A Second Allele of eppA in Borrelia burgdorferi Strain B31 Is Located on the Previously Undetected Circular Plasmid cp9-2

ABSTRACT Although sequence analysis of Borrelia burgdorferiisolate B31 was recently declared “complete,” we found that cultures of this strain can contain a novel 9-kb circular plasmid, cp9-2. The newly described plasmid contains both sequence similarities with and differences from the previously identified B31 plasmid cp9-1 (formerly cp9). cp9-1 and cp9-2 each encode a unique allele of EppA, a putative membrane protein synthesized by B. burgdorferiduring mammalian infection.

[1]  B. Stevenson,et al.  Repetition, conservation, and variation: the multiple cp32 plasmids of Borrelia species. , 2000, Journal of molecular microbiology and biotechnology.

[2]  Brian Stevenson,et al.  The Relapsing Fever Spirochete Borrelia hermsiiContains Multiple, Antigen-Encoding Circular Plasmids That Are Homologous to the cp32 Plasmids of Lyme Disease Spirochetes , 2000, Infection and Immunity.

[3]  S. Casjens,et al.  Distribution of Twelve Linear Extrachromosomal DNAs in Natural Isolates of Lyme Disease Spirochetes , 2000, Journal of bacteriology.

[4]  B. Stevenson,et al.  Borrelia burgdorferi B31 Erp Proteins That Are Dominant Immunoblot Antigens of Animals Infected with Isolate B31 Are Recognized by Only a Subset of Human Lyme Disease Patient Sera , 2000, Journal of Clinical Microbiology.

[5]  O. White,et al.  A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi , 2000, Molecular microbiology.

[6]  T. Schwan,et al.  Temporal Changes in Outer Surface Proteins A and C of the Lyme Disease-Associated Spirochete, Borrelia burgdorferi, during the Chain of Infection in Ticks and Mice , 2000, Journal of Clinical Microbiology.

[7]  D. R. Blanco,et al.  Cloning and Molecular Characterization of Plasmid-Encoded Antigens of Borrelia burgdorferi , 1999, Infection and Immunity.

[8]  B. Stevenson,et al.  Stability of erp Loci duringBorrelia burgdorferi Infection: Recombination Is Not Required for Chronic Infection of Immunocompetent Mice , 1999, Infection and Immunity.

[9]  J. Radolf,et al.  Molecular and Evolutionary Analysis ofBorrelia burgdorferi 297 Circular Plasmid-Encoded Lipoproteins with OspE- and OspF-Like Leader Peptides , 1999, Infection and Immunity.

[10]  J. Bono,et al.  Borrelia burgdorferi Erp Proteins Are Immunogenic in Mammals Infected by Tick Bite, and Their Synthesis Is Inducible in Cultured Bacteria , 1998, Infection and Immunity.

[11]  S. Barthold,et al.  Humoral Immunity to Borrelia burgdorferiN40 Decorin Binding Proteins during Infection of Laboratory Mice , 1998, Infection and Immunity.

[12]  J. Bono,et al.  Oligopeptide permease in Borrelia burgdorferi: putative peptide-binding components encoded by both chromosomal and plasmid loci. , 1998, Microbiology.

[13]  J. Carlyon,et al.  Analysis of the Organization of Multicopy Linear- and Circular-Plasmid-Carried Open Reading Frames inBorrelia burgdorferi Sensu Lato Isolates , 1998, Infection and Immunity.

[14]  S. Salzberg,et al.  Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi , 1997, Nature.

[15]  J. Bono,et al.  The Borrelia burgdorferi circular plasmid cp26: conservation of plasmid structure and targeted inactivation of the ospC gene , 1997, Molecular microbiology.

[16]  J. Bono,et al.  Characterization of cp18, a naturally truncated member of the cp32 family of Borrelia burgdorferi plasmids , 1997, Journal of bacteriology.

[17]  S. Casjens,et al.  Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes , 1997, Journal of bacteriology.

[18]  S. Casjens,et al.  Directed insertion of a selectable marker into a circular plasmid of Borrelia burgdorferi , 1996, Journal of bacteriology.

[19]  R. C. Johnson,et al.  Correlation of plasmids with infectivity of Borrelia burgdorferi sensu stricto type strain B31 , 1996, Infection and immunity.

[20]  B. Stevenson,et al.  A family of genes located on four separate 32-kilobase circular plasmids in Borrelia burgdorferi B31 , 1996, Journal of bacteriology.

