Novel phenol biosensor based on laccase immobilized on reduced graphene oxide supported palladium-copper alloyed nanocages.

Developing new nanomaterials is of key importance to improve the analytical performances of electrochemical biosensors. In this work, palladium-copper alloyed nanocages supported on reduced graphene oxide (RGO-PdCu NCs) were facilely prepared by a simple one-pot solvothermal method. A novel phenol biosensor based on laccase has been constructed for rapid detection of catachol, using RGO-PdCu NCs as electrode material. The as-developed phenol biosensor greatly enhanced the electrochemical signals for catechol. Under the optimal conditions, the biosensor has two linear ranges from 0.005 to 1.155 mM and 1.655 to 5.155 mM for catachol detection at 0.6 V, the sensitivity of 12.65 µA mM(-1) and 5.51 µA mM(-1), respectively. This biosensor showed high selectivity, low detection limit, good reproducibility, and high anti-interference ability.

[1]  Wenliang Gao,et al.  Titania supported Pd-Cu bimetallic catalyst for the reduction of nitrate in drinking water , 2003 .

[2]  L. Mita,et al.  A thionine-modified carbon paste amperometric biosensor for catechol and bisphenol A determination. , 2010, Biosensors & bioelectronics.

[3]  Petr Skládal,et al.  Amperometric biosensors for detection of phenol using chemically modified electrodes containing immobilized bacteria. , 2002, Biosensors & bioelectronics.

[4]  P. Schmuki,et al.  One-dimensional titanium dioxide nanomaterials: nanotubes. , 2014, Chemical Reviews.

[5]  Jianrong Chen,et al.  One-pot synthesis of monodisperse palladium–copper nanocrystals supported on reduced graphene oxide nanosheets with improved catalytic activity and methanol tolerance for oxygen reduction reaction , 2014 .

[6]  Songjun Li,et al.  Enzyme-free electrochemical immunosensor configured with Au-Pd nanocrystals and N-doped graphene sheets for sensitive detection of AFP. , 2013, Biosensors & bioelectronics.

[7]  Li Wang,et al.  Electrochemical sensing and biosensing platform based on biomass-derived macroporous carbon materials. , 2014, Analytical chemistry.

[8]  Jun Yan,et al.  An environmentally friendly and efficient route for the reduction of graphene oxide by aluminum powder , 2010 .

[9]  X. Bao,et al.  Supported Pd-Cu bimetallic nanoparticles that have high activity for the electrochemical oxidation of methanol. , 2012, Chemistry.

[10]  R. Wightman.  Probing Cellular Chemistry in Biological Systems with Microelectrodes , 2006, Science.

[11]  P. Sahoo,et al.  Facile synthesis of reduced graphene oxide/Pt–Ni nanocatalysts: their magnetic and catalytic properties , 2014 .

[12]  Jun Chen,et al.  Mesoporous hollow PtCu nanoparticles for electrocatalytic oxygen reduction reaction , 2013 .

[13]  I. Oh,et al.  Microwave syntheses of graphene and graphene decorated with metal nanoparticles , 2011 .

[14]  Bernhard Wolfrum,et al.  Nanofluidic redox cycling amplification for the selective detection of catechol. , 2008, Analytical chemistry.

[15]  Li Wang,et al.  One-step synthesis of Pt–NiO nanoplate array/reduced graphene oxide nanocomposites for nonenzymatic glucose sensing , 2015 .

[16]  Nader Sheibani,et al.  Direct electrochemistry of glucose oxidase and glucose biosensing on a hydroxyl fullerenes modified glassy carbon electrode. , 2014, Biosensors & bioelectronics.

[17]  Jianrong Chen,et al.  Monodisperse Au-Pd bimetallic alloyed nanoparticles supported on reduced graphene oxide with enhanced electrocatalytic activity towards oxygen reduction reaction , 2014 .

[18]  A. Castoldi,et al.  Direct Analysis of Phenol, Catechol and Hydroquinone in Human Urine by Coupled-Column HPLC with Fluorimetric Detection , 2005 .

[19]  T. Itoh,et al.  Amperometric detection of phenolic compounds with enzyme immobilized in mesoporous silica prepared by electrophoretic deposition , 2011 .

[20]  F. Shahidi,et al.  Extraction and analysis of phenolics in food. , 2004, Journal of chromatography. A.

[21]  A. Barron,et al.  Ultrasmall copper nanoparticles from a hydrophobically immobilized surfactant template. , 2009, Nano letters.

