Recent engineering advances of EVs for compounds, nucleic acids, and TCM delivery.

[1]  A. Tutt,et al.  Serum-derived extracellular vesicles from breast cancer patients contribute to differential regulation of T-cell-mediated immune-escape mechanisms in breast cancer subtypes , 2023, Frontiers in Immunology.

[2]  Jing Wang,et al.  Latest advances and perspectives of liquid biopsy for cancer diagnostics driven by microfluidic on-chip assays. , 2023, Lab on a chip.

[3]  M. Pfaffl,et al.  The potential role of serum extracellular vesicle derived small RNAs in AML research as non-invasive biomarker , 2023, Nanoscale advances.

[4]  P. Brun,et al.  Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications , 2022, Biosensors.

[5]  P. Arosio,et al.  High-Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates. , 2022, Small.

[6]  Mingxiao Cui,et al.  Advanced research on extracellular vesicles based oral drug delivery systems. , 2022, Journal of controlled release : official journal of the Controlled Release Society.

[7]  M. M. Mc Gee,et al.  Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas , 2022, PloS one.

[8]  M. Hackenberg,et al.  Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies , 2022, Biomarker Research.

[9]  Huidongzi Xiao,et al.  Astragaloside IV drug-loaded exosomes (AS-IV EXOs) derived from endothelial progenitor cells improve the viability and tube formation in high-glucose impaired human endothelial cells by promoting miR-214 expression. , 2022, Endokrynologia Polska.

[10]  Lesley Cheng,et al.  Therapeutically harnessing extracellular vesicles , 2022, Nature Reviews Drug Discovery.

[11]  Ruoning Wang,et al.  Tumor-derived extracellular vesicles as messengers of natural products in cancer treatment , 2022, Theranostics.

[12]  Yan Wang,et al.  Protective effect of Yangxue Jiedu Soup against psoriasis-like lesions by regulating TLR4/NF-κB signaling pathway mediated by secretion of exosome HSP70. , 2022, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[13]  L. Du,et al.  Review on Strategies and Technologies for Exosome Isolation and Purification , 2022, Frontiers in Bioengineering and Biotechnology.

[14]  V. Wong,et al.  Extracellular Vesicle Delivery of Neferine for the Attenuation of Neurodegenerative Disease Proteins and Motor Deficit in an Alzheimer’s Disease Mouse Model , 2022, Pharmaceuticals.

[15]  Xiaomei Yan,et al.  Active cargo loading into extracellular vesicles: Highlights the heterogeneous encapsulation behaviour , 2021, Journal of extracellular vesicles.

[16]  R. Ouellette,et al.  The polysaccharide chitosan facilitates the isolation of small extracellular vesicles from multiple biofluids , 2021, Journal of extracellular vesicles.

[17]  J. Wolfram,et al.  Extracellular vesicle therapeutics from plasma and adipose tissue. , 2021, Nano today.

[18]  M. J. Wood,et al.  Extracellular vesicles as a next-generation drug delivery platform , 2021, Nature Nanotechnology.

[19]  J. Lötvall,et al.  Isolation and characterization of extracellular vesicle subpopulations from tissues , 2021, Nature Protocols.

[20]  Li Duan,et al.  Engineering exosomes for targeted drug delivery , 2021, Theranostics.

[21]  Nicholas A. Peppas,et al.  Engineering precision nanoparticles for drug delivery , 2020, Nature reviews. Drug discovery.

[22]  M. Riekkola,et al.  Modern isolation and separation techniques for extracellular vesicles. , 2020, Journal of chromatography. A.

[23]  J. Sluijter,et al.  Extracellular Vesicle-Associated Proteins in Tissue Repair. , 2020, Trends in cell biology.

[24]  M. Ratajczak,et al.  Extracellular microvesicles/exosomes: discovery, disbelief, acceptance, and the future? , 2020, Leukemia.

[25]  S. Du,et al.  Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications , 2020, International journal of nanomedicine.

