Overexpression of cyclin B1 antagonizes chemotherapeutic-induced apoptosis through PTEN/Akt pathway in human esophageal squamous cell carcinoma cells

The role of cyclin B1 in the clinical therapeutic sensitivity of human esophageal squamous cell carcinoma (ESCC) remains to be defined. In this study, we found that elevated cyclin B1 expression attenuated the apoptosis induced by cisplatin or paclitaxel, while knockdown of cyclin B1 enhanced cisplatin or paclitaxel sensitivity in ESCC cells. Cyclin B1-mediated apoptosis may rely on the Bcl-2-dependent mitochondria-regulated intrinsic death pathway, and the antagonizing effect of cyclin B1 on chemotherapeutic agent-induced apoptosis was through PTEN/Akt pathway. Therefore, cyclin B1 might be a therapeutic target for the development of specific and efficient approaches in the treatment of ESCC.

[1]  J. Yokota,et al.  Invasiveness and anchorage independent growth ability augmented by PTEN inactivation through the PI3K/AKT/NFkB pathway in lung cancer cells. , 2011, Lung cancer.

[2]  H. Broxmeyer,et al.  SIRT1 deficiency downregulates PTEN/JNK/FOXO1 pathway to block reactive oxygen species-induced apoptosis in mouse embryonic stem cells. , 2011, Stem cells and development.

[3]  H. Walczak,et al.  Caspase-8 and bid: caught in the act between death receptors and mitochondria. , 2011, Biochimica et biophysica acta.

[4]  G. Piazza,et al.  A High-Throughput Screen with Isogenic PTEN+/+ and PTEN−/− Cells Identifies CID1340132 as a Novel Compound That Induces Apoptosis in PTEN and PIK3CA Mutant Human Cancer Cells , 2011, Journal of biomolecular screening.

[5]  G. Pfeifer,et al.  Loss of Rassf1a enhances p53-mediated tumor predisposition and accelerates progression to aneuploidy , 2011, Oncogene.

[6]  R. Battafarano,et al.  Loss of p53, rather than beta-catenin overexpression, induces survivin-mediated resistance to apoptosis in an esophageal cancer cell line. , 2010, The Journal of thoracic and cardiovascular surgery.

[7]  N. Lin,et al.  Downregulation of N-cadherin Expression Inhibits Invasiveness, Arrests Cell Cycle and Induces Cell Apoptosis in Esophageal Squamous Cell Carcinoma , 2010, Cancer investigation.

[8]  H. Song,et al.  A multi-center phase II study of docetaxel plus cisplatin as first-line therapy in patients with metastatic squamous cell esophageal cancer , 2010, Cancer Chemotherapy and Pharmacology.

[9]  Stewart N Loh,et al.  Folding of tetrameric p53: oligomerization and tumorigenic mutations induce misfolding and loss of function. , 2010, Journal of molecular biology.

[10]  Xiongfei Zhang,et al.  Tissue inhibitor of metalloproteinase‐1 protects MCF‐7 breast cancer cells from paclitaxel‐induced apoptosis by decreasing the stability of cyclin B1 , 2010, International journal of cancer.

[11]  Q. Zhan,et al.  Stable knockdown of Aurora-A by vector-based RNA interference in human esophageal squamous cell carcinoma cell line inhibits tumor cell proliferation, invasion and enhances apoptosis , 2009, Cancer biology & therapy.

[12]  Q. Zhan,et al.  Overexpression of cyclin B1 in human esophageal squamous cell carcinoma cells induces tumor cell invasive growth and metastasis. , 2008, Carcinogenesis.

[13]  Y. Bae,et al.  Cytotoxicity of diacetoxyscirpenol is associated with apoptosis by activation of caspase-8 and interruption of cell cycle progression by down-regulation of cdk4 and cyclin B1 in human Jurkat T cells. , 2007, Toxicology and applied pharmacology.

[14]  C. Perez-stable,et al.  Increased expression of cyclin B1 sensitizes prostate cancer cells to apoptosis induced by chemotherapy , 2007, Molecular Cancer Therapeutics.

[15]  D. Schrump,et al.  Enhancement of Apo2L/TRAIL-mediated cytotoxicity in esophageal cancer cells by cisplatin , 2006, Molecular Cancer Therapeutics.

[16]  J. Hickman,et al.  Analysis of cyclin B1 and CDK activity during apoptosis induced by camptothecin treatment , 2006, Oncogene.

[17]  J. Yuan,et al.  Stable gene silencing of cyclin B1 in tumor cells increases susceptibility to taxol and leads to growth arrest in vivo , 2006, Oncogene.

[18]  Young Tae Kim,et al.  Loss of Cyclin B1 followed by downregulation of Cyclin A/Cdk2, apoptosis and antiproliferation in Hela cell line , 2005, International journal of cancer.

[19]  Andrew K Godwin,et al.  AKT and mTOR phosphorylation is frequently detected in ovarian cancer and can be targeted to disrupt ovarian tumor cell growth , 2004, Oncogene.

