Extraction of lipids from microalgae by ultrasound application: prospection of the optimal extraction method.

Microalgae have the ability to grow rapidly, synthesize and accumulate large amounts (approximately 20-50% of dry weight) of lipids. A successful and economically viable algae based oil industry will depend on the selection of appropriate microalgal strains and the selection of the most suitable lipid extraction method. In this paper, five extraction methods were evaluated regarding the extraction of lipids from Chlorella vulgaris: Bligh and Dyer, Chen, Folch, Hara and Radin, and Soxhlet. Furthermore, the addition of silica powder was studied to evaluate the introduction of more shear stress to the system as to increase the disruption of cell walls. Among the studied methods, the Bligh and Dyer method assisted by ultrasound resulted in the highest extraction of oil from C. vulgaris (52.5% w/w). Addition of powder silica did not improve the extraction of oil.

[1]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[2]  F. Fernandes,et al.  Bioprospecting for oil producing microalgal strains: evaluation of oil and biomass production for ten microalgal strains. , 2011, Bioresource technology.

[3]  Vijayanand S. Moholkar,et al.  Mechanistic Assessment of Microalgal Lipid Extraction , 2010 .

[4]  H. Oh,et al.  Comparison of several methods for effective lipid extraction from microalgae. , 2010, Bioresource technology.

[5]  Timothy J. Mason,et al.  The uses of ultrasound in food technology , 1996 .

[6]  J. Benemann,et al.  Look Back at the U.S. Department of Energy's Aquatic Species Program: Biodiesel from Algae; Close-Out Report , 1998 .

[7]  K. G. Satyanarayana,et al.  A review on microalgae, a versatile source for sustainable energy and materials , 2011 .

[8]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[9]  Y. Bashan,et al.  Heterotrophic cultures of microalgae: metabolism and potential products. , 2011, Water research.

[10]  M. Demirbas Biofuels from algae for sustainable development , 2011 .

[11]  P. Prabakaran,et al.  A comparative study on effective cell disruption methods for lipid extraction from microalgae , 2011, Letters in applied microbiology.

[12]  Yusuf Chisti,et al.  Research review paper Biodiesel from microalgae , 2007 .

[13]  C. Howe,et al.  Biodiesel from algae: challenges and prospects. , 2010, Current opinion in biotechnology.

[14]  Y. Chisti Biodiesel from microalgae. , 2007, Biotechnology advances.

[15]  Michael R. Johns,et al.  Effect of C/N ratio and aeration on the fatty acid composition of heterotrophicChlorella sorokiniana , 1991, Journal of Applied Phycology.

[16]  N S Radin,et al.  Lipid extraction of tissues with a low-toxicity solvent. , 1978, Analytical biochemistry.

[17]  R. Guillard,et al.  Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. , 1962, Canadian journal of microbiology.

[18]  A. J. Sheppard,et al.  Comparison of methylene chloride and chloroform for the extraction of fats from food products , 1981 .

[19]  C. Posten,et al.  Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production , 2008, BioEnergy Research.

[20]  E. Ibáñez,et al.  Advanced analysis of nutraceuticals. , 2011, Journal of pharmaceutical and biomedical analysis.