Antigens recognized by the human immune response to vaccination with a bivalent hardjo/pomona leptospiral vaccine.

Serum from volunteer subjects vaccinated with a bivalent whole cell vaccine of Leptospira interrogans serovar hardjo/serovar pomona grown in protein-free medium, was tested by the microscopic agglutination test (MAT), enzyme-immunoassay (EIA) and immunoblotting. Specific IgM antibodies to either serovars hardjo or pomona were detected in some subjects as early as 6 days after vaccination with peak antibody levels occurring 13-68 days after vaccination. Whereas all subjects produced specific IgM to both serovars, not all produced specific IgG to both serovars. Immunoblotting with hardjo sonicate revealed that all subjects produced IgM antibodies reacting with the 15, 23 and 28 kDa components of hardjo lipopolysaccharide (LPS), and most produced IgM antibodies that reacted with the 34.5 kDa flagellar doublet. In contrast, not all sera immunoblotted against pomona sonicate reacted with the 29 and 35 kDa components of pomona LPS. However all subjects produced antibodies reacting with a diffuse 14.4-27 kDa band. These antibodies appeared early in the immune response. Serum from the one vaccinated subject tested protected hamsters from acute lethal infection with serovar pomona.

[1]  B. Adler,et al.  Monoclonal antibodies reacting with serogroup and serovar specific epitopes on different lipopolysaccharide subunits of Leptospira interrogans serovar pomona. , 1989, FEMS microbiology immunology.

[2]  J. Pagés,et al.  Immunological evidence for differences in the exposed regions of OmpF porins from Escherichia coli B and K-12. , 1988, Molecular immunology.

[3]  B. Adler,et al.  Antigens recognised by the human immune response to infection with Leptospira interrogans serovar hardjo. , 1988, Journal of medical microbiology.

[4]  S Faine,et al.  Opsonic monoclonal antibodies against lipopolysaccharide antigens of Leptospira interrogans serovar hardjo. , 1987, Journal of medical microbiology.

[5]  S Faine,et al.  A monoclonal antibody reacting with a determinant on leptospiral lipopolysaccharide protects guinea pigs against leptospirosis. , 1986, Journal of medical microbiology.

[6]  I. Smart,et al.  Enzyme-linked immunosorbent assay for determining specific immunoglobulin M in infections caused by Leptospira interrogans serovar hardjo , 1985, Journal of clinical microbiology.

[7]  B. Adler,et al.  Fatal congenital human leptospirosis. , 1984, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology.

[8]  B. Adler,et al.  PHAGOCYTOSIS OF VIRULENT AND AVIRULENT LEPTOSPIRES BY GUINEA‐PIG AND HUMAN POLYMORPHONUCLEAR LEUKOCYTES IN VITRO , 1984, Pathology.

[9]  C. Wilks,et al.  Human leptospirosis in Victoria , 1983, The Medical journal of Australia.

[10]  B. Adler,et al.  Immunogenicity of leptospiral vaccines grown in protein-free medium. , 1982, Journal of medical microbiology.

[11]  Blackmore Dk,et al.  Risks of contracting leptospirosis on the dairy farm. , 1982 .

[12]  D. Blackmore,et al.  Risks of contracting leptospirosis on the dairy farm. , 1982, The New Zealand medical journal.

[13]  S. Faine.  Guidelines for the control of leptospirosis. , 1982, WHO offset publication.

[14]  N. Cook,et al.  Epidemiology of leptospirosis in dairy farm workers in the Manawatu. Part I: A cross-sectional serological survey and associated occupational factors. , 1980, New Zealand veterinary journal.

[15]  G. Schoone,et al.  Serodiagnosis of human leptospirosis by enzyme-linked-immunosorrbent-assay (ELISA). , 1980, Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie.

[16]  S. Locarnini,et al.  Detection of specific anti-leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay , 1980, Journal of clinical microbiology.

[17]  H. Muller,et al.  MATURATION OF HUMORAL IMMUNE RESPONSE DETERMINES THE SUSCEPTIBILITY OF GUINEA‐PIGS TO LEPTOSPIROSIS , 1980, Pathology.

[18]  B. Adler,et al.  The antibodies involved in the human immune response to leptospiral infection. , 1978, Journal of medical microbiology.

[19]  B. Adler,et al.  Host immunological mechanisms in the resistance of mice to leptospiral infections , 1977, Infection and immunity.

[20]  B. Adler,et al.  Susceptibility of mice treated with cyclophosphamide to lethal infection with Leptospira interrogans Serovar pomona , 1976, Infection and immunity.

[21]  A. Kaufmann.  EPIDEMIOLOGIC TRENDS OF LEPTOSPIROSIS IN THE UNITED STATES, 1965–1974 , 1976 .

[22]  Christmas Bw,et al.  Dairy farm fever in New Zealand: isolation of L pomona and L hardjo from a local outbreak. , 1974 .

[23]  J. Bragger,et al.  Dairy farm fever in New Zealand: isolation of L pomona and L hardjo from a local outbreak. , 1974, The New Zealand medical journal.

[24]  M. Torten,et al.  A new leptospiral vaccine for use in man. I. Development of a vaccine from Leptospira grown on a chemically defined medium. , 1973, The Journal of infectious diseases.

[25]  A. Palit,et al.  Genus-specific leptospiral antigen and its possible use in laboratory diagnosis , 1973, Journal of clinical pathology.

[26]  C. D. Cox,et al.  Evaluation of the hemolytic test in the serodiagnosis of human leptospirosis. , 1957, The Journal of infectious diseases.

[27]  Doherty Rl.  A second infection with leptospirosis. , 1956 .