The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants

The influence of light quality on growth and development of in vitro grown Doritaenopsis hort. (Orchidaceae) plants was investigated. Growth parameters like leaf and root fresh/dry mass and leaf area were highest with plants grown under red plus blue light emitting diodes (LEDs). Leaf length was greater with the plants grown under red LED. Carbohydrate (starch, sucrose, glucose and fructose) and leaf pigment (chlorophylls and carotenoids) biosynthesis of the plants was significantly increased in plants grown under red plus blue LEDs compared to red or blue LED and fluorescent light treatments. This study suggests that the production of quality Doritaenopsis plants is possible by culturing the plants in vitro under a mixture of blue plus red light sources.

[1]  J. Kennedy,et al.  Carbohydrate analysis: a practical approach , 1986 .

[2]  K. Paek,et al.  Sprouting rate, change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vitro , 2002 .

[3]  H. Watanabe,et al.  In vitro growth of Cymbidium plantlets cultured under superbright red and blue light-emitting diodes (LEDs) , 1998 .

[4]  K. Hughes.  In vitro ecology: Exogenous factors affecting growth and morphogenesis in plant culture systems , 1981 .

[5]  A. Schuerger,et al.  Anatomical features of pepper plants (Capsicum annuum L.) grown under red light-emitting diodes supplemented with blue or far-red light. , 1997, Annals of botany.

[6]  T. Kozai,et al.  Environmental control in plant tissue culture — general introduction and overview , 1995 .

[7]  C. Brown,et al.  Root-Shoot Interaction in the Greening of Wheat Seedlings Grown under Red Light , 1995, Plant physiology.

[8]  T. Sharkey,et al.  Light-emitting diodes as a light source for photosynthesis research , 2004, Photosynthesis Research.

[9]  So-Young Park,et al.  Rapid propagation of Phalaenopsis from floral stalk-derived leaves , 2002, In Vitro Cellular & Developmental Biology - Plant.

[10]  H. Lichtenthaler.  CHLOROPHYLL AND CAROTENOIDS: PIGMENTS OF PHOTOSYNTHETIC BIOMEMBRANES , 1987 .

[11]  J C Sager,et al.  Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. , 1995, Journal of the American Society for Horticultural Science. American Society for Horticultural Science.

[12]  T W Tibbitts,et al.  Growth of Lettuce, Spinach, Mustard, and Wheat Plants under Four Combinations of High-pressure Sodium, Metal Halide, and Tungsten Halogen Lamps at Equal PPFD , 1983, Journal of the American Society for Horticultural Science.

[13]  T. Kozai,et al.  Automation and environmental control in plant tissue culture , 1995, Springer Netherlands.