Use of negative dielectrophoresis for selective elution of protein-bound particles.

In this paper with the aid of negative dielectrophoresis force in conjunction with shear force and at an optimal sodium hydroxide concentration we demonstrated a switchlike functionality to elute specifically bound beads from the surface. At an optimal flow rate and sodium hydroxide concentration, negative dielectrophoresis turned on results in bead detachment, whereas when negative dielectrophoresis is off, the beads remain attached. This platform offers the potential for performing a bead-based multiplexed assay where in a single channel various regions are immobilized with a different antibody, each targeting a different antigen. To develop the proof of concept and to demonstrate the switchlike functionality in eluting specifically bound beads from the surface we looked at two different protein interactions. We chose interactions that were in the same order of magnitude in strength as typical antibody-antigen interactions. The first was protein G-IgG interaction, and the second was the interaction between anti-IgG and IgG.

[1]  P. Hesketh,et al.  Rapid, low cost microfabrication technologies toward realization of devices for dielectrophoretic manipulation of particles and nanowires , 2006 .

[2]  J. Voldman,et al.  Holding forces of single-particle dielectrophoretic traps. , 2001, Biophysical journal.

[3]  W. N. Burnette,et al.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. , 1981, Analytical biochemistry.

[4]  Hsueh-Chia Chang,et al.  An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting. , 2007, Biomicrofluidics.

[5]  S. Quake,et al.  Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.

[6]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[7]  Gavin MacBeath,et al.  State-based discovery: a multidimensional screen for small-molecule modulators of EGF signaling , 2006, Nature Methods.

[8]  Tomoyuki Yasukawa,et al.  Negative dielectrophoretic patterning with different cell types. , 2008, Biosensors & bioelectronics.

[9]  H. Shiku,et al.  Rapid and separation-free sandwich immunosensing based on accumulation of microbeads by negative-dielectrophoresis. , 2008, Biosensors & bioelectronics.

[10]  M Javanmard,et al.  Microfluidic force spectroscopy for characterization of biomolecular interactions with piconewton resolution. , 2010, Applied physics letters.

[11]  Simon Goldbard,et al.  High-throughput cell analysis using multiplexed array technologies. , 2002, Drug discovery today.

[12]  G. Whitesides,et al.  Fabrication of microfluidic systems in poly(dimethylsiloxane) , 2000, Electrophoresis.

[13]  Sang Woo Lee,et al.  Dielectrophoretic technique for measurement of chemical and biological interactions. , 2009, Analytical chemistry.

[14]  R. Howe,et al.  Magnetic nanoparticle-driven pumping in microchannels , 2009, TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference.

[15]  H. Morgan,et al.  Dielectrophoretic Characterization and Separation of Antibody-Coated Submicrometer Latex Spheres , 1999 .

[16]  Philip A. Voglewede,et al.  An analysis of interdigitated electrode geometry for dielectrophoretic particle transport in micro-fluidics , 2007 .

[17]  M. Snyder,et al.  Protein arrays and microarrays. , 2001, Current opinion in chemical biology.

[18]  David F. Smith,et al.  Shotgun Glycomics: A Microarray Strategy for Functional Glycomics , 2010, Nature Methods.

[19]  H. Kitano.  Systems Biology: A Brief Overview , 2002, Science.

[20]  Ronald W. Davis,et al.  Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray , 1995, Science.

[21]  Juan G. Santiago,et al.  A planar electroosmotic micropump , 2002 .

[22]  D. Akin,et al.  Characterization and modeling of a microfluidic dielectrophoresis filter for biological species , 2005, Journal of Microelectromechanical Systems.

[23]  J. Voldman,et al.  High-throughput positive-dielectrophoretic bioparticle microconcentrator. , 2006, Analytical chemistry.

[24]  Stuart L. Schreiber,et al.  Printing Small Molecules as Microarrays and Detecting Protein−Ligand Interactions en Masse , 1999 .

[25]  Steven A Carr,et al.  Protein biomarker discovery and validation: the long and uncertain path to clinical utility , 2006, Nature Biotechnology.