Nutritional Improvement of Fresh Cheese with Microalga Chlorella vulgaris: Impact on Composition, Structure and Sensory Acceptance

SUMMARY Research background The production of foods fortified with bioactive ingredients has been recognized by food companies as a way to position their products in health food markets. The fortification of cheese represents a major challenge, due to the chemical and structural complexity of the cheese matrix, as well as the complexity of the biochemical reactions occurring during the fermentation and maturation processes. Microalgae are nutritious and sustainable food sources with important bioactive compounds such as proteins, polyunsaturated fatty acids, polysaccharides, chlorophylls, carotenoids, vitamins and minerals. Experimental approach This work aims to study the impact of the 2 and 4 % microalga Chlorella vulgaris addition on the nutritional composition, bioactivity, structure and sensory profile of quark and cream cheese, both probiotic fermented products. Texture profile analysis and fundamental rheology measurements (oscillatory and stationary) were performed to evaluate the impact of C. vulgaris on the mechanical properties of the fresh cheese. The nutritional composition was evaluated using standard methods and bioactivity through the determination of total phenolic compounds and antioxidant capacity.1 Results and conclusions C. vulgaris had an impact on the firmness of both cheeses. In general, the cheese with added C. vulgaris had a better nutritional profile, with an increase in protein content, content of Mg, P, S, Cu, Zn, Fe and Mn, and better bioactivity with an increase in the antioxidant activity. Sensory testing results were promising, especially for cream cheese. Novelty and scientific contribution The enrichment of traditional foods such as fresh cheese with microalgae represents an interesting strategy to develop hybrid products (with protein from animal and vegetable sources), obtain innovative and more sustainable products, and improve their nutritional profile in terms of protein and mineral content and bioactivity.

[1]  C. Prista,et al.  Innovative and Healthier Dairy Products through the Addition of Microalgae: A Review , 2022, Foods.

[2]  Issam Smaali,et al.  Effects of Growth Medium Variation on the Nutri-Functional Properties of Microalgae Used for the Enrichment of Ricotta , 2021, Food technology and biotechnology.

[3]  Inês Leitão,et al.  Response to stress induced by different potentially toxic elements (As, Cd, Cu and Na) in rapeseed leaves , 2021, Plant Physiology Reports.

[4]  A. Terpou,et al.  Influence of Incorporated Arthrospira (Spirulina) platensis on the Growth of Microflora and Physicochemical Properties of Feta-Type Cheese as Functional Food , 2020, Proceedings.

[5]  K. Elżbieta,et al.  Algae Chlorella vulgaris as a factor conditioning the survival of Lactobacillus spp. in adverse environmental conditions , 2020 .

[6]  M. C. Nunes,et al.  Tetraselmis chuii as a Sustainable and Healthy Ingredient to Produce Gluten-Free Bread: Impact on Structure, Colour and Bioactivity , 2020, Foods.

[7]  Y. Sung,et al.  Chlorella vulgaris: a perspective on its potential for combining high biomass with high value bioproducts , 2020 .

[8]  H. Pereira,et al.  Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae , 2019, Scientific Reports.

[9]  M. Hendrickx,et al.  The potential of microalgae and their biopolymers as structuring ingredients in food: A review. , 2019, Biotechnology advances.

[10]  M. Ghiaci,et al.  Phycocyanin-enriched yogurt and its antibacterial and physicochemical properties during 21 days of storage , 2019, LWT.

[11]  H. Shaaban,et al.  Production of functional spreadable processed cheese using Chlorella vulgaris. , 2018, Acta scientiarum polonorum. Technologia alimentaria.

[12]  A. Picon,et al.  Cheese supplementation with five species of edible seaweeds: Effect on microbiota, antioxidant activity, colour, texture and sensory characteristics , 2018, International Dairy Journal.

[13]  J. Costa,et al.  Spirulina for snack enrichment: Nutritional, physical and sensory evaluations , 2018 .

[14]  Antonio Miravete,et al.  Practical Analysis of COMPOSITE LAMINATES , 2018 .

[15]  D. Pleissner,et al.  Utilization of organic residues using heterotrophic microalgae and insects. , 2018, Waste management.