[20]  K. Strebhardt,et al.  Cyclin B1 depletion inhibits proliferation and induces apoptosis in human tumor cells , 2004, Oncogene.

[21]  L. Helman,et al.  Levels of PTEN protein modulate Akt phosphorylation on serine 473, but not on threonine 308, in IGF-II-overexpressing rhabdomyosarcomas cells , 2003, Oncogene.

[22]  M. V. Heiden,et al.  Caspase-9 is activated in a cytochrome c-independent manner early during TNFα-induced apoptosis in murine cells , 2003, Cell Death and Differentiation.

[23]  Ming-Rong Wang,et al.  [Establishment and comparative genomic hybridization analysis of human esophageal carcinomas cell line EC9706]. , 2002, Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics.

[24]  Diane D. Liu,et al.  Cyclin B1 overexpression and resistance to radiotherapy in head and neck squamous cell carcinoma. , 2002, Cancer research.

[25]  C. Sawyers,et al.  The phosphatidylinositol 3-Kinase–AKT pathway in human cancer , 2002, Nature Reviews Cancer.

[26]  S. Semba,et al.  Phosphorylation of Akt/PKB is required for suppression of cancer cell apoptosis and tumor progression in human colorectal carcinoma , 2002, Cancer.

[27]  N. Kunishima,et al.  Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[28]  J. Hayakawa,et al.  Inhibition of BAD phosphorylation either at serine 112 via extracellular signal-regulated protein kinase cascade or at serine 136 via Akt cascade sensitizes human ovarian cancer cells to cisplatin. , 2000, Cancer research.

[29]  W. Yung,et al.  Regulation of Akt/PKB activity, cellular growth, and apoptosis in prostate carcinoma cells by MMAC/PTEN. , 1999, Cancer research.

[30]  L. Cantley,et al.  New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[31]  Emad S. Alnemri,et al.  Ordering the Cytochrome c–initiated Caspase Cascade: Hierarchical Activation of Caspases-2, -3, -6, -7, -8, and -10 in a Caspase-9–dependent Manner , 1999, The Journal of cell biology.

[32]  A. Berchuck,et al.  PTEN mutation in endometrial cancers is associated with favorable clinical and pathologic characteristics. , 1998, Clinical cancer research : an official journal of the American Association for Cancer Research.

[33]  R. Eeles,et al.  Germline PTEN mutations in Cowden syndrome-like families. , 1998, Journal of medical genetics.

[34]  Junying Yuan,et al.  Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis , 1998, Cell.

[35]  D. Morgan,et al.  Nuclear Localization of Cyclin B1 Controls Mitotic Entry After DNA Damage , 1998, The Journal of cell biology.

[36]  John Calvin Reed.  Dysregulation of apoptosis in cancer. , 1998, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[37]  F. McCormick,et al.  Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B. , 1998, Science.

[38]  A. Berchuck,et al.  PTEN/MMAC1 mutations in endometrial cancers. , 1997, Cancer research.

[39]  M. Wigler,et al.  PTEN, a Putative Protein Tyrosine Phosphatase Gene Mutated in Human Brain, Breast, and Prostate Cancer , 1997, Science.

[40]  W. B. Derry,et al.  Mitotic block induced in HeLa cells by low concentrations of paclitaxel (Taxol) results in abnormal mitotic exit and apoptotic cell death. , 1996, Cancer research.

[41]  M. Imamura,et al.  Hyperthermic Enhancement of Cytotoxicity and Increased Uptake of cis‐Diamminedichloroplatinum(II) in Cultured Human Esophageal Cancer Cells , 1993, Japanese journal of cancer research : Gann.

[42]  S. Lippard,et al.  An intrastrand d(GpG) platinum crosslink in duplex M13 DNA is refractory to repair by human cell extracts. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[43]  J. Testa,et al.  A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. , 1991, Science.

[44]  N Kumar,et al.  Taxol-induced polymerization of purified tubulin. Mechanism of action. , 1981, The Journal of biological chemistry.

[45]  P. Schiff,et al.  Promotion of microtubule assembly in vitro by taxol , 1979, Nature.

[46]  Peter D Siersema,et al.  Esophageal cancer. , 2008, Gastroenterology clinics of North America.

[47]  A. Jemal,et al.  Cancer Statistics, 2005 , 2005, CA: a cancer journal for clinicians.

[48]  S. Horwitz,et al.  Mechanism of action of taxol. , 1992, Trends in pharmacological sciences.

[49]  N. Dubrawsky.  Cancer statistics , 1989, CA: a cancer journal for clinicians.

[50]  P. Leung,et al.  Reproductive Biology and Endocrinology Involvement of Cyclin B1 in Progesterone-mediated Cell Growth Inhibition, G2/m Cell Cycle Arrest, and Apoptosis in Human Endometrial Cell , 2022 .