Clinical multiphoton tomography

Clinical multiphoton tomography and two-photon microendoscopy provide clinicians and researchers with high-resolution in vivo optical biopsies based on two-photon autofluorescence, second harmonic generation, and fluorescence lifetime imaging. This review reflects state of the art technology and reports on applications in the fields of early stage melanoma detection, skin aging, nanoparticle imaging, tissue engineering, and in situ screening of pharmaceutical and cosmetical products. So far, more than 500 patients and volunteers in Europe, Asia, and Australia have been investigated with these novel molecular imaging tools.

[1]  B R Masters,et al.  Multiphoton Excitation Microscopy of In Vivo Human Skin: Functional and Morphological Optical Biopsy Based on Three‐Dimensional Imaging, Lifetime Measurements and Fluorescence Spectroscopy a , 1998, Annals of the New York Academy of Sciences.

[2]  Karsten König,et al.  Multiphoton microscopy for the investigation of dermal penetration of nanoparticle-borne drugs. , 2006, The Journal of investigative dermatology.

[3]  Chen-Yuan Dong,et al.  Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium. , 2003, Journal of biomedical optics.

[4]  B R Masters,et al.  Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin. , 1997, Biophysical journal.

[5]  R. Wepf,et al.  Dead but Highly Dynamic – The Stratum corneum Is Divided into Three Hydration Zones , 2004, Skin Pharmacology and Physiology.

[6]  I. Riemann,et al.  In vivo Drug Screening in Human Skin Using Femtosecond Laser Multiphoton Tomography , 2006, Skin Pharmacology and Physiology.

[7]  Karsten König,et al.  In vivo assessment of human skin aging by multiphoton laser scanning tomography. , 2006, Optics letters.

[8]  P. So,et al.  Two-Photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures. , 1998, Optics express.

[9]  K König,et al.  Clinical two‐photon microendoscopy , 2007, Microscopy research and technique.

[10]  W. Denk,et al.  Two-photon laser scanning fluorescence microscopy. , 1990, Science.

[11]  Alf Giese,et al.  Multiphoton Excitation of Autofluorescence for Microscopy of Glioma Tissue , 2006, Neurosurgery.

[12]  K. König,et al.  Multiphoton autofluorescence imaging of intratissue elastic fibers. , 2005, Biomaterials.

[13]  K. König,et al.  Multiphoton microscopy in life sciences , 2000, Journal of microscopy.

[14]  R. Webb,et al.  Video-rate confocal scanning laser microscope for imaging human tissues in vivo. , 1999, Applied optics.

[15]  Karsten König,et al.  Multiphoton fluorescence lifetime imaging of human hair , 2007, Microscopy research and technique.