EXPRESIÓN DE MOLÉCULAS B7 Y TLR:9 EN CÉLULAS EPITELIALES CORNEALES INFECTADAS CON ADENOVIRUS: IMPLICACIONES CLÍNICO-PATOLÓGICAS EN LA QUERATOCONJUNTIVITIS VIRAL EXPRESSION OF B7 MOLECULES AND TLR-9 ON CORNEAL EPITHELIAL CELLS INFECTED WITH ADENOVIRUS: CLINICO- PATHOLOGICAL IMPLICATIONS IN VIRAL KERA

Purpose: B7 molecules are a family of proteins that co-stimulate T cells during immune activation. Normally the corneal epithelial cells (CEC) do not express these molecules on their cell surface. Tolllike receptors play an important role in the innate immune response to invading pathogens and recently have been demonstrated to be expressed on mice cornea. The objective of this study was to determine whether adenoviral infection induces B7 molecules and TLR9 on human CEC. Methods: CEC were isolated from human corneas treated with dispase-II, and grown in the presence of supplemented hormonal epithelial medium until confluence. Then CEC were then infected with adenovirus 5 (Ad5) and cultured for different times.

[1]  Lieping Chen,et al.  Constitutive and inducible expression of b7 family of ligands by human airway epithelial cells. , 2005, American journal of respiratory cell and molecular biology.

[2]  T. Ganz,et al.  TGF-α Regulates TLR Expression and Function on Epidermal Keratinocytes1 , 2005, The Journal of Immunology.

[3]  C. Haidaris,et al.  Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens. , 2005, The Journal of allergy and clinical immunology.

[4]  D. Golenbock,et al.  Activation of toll-like receptor (TLR)2, TLR4, and TLR9 in the mammalian cornea induces MyD88-dependent corneal inflammation. , 2005, Investigative ophthalmology & visual science.

[5]  Shizuo Akira,et al.  Toll-like receptor signalling , 2004, Nature Reviews Immunology.

[6]  Lisa A. Beck,et al.  Keratinocytes in Atopic dermatitis: Inflammatory signals , 2004, Current allergy and asthma reports.

[7]  D. Curiel,et al.  Adenovirus serotype 3 utilizes CD80 (B7.1) and CD86 (B7.2) as cellular attachment receptors. , 2004, Virology.

[8]  J. Streilein.  New thoughts on the immunology of corneal transplantation , 2003, Eye.

[9]  J. Jester,et al.  Modulation of cultured corneal keratocyte phenotype by growth factors/cytokines control in vitro contractility and extracellular matrix contraction. , 2003, Experimental eye research.

[10]  J. Streilein.  Ocular immune privilege: therapeutic opportunities from an experiment of nature , 2003, Nature Reviews Immunology.

[11]  Zhenghong Yuan,et al.  Toll‐like receptor‐9 induced by physical trauma mediates release of cytokines following exposure to CpG motif in mouse skin , 2003, Immunology.

[12]  T. Bieber,et al.  The good, the bad and the ugly--APCs of the eye. , 2003, Trends in immunology.

[13]  S. Janssens,et al.  Role of Toll-Like Receptors in Pathogen Recognition , 2003, Clinical Microbiology Reviews.

[14]  M. Dana,et al.  Corneal immunity is mediated by heterogeneous population of antigen‐presenting cells , 2003, Journal of leukocyte biology.

[15]  N. Koizumi,et al.  Comparison of ultrastructure, tight junction-related protein expression and barrier function of human corneal epithelial cells cultivated on amniotic membrane with and without air-lifting. , 2003, Experimental eye research.

[16]  Claus Cursiefen,et al.  Corneal Lymphangiogenesis: Evidence, Mechanisms, and Implications for Corneal Transplant Immunology , 2003, Cornea.

[17]  J. Bremner,et al.  Epidemic kerato-conjunctivitis—do outbreaks have to be epidemic? , 2003, Eye.

[18]  M. Dana,et al.  The corneal stroma is endowed with a significant number of resident dendritic cells. , 2003, Investigative ophthalmology & visual science.

