrate Sera

ABSTRACT We describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) for the sensitive and rapid detection of antibodies to Ross River virus (RRV) in human sera and known vertebrate host species. This ELISA provides an alternative method for the serodiagnosis of RRV infections.

[1]  R. Hall,et al.  Epitope-Blocking Enzyme-Linked Immunosorbent Assays for Detection of West Nile Virus Antibodies in Domestic Mammals , 2003, Journal of Clinical Microbiology.

[2]  J. Roehrig,et al.  Epitope-Blocking Enzyme-Linked Immunosorbent Assays for the Detection of Serum Antibodies to West Nile Virus in Multiple Avian Species , 2003, Journal of Clinical Microbiology.

[3]  F. Birrell,et al.  Ross River virus. , 2001, Australian family physician.

[4]  R. Hall,et al.  The emergence of West Nile virus: the Australian connection. , 2000, Viral immunology.

[5]  M. J. Howard,et al.  Immunodominant epitopes on the NS1 protein of MVE and KUN viruses serve as targets for a blocking ELISA to detect virus-specific antibodies in sentinel animal serum. , 1995, Journal of virological methods.

[6]  J. Aaskov,et al.  Specific and non-specific immunological changes in epidemic polyarthritis patients. , 1981, The Australian journal of experimental biology and medical science.

[7]  J. Aaskov,et al.  An immunofluorescence assay for human antibodies to Ross River virus. , 1979, JIM - Journal of Immunological Methods.

[8]  J. Casals,et al.  Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. , 1958, The American journal of tropical medicine and hygiene.

[9]  B. Kay,et al.  Identification of Australian arboviruses in inoculated cell cultures using monoclonal antibodies in ELISA , 1998, Pathology.

[10]  B. Kay,et al.  Epitope analysis of Ross River virus using monoclonal antibodies , 1997 .

[11]  M. Lindsay Ecology and epidemiology of Ross River virus in Western Australia , 1995 .

[12]  David W Smith,et al.  Diagnosis and reporting of arbovirus infections in Australia , 1992 .

[13]  M. J. Howard,et al.  Specific monitoring of flavivirus infections in man and sentinel animals by competitve ELISA , 1992 .

[14]  B. Kay,et al.  Ecology of Arboviruses and Their Vectors in Australia , 1987 .

[15]  L. Smythe,et al.  DETECTION OF ROSS RIVER VIRUS IMMUNOGLOBULIN M ANTIBODIES BY ENZYME‐LINKED IMMUNOSORBENT ASSAY USING ANTIBODY CLASS CAPTURE AND COMPARISON WITH OTHER METHODS , 1985, Pathology.

[16]  J. Aaskov,et al.  Detection by ELISA of IgM antibodies to Ross River virus in serum from patients with suspected epidemic polyarthritis. , 1983, Bulletin of the World Health Organization.