[26]  Jing Ji,et al.  Bu-Shen-Yi-Sui Capsule, an Herbal Medicine Formula, Promotes Remyelination by Modulating the Molecular Signals via Exosomes in Mice with Experimental Autoimmune Encephalomyelitis , 2020, Oxidative medicine and cellular longevity.

[27]  Yuan Zhou,et al.  Zhen-Wu-Tang Protects IgA Nephropathy in Rats by Regulating Exosomes to Inhibit NF-κB/NLRP3 Pathway , 2020, Frontiers in Pharmacology.

[28]  S. Ramalingam,et al.  Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics , 2020, bioRxiv.

[29]  L. Ferreira,et al.  Native and bioengineered extracellular vesicles for cardiovascular therapeutics , 2020, Nature Reviews Cardiology.

[30]  S. Aryal,et al.  Surface functionalization strategies of extracellular vesicles. , 2020, Journal of materials chemistry. B.

[31]  Rui Wei,et al.  Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles , 2020, Biological Procedures Online.

[32]  M. Fussenegger,et al.  Shedding Light on Extracellular Vesicle Biogenesis and Bioengineering , 2020, Advanced science.

[33]  W. Rhee,et al.  Development and comparative analysis of human urine exosome isolation strategies , 2020, Process Biochemistry.

[34]  H. Lenz,et al.  Genetically Engineered Cell-Derived Nanoparticles for Targeted Breast Cancer Immunotherapy. , 2019, Molecular therapy : the journal of the American Society of Gene Therapy.

[35]  Betty Y. S. Kim,et al.  Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation , 2019, Nature Biomedical Engineering.

[36]  A. Gaharwar,et al.  Engineered Extracellular Vesicles with Synthetic Lipids via Membrane Fusion to Establish Efficient Gene Delivery. , 2019, International journal of pharmaceutics.

[37]  Heedoo Lee,et al.  Isolation and characterization of extracellular vesicles from Broncho-alveolar lavage fluid: a review and comparison of different methods , 2019, Respiratory research.

[38]  Y. Duan,et al.  The Effect of Triptolide-Loaded Exosomes on the Proliferation and Apoptosis of Human Ovarian Cancer SKOV3 Cells , 2019, BioMed research international.

[39]  M. Haris,et al.  Extracellular vesicles-mediated intercellular communication: roles in the tumor microenvironment and anti-cancer drug resistance , 2019, Molecular Cancer.

[40]  M. Brizzi,et al.  Improved Loading of Plasma-Derived Extracellular Vesicles to Encapsulate Antitumor miRNAs , 2019, Molecular therapy. Methods & clinical development.

[41]  I. Nazarenko,et al.  3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles , 2018, Advanced science.

[42]  A. Pitkänen,et al.  Precipitation-based extracellular vesicle isolation from rat plasma co-precipitate vesicle-free microRNAs , 2018, Journal of extracellular vesicles.

[43]  D. Dickson,et al.  Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid , 2018, Cells.

[44]  H. Lenz,et al.  Reprogramming Exosomes as Nanoscale Controllers of Cellular Immunity. , 2018, Journal of the American Chemical Society.

[45]  C. Qin,et al.  Exosome–transmitted long non-coding RNA PTENP1 suppresses bladder cancer progression , 2018, Molecular Cancer.

[46]  Q. Tang,et al.  NRP-1 targeted and cargo-loaded exosomes facilitate simultaneous imaging and therapy of glioma in vitro and in vivo. , 2018, Biomaterials.

[47]  H. Moulton,et al.  Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy , 2018, Science Translational Medicine.

[48]  Honglin Jin,et al.  Methotrexate-Loaded Extracellular Vesicles Functionalized with Therapeutic and Targeted Peptides for the Treatment of Glioblastoma Multiforme. , 2018, ACS applied materials & interfaces.

[49]  Hong-xia Yu,et al.  Suxiao Jiuxin pill promotes exosome secretion from mouse cardiac mesenchymal stem cells in vitro , 2018, Acta Pharmacologica Sinica.