[22]  Yadong Li,et al.  Bimetallic Pd-Cu nanocrystals and their tunable catalytic properties. , 2014, Chemical Communications.

[23]  S. Stankovich,et al.  Synthesis of graphene-based nanosheets via chemical reduction of exfoliated graphite oxide , 2007 .

[24]  Hui Zhang,et al.  Facile synthesis of Pd-Pt alloy nanocages and their enhanced performance for preferential oxidation of CO in excess hydrogen. , 2011, ACS nano.

[25]  Dawei Li,et al.  Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection , 2014, Sensors.

[26]  Fan Yang,et al.  Ultrasensitive electrochemical immunosensor for carbohydrate antigen 19-9 using Au/porous graphene nanocomposites as platform and Au@Pd core/shell bimetallic functionalized graphene nanocomposites as signal enhancers. , 2015, Biosensors & bioelectronics.

[27]  P. Messersmith,et al.  Catechol Polymers for pH-Responsive, Targeted Drug Delivery to Cancer Cells , 2011, Journal of the American Chemical Society.

[28]  Zhilei Yin,et al.  A facile route to the synthesis copper oxide/reduced graphene oxide nanocomposites and electrochemical detection of catechol organic pollutant , 2012 .

[29]  Yawen Zhang,et al.  Chitosan coated on the layers' glucose oxidase immobilized on cysteamine/Au electrode for use as glucose biosensor. , 2014, Biosensors & bioelectronics.

[30]  Jianrong Chen,et al.  Facile synthesis of porous Pt–Pd nanospheres supported on reduced graphene oxide nanosheets for enhanced methanol electrooxidation , 2014 .

[31]  Aneeya K. Samantara,et al.  A facile approach for in situ synthesis of graphene-branched-Pt hybrid nanostructures with excellent electrochemical performance. , 2013, Nanoscale.

[32]  Wenrong Yang,et al.  Protein electrochemistry using graphene-based nano-assembly: an ultrasensitive electrochemical detection of protein molecules via nanoparticle-electrode collisions. , 2014, Chemical communications.

[33]  Qingji Xie,et al.  Biofuel cell and phenolic biosensor based on acid-resistant laccase-glutaraldehyde functionalized chitosan-multiwalled carbon nanotubes nanocomposite film. , 2009, Biosensors & bioelectronics.

[34]  Jianrong Chen,et al.  Simple synthesis of platinum–palladium nanoflowers on reduced graphene oxide and their enhanced catalytic activity for oxygen reduction reaction , 2014 .

[35]  L. Qu,et al.  Small-sized PdCu nanocapsules on 3D graphene for high-performance ethanol oxidation. , 2014, Nanoscale.

[36]  Lei Ding,et al.  Novel phenolic biosensor based on a magnetic polydopamine-laccase-nickel nanoparticle loaded carbon nanofiber composite. , 2014, ACS applied materials & interfaces.

[37]  J. Barek,et al.  Determination of 2-Nitrophenol, 4-Nitrophenol, 2-Methoxy-5-nitrophenol, and 2,4-Dinitrophenol by Differential Pulse Voltammetry and Adsorptive Stripping Voltammetry , 1994 .

[38]  Xiangheng Niu,et al.  Novel snowflake-like Pt-Pd bimetallic clusters on screen-printed gold nanofilm electrode for H2O2 and glucose sensing. , 2012, Biosensors & bioelectronics.

[39]  V. Faraco,et al.  Laccases: a never-ending story , 2010, Cellular and Molecular Life Sciences.

[40]  Hanchang Shi,et al.  A reduced graphene oxide based biosensor for high-sensitive detection of phenols in water samples , 2013 .

[41]  G. Zeng,et al.  Mesoporous carbon nitride based biosensor for highly sensitive and selective analysis of phenol and catechol in compost bioremediation. , 2014, Biosensors & bioelectronics.

[42]  M. Stoytcheva,et al.  Detection of phenolic compounds by tyrosinase modified clark type electrode , 2014 .

[43]  E. Wang,et al.  Cu nanoclusters with aggregation induced emission enhancement. , 2013, Small.

[44]  Fei Xiao,et al.  One-step electrochemical synthesis of PtNi nanoparticle-graphene nanocomposites for nonenzymatic amperometric glucose detection. , 2011, ACS applied materials & interfaces.

[45]  Bernardo de Souza,et al.  PEI-coated gold nanoparticles decorated with laccase: a new platform for direct electrochemistry of enzymes and biosensing applications. , 2013, Biosensors & bioelectronics.