Unveiling of the epidemiological patterns for caprine/ovine enterovirus infection in China

Caprine/ovine enterovirus (CEV/OEV) infection is an emerging disease and remains largely unknown for its infection distribution, epidemic pattern, and the underlying contribution factors. Here, we report the investigation on CEV/OEV infection pattern and the underlying contribution factors by employing a sandwich ELISA kit for detection of CEV/OEV antigen. Epidemiological investigation revealed a wide range of infection rates of CEV/OEV from 19.80%−39.00% on goat/sheep farms in the major goat/sheep-raising provinces as such Henan, Shandong, Ningxia, Jilin, Inner Mongolia autonomous region, and Xinjiang autonomous region in China. Epidemic patterns and infection rates for CEV/OEV were affected by the breeds, raising mode, regions, and seasons. CEV/OEV infection rates were varied in different regions in China and significantly higher in the diarrheal herds (40.30%) than these in non-diarrheal herds (13.83%). Moreover, infection rate was higher in sheep (24.59%) than that in goats (9.84%), even dramatic difference among different breeds of goat or sheep. Out of different breeds of goat, Boer (20.13%) had the highest infection rate, followed by local breed (5.62%) and Saanen (2.61%). Among these breeds of sheep, higher infection rates were detected in local breed sheep (42.86%) and small-tailed Han sheep (35.91%) than these of Hu sheep (13.41%) and Dorper sheep (16.34%). Furthermore, raising modes were showed to contribute to the infection rate, where higher rates were detected among goats/sheep in captivity (27.10%) than these in free-range (12.27%) and semi-free range (19.24%). Additionally, CEV/OEV infection rate had obvious seasonality, while they increased from year 2015 to 2019. In summary, we investigated the CEV/OEV infection among the goat/sheep herds from different regions in China, revealed the epidemic pattern and the contribution factors to the infection, which provided the epidemiological data for future prevention and control of this emerging infection.

[1]  Xinping Wang,et al.  Molecular Analysis of Caprine Enterovirus Circulating in China during 2016–2021: Evolutionary Significance , 2022, Viruses.

[2]  E. Palmer,et al.  Picornaviridae , 2019, Electron Microscopy in Viral Diagnosis.

[3]  Xinping Wang,et al.  Discovery of a virus of the species Enterovirus F in goats , 2019, Archives of Virology.

[4]  I. Chavez,et al.  Molecular Identification of Enteroviruses from Cattle and Goat Feces and Environment in Thailand , 2018, Applied and Environmental Microbiology.

[5]  A. Mushegian,et al.  Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2018) , 2018, Archives of Virology.

[6]  R. L. Harrison,et al.  Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2018) , 2017, Archives of Virology.

[7]  R. Zell,et al.  High genetic diversity of porcine enterovirus G in Schleswig-Holstein, Germany , 2018, Archives of Virology.

[8]  P. Simmonds,et al.  ICTV Virus Taxonomy Profile: Picornaviridae , 2017, The Journal of general virology.

[9]  Changming Guo,et al.  A novel enterovirus species identified from severe diarrheal goats , 2017, PloS one.

[10]  S. Goyal,et al.  Isolation and molecular characterization of bovine enteroviruses in Egypt , 2015, The Veterinary Journal.

[11]  Xinping Wang,et al.  Identification of a Novel Enterovirus E Isolates HY12 from Cattle with Severe Respiratory and Enteric Diseases , 2014, PloS one.

[12]  B. Hause,et al.  First Identification and Characterization of Porcine Enterovirus G in the United States , 2014, PloS one.

[13]  T. Mizutani,et al.  Detection of enterovirus genome sequence from diarrheal feces of goat , 2014, Virus Genes.

[14]  A. Alfieri,et al.  First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil , 2014, Tropical Animal Health and Production.

[15]  G. Reuter,et al.  Natural interspecies recombinant bovine/porcine enterovirus in sheep. , 2012, The Journal of general virology.

[16]  Jitao Chang,et al.  Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes , 2012, Archives of Virology.

[17]  X. Hua,et al.  Prevalence of porcine enterovirus 9 in pigs in Middle and Eastern China , 2013, Virology Journal.

[18]  J. Saliki,et al.  Pathogenesis of a Bovine Enterovirus-1 Isolate in Experimentally Infected Calves , 2011, Veterinary pathology.

[19]  J. Saliki,et al.  Fatal Ulcerative and Hemorrhagic Typhlocolitis in a Pregnant Heifer Associated with Natural Bovine Enterovirus Type-1 Infection , 2007, Veterinary pathology.

[20]  Huang Cm,et al.  Bovine enteroviruses in the calf: an attempt at serologic, biologic, and pathologic classification. , 1974 .

[21]  H. W. Dunne,et al.  Bovine enteroviruses in the calf: an attempt at serologic, biologic, and pathologic classification. , 1974, Journal of the American Veterinary Medical Association.

[22]  O. Straub.  [Isolation of a bovine enterovirus from the genitalia]. , 1965, Deutsche tierarztliche Wochenschrift.

[23]  T. Moll,et al.  Biologic characteristics of certain bovine enteric viruses. , 1963, American journal of veterinary research.

[24]  T. Moll,et al.  Isolation and characterization of cytopathogenic enteroviruses from cattle with respiratory disease. , 1959, American journal of veterinary research.