[50]  Hong-xia Yu,et al.  Exosomes from Suxiao Jiuxin pill-treated cardiac mesenchymal stem cells decrease H3K27 demethylase UTX expression in mouse cardiomyocytes in vitro , 2018, Acta Pharmacologica Sinica.

[51]  E. Maffini,et al.  Extracellular vesicles as potential biomarkers of acute graft-vs-host disease , 2018, Leukemia.

[52]  Yanliang Zhu,et al.  Engineered exosome-mediated delivery of functionally active miR-26a and its enhanced suppression effect in HepG2 cells , 2018, International journal of nanomedicine.

[53]  Lijia Huang,et al.  Exosome–Liposome Hybrid Nanoparticles Deliver CRISPR/Cas9 System in MSCs , 2018, Advanced science.

[54]  Graça Raposo,et al.  Shedding light on the cell biology of extracellular vesicles , 2018, Nature Reviews Molecular Cell Biology.

[55]  J. Klein,et al.  Isolation and characterization of urinary extracellular vesicles: implications for biomarker discovery , 2017, Nature Reviews Nephrology.

[56]  Peixuan Guo,et al.  Nanoparticle Orientation to Control RNA Loading and Ligand Display on Extracellular Vesicles for Cancer Regression , 2017, Nature Nanotechnology.

[57]  M. Goumans,et al.  Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation. , 2017, Nanomedicine : nanotechnology, biology, and medicine.

[58]  Liping Xu,et al.  Tongxinluo Inhibits Renal Fibrosis in Diabetic Nephropathy: Involvement of the Suppression of Intercellular Transfer of TGF-[Formula: see text]1-Containing Exosomes from GECs to GMCs. , 2017, The American journal of Chinese medicine.

[59]  Subbarao Bondada,et al.  Milk-derived exosomes for oral delivery of paclitaxel. , 2017, Nanomedicine : nanotechnology, biology, and medicine.

[60]  K. Ishii,et al.  CD63-Mediated Antigen Delivery into Extracellular Vesicles via DNA Vaccination Results in Robust CD8+ T Cell Responses , 2017, The Journal of Immunology.

[61]  F. Dignat-George,et al.  Extracellular Vesicles in Angiogenesis , 2017, Circulation research.

[62]  Raghu Kalluri,et al.  Exosomes Facilitate Therapeutic Targeting of Oncogenic Kras in Pancreatic Cancer , 2017, Nature.

[63]  Xin Luan,et al.  Engineering exosomes as refined biological nanoplatforms for drug delivery , 2017, Acta Pharmacologica Sinica.

[64]  Diego Diez,et al.  A novel affinity-based method for the isolation of highly purified extracellular vesicles , 2016, Scientific Reports.

[65]  E. Bieberich,et al.  Neutral Sphingomyelinase-2 Deficiency Ameliorates Alzheimer's Disease Pathology and Improves Cognition in the 5XFAD Mouse , 2016, The Journal of Neuroscience.

[66]  X. Gallart‐Palau,et al.  Enrichment of extracellular vesicles from tissues of the central nervous system by PROSPR , 2016, Molecular Neurodegeneration.

[67]  Yoshihiro Sasaki,et al.  Engineering hybrid exosomes by membrane fusion with liposomes , 2016, Scientific Reports.

[68]  Myung Soo Kim,et al.  Using exosomes, naturally-equipped nanocarriers, for drug delivery. , 2015, Journal of controlled release : official journal of the Controlled Release Society.

[69]  Qiaobing Xu,et al.  Integrating Protein Engineering and Bioorthogonal Click Conjugation for Extracellular Vesicle Modulation and Intracellular Delivery , 2015, PloS one.

[70]  Richa Gupta,et al.  Exosomes as drug delivery vehicles for Parkinson's disease therapy. , 2015, Journal of controlled release : official journal of the Controlled Release Society.

