Using Artificial Neural Networks for Optical Imaging of Fluorescent Biomarkers

The article presents the results of the application of artificial neural networks to separate the fluorescent contribution of nanodiamonds used as biomarkers, adsorbents and carriers of drugs in biomedicine, from a fluorescent background of own biological fluorophores. The principal possibility of solving this problem is shown. Use of neural network architecture let to detect fluorescence of nanodiamonds against the background autofluorescence of egg white with high accuracy better than 3 ug/ml. Keywords—Artificial neural networks, fluorescence, data aggregation.

[1]  M. Gross,et al.  Dark-field digital holographic microscopy for 3D-tracking of gold nanoparticles. , 2011, Optics express.

[2]  A. Wax,et al.  Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticles. , 2008, Journal of biomedical optics.

[3]  Miroslav D. Dramićanin,et al.  Artificial neural networks for processing fluorescence spectroscopy data in skin cancer diagnostics , 2013 .

[4]  Hua Jiang,et al.  Core-shell designs of photoluminescent nanodiamonds with porous silica coatings for bioimaging and drug delivery I: fabrication. , 2013, Journal of materials chemistry. B.

[5]  Miroslav D. Dramićanin,et al.  Optical Biopsy Method for Breast Cancer Diagnosis Based on Artificial Neural Network Classification οf Fluorescence Landscape Data , 2009 .

[6]  Nancy A. Monteiro-Riviere,et al.  Use of confocal microscopy for nanoparticle drug delivery through skin , 2012, Journal of biomedical optics.

[7]  Cecilia Sahlgren,et al.  Towards multifunctional, targeted drug delivery systems using mesoporous silica nanoparticles--opportunities & challenges. , 2010, Nanoscale.

[8]  Olga Shenderova,et al.  Photoluminescent Nanostructures from Graphite Oxidation , 2012 .

[9]  I. G. Persiantsev,et al.  New opportunity solutions to inverse problems in laser spectroscopy involving artificial neural networks , 2002 .

[10]  Hua Jiang,et al.  Core-shell designs of photoluminescent nanodiamonds with porous silica coatings for bioimaging and drug delivery II: application. , 2013, Nanoscale.

[11]  Sergey Burikov,et al.  Diamond−Water Coupling Effects in Raman and Photoluminescence Spectra of Nanodiamond Colloidal Suspensions , 2012 .

[12]  C Cremer,et al.  Considerations on a laser-scanning-microscope with high resolution and depth of field. , 1978, Microscopica acta.

[13]  A. Demchenko Introduction to Fluorescence Sensing , 2008, Springer International Publishing.

[14]  Matthieu Zellweger,et al.  Fluorescence spectroscopy of exogenous, exogenously-induced and endogenous fluorophores for the photodetection and photodynamic therapy of cancer , 2000 .

[15]  Tamitake Itoh,et al.  Semiconductor quantum dots and metal nanoparticles: syntheses, optical properties, and biological applications , 2008, Analytical and bioanalytical chemistry.

[16]  Saber M Hussain,et al.  Are diamond nanoparticles cytotoxic? , 2007, The journal of physical chemistry. B.

[17]  Haitian Yang,et al.  Solving inverse bimodular problems via artificial neural network , 2009 .

[18]  Huan-Cheng Chang,et al.  Nanodiamonds for optical bioimaging , 2010 .

[19]  Brijesh Verma,et al.  RBF neural networks for solving the inverse problem of backscattering spectra , 2008, Neural Computing and Applications.

[20]  Tatiana A. Dolenko,et al.  Carbon‐Dot‐Decorated Nanodiamonds , 2014 .

[21]  Amanda M. Schrand,et al.  Nanodiamond Particles: Properties and Perspectives for Bioapplications , 2009 .

[22]  I. G. Persiantsev,et al.  Comparison of Input Data Compression Methods in Neural Network Solution of Inverse Problem in Laser Raman Spectroscopy of Natural Waters , 2012, ICANN.

[23]  Luke D Lavis,et al.  Advances in the chemistry of small molecule fluorescent probes. , 2011, Current opinion in chemical biology.

[24]  Jessica M. Rosenholm,et al.  Study of adsorption properties of functionalized nanodiamonds in aqueous solutions of metal salts using optical spectroscopy , 2014 .

[25]  Dmitri O. Lapotko,et al.  Methods for monitoring and imaging nanoparticles in cells , 2007, SPIE BiOS.

[26]  Edward Keedwell,et al.  Intelligent Bioinformatics: The Application of Artificial Intelligence Techniques to Bioinformatics Problems , 2005 .

[27]  Ya‐Ping Sun,et al.  Carbon dots for multiphoton bioimaging. , 2007, Journal of the American Chemical Society.

[28]  Gereon Hüttmann,et al.  Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes. , 2008, Journal of biomedical optics.

[29]  Ya‐Ping Sun,et al.  Carbon "quantum" dots for optical bioimaging. , 2013, Journal of materials chemistry. B.

[30]  Daniel Evanko The new fluorescent probes on the block , 2008, Nature Methods.

[31]  Hsiao-Yun Wu,et al.  Characterization and application of single fluorescent nanodiamonds as cellular biomarkers , 2007, Proceedings of the National Academy of Sciences.