[Study on improvement of cell affinity of polymer materials--modified poly(D,L-lactide) by anhydrous ammonia gaseous plasma].
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OBJECTIVE
To modify the surface of poly(D,L-lactide) film by anhydrous ammonia gaseous plasma treatment.
METHODS
The changes of contact angles were measured and surface energy were calculated. Mouse 3T3 fibroblast cells were cultured on plasma modified and control poly(D,L-lactide) films.
RESULTS
It was found that the hydrophilicity and surface energy of the materials have been increased after plasma treatment. Cell culture results showed that ammonia plasma treatment could promote the cell attachment and cells growth. After 4 days culture, the cells on the plasma treated films were 2-folds quantitatively compared with that of the control films.
CONCLUSION
Ammonia plasma treatment can improve the cell affinity to poly(D,L-lactide).