[16]  H. Attia,et al.  Effect of Spirulina platensis fortification on physicochemical, textural, antioxidant and sensory properties of yogurt during fermentation and storage , 2017 .

[17]  K. Kyriakopoulou,et al.  Effect of drying and extraction conditions on the recovery of bioactive compounds from Chlorella vulgaris , 2017, Journal of Applied Phycology.

[18]  K. Kyriakopoulou,et al.  Effect of drying and extraction conditions on the recovery of bioactive compounds from Chlorella vulgaris , 2017, Journal of Applied Phycology.

[19]  C. Chen,et al.  Phenolic Compounds: Functional Properties, Impact of Processing and Bioavailability , 2017 .

[20]  A. Picon,et al.  Seaweeds in yogurt and quark supplementation: influence of five dehydrated edible seaweeds on sensory characteristics , 2017 .

[21]  A. Porto,et al.  Effect of Aqueous Extract of the Seaweed Gracilaria domingensis on the Physicochemical, Microbiological, and Textural Features of Fermented Milks. , 2016, Journal of food science.

[22]  Xixi Cai,et al.  Purification and characterisation of a glutamic acid-containing peptide with calcium-binding capacity from whey protein hydrolysate , 2015, Journal of Dairy Research.

[23]  C. Santos-Buelga,et al.  Phenolic profiling of Veronica spp. grown in mountain, urban and sandy soil environments. , 2014, Food chemistry.

[24]  Kin Yan Lau,et al.  Recycling of food waste as nutrients in Chlorella vulgaris cultivation. , 2014, Bioresource technology.

[25]  Carlos Vaca-Garcia,et al.  Morphology, composition, production, processing and applications of Chlorella vulgaris: A review , 2014 .

[26]  J. Kerry,et al.  An examination of the potential of seaweed extracts as functional ingredients in milk , 2014 .

[27]  K. Khosravi‐Darani,et al.  Supplementation of Spirulina platensis and Chlorella vulgaris Algae into Probiotic Fermented Milks , 2013 .

[28]  K. K. Darani,et al.  Effects of Chlorella vulgaris and Arthrospira platensis addition on viability of probiotic bacteria in yogurt and its biochemical properties , 2012, European Food Research and Technology.

[29]  C. Pan,et al.  Effect of pressurized liquids on extraction of antioxidants from Chlorella vulgaris. , 2010, Journal of agricultural and food chemistry.

[30]  J. Fernández-López,et al.  The isolation and properties of a concentrated red-purple betacyanin food colourant from Opuntia stricta fruits , 2006 .

[31]  M. S. Miranda,et al.  Antioxidant activity of the microalga Chlorella vulgaris cultered on special conditions. , 2001, Bollettino chimico farmaceutico.

[32]  F. Gaucheron.  Iron fortification in dairy industry , 2000 .

[33]  H. Belshaw,et al.  The Food and Agriculture Organization of the United Nations , 1947, International Organization.

[34]  J. Pires,et al.  CO2 capture using microalgae , 2020 .

[35]  Felfoul,et al.  Investigation of physicochemical, nutritional, textural, and sensory properties of yoghurt fortified with fresh and dried Spirulina (Arthrospira platensis) , 2019 .

[36]  L. Uthira,et al.  Total phenolic content of organic and conventional green leafy vegetables , 2018 .

[37]  Kunal M. Gawai,et al.  Stabilizers, Colorants, and Exopolysaccharides in Yogurt , 2017 .

[38]  S. Shalaby.  Quality of Novel Healthy Processed Cheese Analogue Enhanced with Marine Microalgae Chlorella vulgaris Biomass , 2013 .

[39]  A. Meisterernst.  A Learning Process? – Three Years of Regulation (EC) No. 1924/2006 on Nutrition and Health Claims Made on Foods , 2010 .

[40]  Ana Paula Batista,et al.  Microalgae in novel food products , 2008 .

[41]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .

[42]  W. Sandritter,et al.  [Effect of hormones on experimental cervix carcinomas]. , 1967, Verhandlungen der Deutschen Gesellschaft fur Innere Medizin.