[19]  J. Allison,et al.  Cutting Edge: A Crucial Role for B7-CD28 in Transmitting T Help from APC to CTL1 , 2002, The Journal of Immunology.

[20]  J. Streilein,et al.  ANTERIOR CHAMBER ASSOCIATED IMMUNE DEVIATION (ACAID): REGULATION, BIOLOGICAL RELEVANCE, AND IMPLICATIONS FOR THERAPY , 2002, International reviews of immunology.

[21]  B. Rouse,et al.  Bystander activation of CD4+ T cells accounts for herpetic ocular lesions. , 2000, Investigative ophthalmology & visual science.

[22]  C. Foster,et al.  Cytokine mRNA in BALB/c mouse corneas infected with herpes simplex virus , 1999, Eye.

[23]  D. Nokes,et al.  A simplified and standardized neutralization enzyme immunoassay for the quantification of measles neutralizing antibody. , 1999, Journal of virological methods.

[24]  J. Streilein,et al.  Alterations in cytokine production following intraocular injection of soluble protein antigen: impairment in IFN-gamma and induction of TGF-beta and IL-4 production. , 1998, Journal of immunology.

[25]  T. Kivelä,et al.  Structure, development and function of cytoskeletal elements in non-neuronal cells of the Human Eye , 1998, Progress in Retinal and Eye Research.

[26]  J. Pepose,et al.  CD95 ligand (FasL)-induced apoptosis is necessary for corneal allograft survival. , 1997, The Journal of clinical investigation.

[27]  S. Ward.  CD28: a signalling perspective. , 1996, The Biochemical journal.

[28]  P. McDonnell,et al.  Studies of adenovirus-induced eye disease in the rabbit model. , 1995, Investigative ophthalmology & visual science.

[29]  P. McDonnell,et al.  Role of Adenovirus Type 5 Early Region 3 in the Pathogenesis of Ocular Disease and Cell Culture Infection , 1995, Cornea.

[30]  E. Romanowski,et al.  An ocular model of adenovirus type 5 infection in the NZ rabbit. , 1992, Investigative Ophthalmology and Visual Science.

[31]  J. Streilein,et al.  Characterization of suppressor cells in anterior chamber-associated immune deviation (ACAID) induced by soluble antigen. Evidence of two functionally and phenotypically distinct T-suppressor cell populations. , 1990, Immunology.

[32]  T. Ganz,et al.  TGF-alpha regulates TLR expression and function on epidermal keratinocytes. , 2005, Journal of immunology.

[33]  M. Johansson,et al.  Adenoviruses isolated in the Stockholm area during 1987–1992: restriction endonuclease analysis and molecular epidemiology , 2005, Archives of Virology.

[34]  W. Elliott,et al.  Latent adenoviral infection induces production of growth factors relevant to airway remodeling in COPD. , 2004, American journal of physiology. Lung cellular and molecular physiology.

[35]  Y. Huang,et al.  Interleukin-1alpha released from epithelial cells after adenovirus type 37 infection activates intercellular adhesion molecule 1 expression on human vascular endothelial cells. , 2002, Journal of virology.

[36]  Y. Huang,et al.  Interleukin-1 (cid:2) Released from Epithelial Cells after Adenovirus Type 37 Infection Activates Intercellular Adhesion Molecule 1 Expression on Human Vascular Endothelial Cells , 2001 .

[37]  G. Seong,et al.  TGF-beta s stimulate cell proliferation via an autocrine production of FGF-2 in corneal stromal fibroblasts. , 1998, Current eye research.

[38]  M. Trousdale,et al.  Adenovirus infection of the cornea causes histopathologic changes in the lacrimal gland. , 1997, Current eye research.

[39]  Nobuo Yamada,et al.  Generation of mature dendritic cells from a CD14+ cell line (XS52) by IL-4, TNF-alpha, IL-1 beta, and agonistic anti-CD40 monoclonal antibody. , 1999, Journal of immunology.