Addressing a vascular endothelium array with blood components using underlying microfluidic channels.

Here, we show that an array of endothelial cells, addressable by an underlying microfluidic network of channels containing red blood cells, can be employed as an in vitro model of in vivo circulation to monitor cellular communication between different cell types in the drug discovery process.

[1]  G. Whitesides,et al.  Microfluidic arrays of fluid-fluid diffusional contacts as detection elements and combinatorial tools. , 2001, Analytical chemistry.

[2]  R. Zare,et al.  Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding. , 2005, Lab on a chip.

[3]  George M. Whitesides,et al.  Laminar flows: Subcellular positioning of small molecules , 2001, Nature.

[4]  J. Vacanti,et al.  Endothelialized Networks with a Vascular Geometry in Microfabricated Poly(dimethyl siloxane) , 2004 .

[5]  Yordan Kostov,et al.  The Design and Fabrication of Three‐Chamber Microscale Cell Culture Analog Devices with Integrated Dissolved Oxygen Sensors , 2008, Biotechnology progress.

[6]  A. Khademhosseini,et al.  Microscale technologies for tissue engineering and biology. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Dana M Spence,et al.  Deformation-induced release of ATP from erythrocytes in a poly(dimethylsiloxane)-based microchip with channels that mimic resistance vessels. , 2004, Analytical chemistry.

[8]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[9]  Michael G. Roper,et al.  Microfluidic chip for continuous monitoring of hormone secretion from live cells using an electrophoresis-based immunoassay. , 2003, Analytical chemistry.

[10]  Carl M. Cohen A path to improved pharmaceutical productivity , 2003, Nature Reviews Drug Discovery.

[11]  Jonathan V Sweedler,et al.  Nanofluidics and the role of nanocapillary array membranes in mass-limited chemical analysis. , 2006, The Analyst.

[12]  D. Spence,et al.  Fluorescence determination of nitric oxide production in stimulated and activated platelets. , 2007, Analytical chemistry.

[13]  Dana M Spence,et al.  Amperometric determination of nitric oxide derived from pulmonary artery endothelial cells immobilized in a microchip channel. , 2004, The Analyst.

[14]  K. Audus,et al.  The Application of Bovine Brain Microvessel Endothelial-Cell Monolayers Grown onto Polycarbonate Membranes in Vitro to Estimate the Potential Permeability of Solutes Through the Blood–Brain Barrier , 1989, Pharmaceutical Research.

[15]  Luke P. Lee,et al.  Microfluidics-based systems biology. , 2006, Molecular bioSystems.

[16]  A. Manz,et al.  Lab-on-a-chip: microfluidics in drug discovery , 2006, Nature Reviews Drug Discovery.

[17]  Dana M. Spence,et al.  A Microchip‐Based System for Immobilizing PC 12 Cells and Amperometrically Detecting Catecholamines Released After Stimulation with Calcium , 2005 .

[18]  C. Henry,et al.  Dual-electrode electrochemical detection for poly(dimethylsiloxane)-fabricated capillary electrophoresis microchips. , 2000, Analytical chemistry.