[71]  L. Schaeffer,et al.  Efficient inhibition of infectious prions multiplication and release by targeting the exosomal pathway , 2015, Cellular and Molecular Life Sciences.

[72]  Henrik J Johansson,et al.  Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. , 2015, Nanomedicine : nanotechnology, biology, and medicine.

[73]  A. Möller,et al.  Optimized exosome isolation protocol for cell culture supernatant and human plasma , 2015, Journal of extracellular vesicles.

[74]  Silvia Maria Doglia,et al.  Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: a new approach for drug delivery. , 2014, Journal of controlled release : official journal of the Controlled Release Society.

[75]  Richa Gupta,et al.  GDNF-Transfected Macrophages Produce Potent Neuroprotective Effects in Parkinson's Disease Mouse Model , 2014, PloS one.

[76]  Thomas J. Anchordoquy,et al.  Surface Functionalization of Exosomes Using Click Chemistry , 2014, Bioconjugate chemistry.

[77]  Meg Duroux,et al.  A comprehensive overview of exosomes as drug delivery vehicles - endogenous nanocarriers for targeted cancer therapy. , 2014, Biochimica et biophysica acta.

[78]  P. Robbins,et al.  Regulation of immune responses by extracellular vesicles , 2014, Nature Reviews Immunology.

[79]  J. Vandesompele,et al.  The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling , 2014, Journal of extracellular vesicles.

[80]  A. Hellvard,et al.  The 2013 Nobel Prize in Physiology or Medicine , 2013, Scandinavian journal of immunology.

[81]  M. V. Filatov,et al.  Exosomes are natural carriers of exogenous siRNA to human cells in vitro , 2013, Cell Communication and Signaling.

[82]  Jaesung Park,et al.  Bioinspired exosome-mimetic nanovesicles for targeted delivery of chemotherapeutics to malignant tumors. , 2013, ACS nano.

[83]  Imre Mäger,et al.  Extracellular vesicles: biology and emerging therapeutic opportunities , 2013, Nature Reviews Drug Discovery.

[84]  R. Schiffelers,et al.  Microvesicles and exosomes: opportunities for cell-derived membrane vesicles in drug delivery. , 2012, Journal of controlled release : official journal of the Controlled Release Society.

[85]  Per Sunnerhagen,et al.  Plasma exosomes can deliver exogenous short interfering RNA to monocytes and lymphocytes , 2012, Nucleic acids research.

[86]  Richard J Simpson,et al.  Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. , 2012, Methods.

[87]  M. Wood,et al.  Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes , 2011, Nature Biotechnology.

[88]  Dongmei Sun,et al.  A novel nanoparticle drug delivery system: the anti-inflammatory activity of curcumin is enhanced when encapsulated in exosomes. , 2010, Molecular therapy : the journal of the American Society of Gene Therapy.

[89]  T. Wurdinger,et al.  Microfluidic isolation and transcriptome analysis of serum microvesicles. , 2010, Lab on a chip.

[90]  J Ratajczak,et al.  Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery , 2006, Leukemia.

[91]  J. Le Pecq,et al.  Production and characterization of clinical grade exosomes derived from dendritic cells. , 2002, Journal of immunological methods.

[92]  C. Melief,et al.  B lymphocytes secrete antigen-presenting vesicles , 1996, The Journal of experimental medicine.

[93]  M. Adam,et al.  Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes , 1985, The Journal of cell biology.

[94]  E. Provasi,et al.  Methodologies for Scalable Production of High-Quality Purified Small Extracellular Vesicles from Conditioned Medium. , 2023, Methods in molecular biology.

[95]  Nagavendra Kommineni,et al.  Role of Exosomes for Delivery of Chemotherapeutic Drugs. , 2021, Critical reviews in therapeutic drug carrier systems.

[96]  Song Fan,et al.  Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy. , 2018, Biomaterials.

[97]  Gong Cheng,et al.  Aptamer-Conjugated Extracellular Nanovesicles for Targeted Drug Delivery. , 2018, Cancer research.