[21]  W. Zückert,et al.  Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element , 1996, Journal of bacteriology.

[22]  B. Kagan,et al.  Virulent strain associated outer membrane proteins of Borrelia burgdorferi. , 1995, The Journal of clinical investigation.

[23]  T. Schwan,et al.  Temperature-related differential expression of antigens in the Lyme disease spirochete, Borrelia burgdorferi , 1995, Infection and immunity.

[24]  R. C. Johnson,et al.  Analysis and comparison of plasmid profiles of Borrelia burgdorferi sensu lato strains , 1995, Journal of clinical microbiology.

[25]  M. Kramer,et al.  Molecular cloning and immunological characterization of a novel linear-plasmid-encoded gene, pG, of Borrelia burgdorferi expressed only in vivo , 1995, Infection and immunity.

[26]  S. Norris,et al.  High- and low-infectivity phenotypes of clonal populations of in vitro-cultured Borrelia burgdorferi , 1995, Infection and immunity.

[27]  T. Schwan,et al.  Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[28]  D. Haake,et al.  A 9.0-kilobase-pair circular plasmid of Borrelia burgdorferi encodes an exported protein: evidence for expression only during infection , 1994, Infection and immunity.

[29]  B. Luft,et al.  Complete nucleotide sequence of a circular plasmid from the Lyme disease spirochete, Borrelia burgdorferi , 1994, Journal of bacteriology.

[30]  Ruth R. Montgomery,et al.  Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease , 1994, Infection and immunity.

[31]  T. Schwan,et al.  Distribution and molecular analysis of Lyme disease spirochetes, Borrelia burgdorferi, isolated from ticks throughout California , 1993, Journal of clinical microbiology.

[32]  A. Barbour,et al.  The cryptic ospC gene of Borrelia burgdorferi B31 is located on a circular plasmid , 1993, Infection and immunity.

[33]  R. Marconi,et al.  Transcriptional analyses and mapping of the ospC gene in Lyme disease spirochetes , 1993, Journal of bacteriology.

[34]  C. Garon,et al.  Coumermycin A1 inhibits growth and induces relaxation of supercoiled plasmids in Borrelia burgdorferi, the Lyme disease agent , 1993, Antimicrobial Agents and Chemotherapy.

[35]  S. Norris,et al.  Low-passage-associated proteins of Borrelia burgdorferi B31: characterization and molecular cloning of OspD, a surface-exposed, plasmid-encoded lipoprotein , 1992, Infection and immunity.

[36]  S. Bergström,et al.  Extrachromosomal elements of spirochetes. , 1992, Research in microbiology.

[37]  L. Gern,et al.  Plasmid analysis and restriction fragment length polymorphisms of chromosomal DNA allow a distinction between Borrelia burgdorferi strains. , 1990, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[38]  T. Schwan,et al.  Borrelia burgdorferi contains repeated DNA sequences that are species specific and plasmid associated , 1990, Infection and immunity.

[39]  T. Schwan,et al.  Analysis of supercoiled circular plasmids in infectious and non-infectious Borrelia burgdorferi. , 1990, Microbial pathogenesis.

[40]  R. C. Johnson,et al.  Characterization of a circular plasmid from Borrelia burgdorferi, etiologic agent of Lyme disease , 1988, Journal of clinical microbiology.

[41]  A. Barbour,et al.  The Genes Encoding Major Surface Proteins of Borrelia burgdorferi Are Located on a Plasmid a , 1988, Annals of the New York Academy of Sciences.

[42]  T. Schwan,et al.  Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation , 1988, Infection and immunity.

[43]  A. Barbour,et al.  Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent , 1988, Journal of clinical microbiology.

[44]  R. C. Johnson,et al.  Colony formation and morphology in Borrelia burgdorferi , 1987, Journal of clinical microbiology.

[45]  G. Stanek.  Lyme disease and related disorders. , 1985, Microbiological sciences.

[46]  R. C. Johnson,et al.  Infection of Syrian hamsters with Lyme disease spirochetes , 1984, Journal of clinical microbiology.

[47]  A. Steigerwalt,et al.  Borrelia burgdorferi sp. nov.: Etiologic Agent of Lyme Disease , 1984 .

[48]  W. Burgdorfer,et al.  Lyme disease-a tick-borne spirochetosis? , 1983, Science.