Three-dimensional computer reconstruction and display of neuronal structure.

Shaded, three-dimensional images of microscopic neuronal structures are generated using a special computer graphics system known as THREAD (Three-Dimensional Reconstruction Aand Display). This system uses both a computer and its associated image processing hard-ware and specialized computer software to produce both line-drawings (contour-grams) and shaded images obtained from 35 mm film negatives of cross-sectional images of microscopic neuronal structures. The resulting image is displayed on a TV monitor in either color or black and white and can be rotated to view all surfaces. Structural volume and planar surface areas are also evaluated during the procedure. Examples of a brainstem region and of a neuron nucleolus are presented.

[1]  J C Mazziotta,et al.  A device for the precise transfer of serial sections for electron microscopy. , 1973, Stain technology.

[2]  CYRUS LEVINTHAL,et al.  Three Dimensional Reconstruction from Serial Sections , 1972, Nature.

[3]  F. Sjöstrand.  Ultrastructure of retinal rod synapses of the guinea pig eye as revealed by three-dimensional reconstructions from serial sections. , 1958, Journal of ultrastructure research.

[4]  Edward P. Sayre,et al.  Computer-aided three-dimensional reconstruction and quantitative analysis of cells from serial electron microscopic montages of foetal monkey brain , 1974, Nature.

[5]  H C Mitchell,et al.  Three Dimensions in Fine Structure , 1965, Science.

[6]  Raphael Poritsky,et al.  Two and three dimensional ultrastructure of boutons and glial cells on the motoneuronal surface in the cat spinal cord , 1969 .

[7]  R L Schultz,et al.  Intranuclear inclusions in neurons of the cat primary olfactory system. , 1971, Brain research.

[8]  H. K. Huang,et al.  THREAD (three-dimensional reconstruction and display) with biomedical applications in neuron ultrastructure and computerized tomography , 1976, AFIPS '76.

[9]  W. M. Cowan,et al.  A computer system for the measurement of cell and nuclear sizes* , 1973, Journal of microscopy.

[10]  Robert S. Ledley,et al.  FIDAC - Film input to digital automatic computer , 1971, Pattern Recognit..

[11]  F M Keddie,et al.  Three-dimensional reconstruction of Pityrosporum yeast cells based on serial section electron microscopy. , 1969, Journal of ultrastructure research.

[12]  U Karlsson,et al.  Three-dimensional studies of neurons in the lateral geniculate nucleus of the rat. I. Organelle organization in the perikaryon and its proximal branches. , 1966, Journal of ultrastructure research.

[13]  D F Wann,et al.  An on-line digital-computer system for the semiautomatic analysis of Golgi-impregnated neurons. , 1973, IEEE transactions on bio-medical engineering.

[14]  L Barajas,et al.  The ultrastructure of the juxtaglomerular apparatus as disclosed by three-dimensional reconstructions from serial sections. The anatomical relationship between the tubular and vascular components. , 1970, Journal of ultrastructure research.

[15]  C. Levinthal,et al.  Structure and development of neuronal connections in isogenic organisms: variations and similarities in the optic system of Daphnia magna. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[16]  B H BANG,et al.  Graphic reconstruction of the third dimension from serial electron microphotographs. , 1957, Journal of ultrastructure research.

[17]  HENRI GOURAUD,et al.  Continuous Shading of Curved Surfaces , 1971, IEEE Transactions on Computers.

[18]  P. Rakić.  Mode of cell migration to the superficial layers of fetal monkey neocortex , 1972, The Journal of comparative neurology.

[19]  T J Willey,et al.  Computer graphics in three dimensions for perspective reconstruction of brain ultrastructure. , 1973, IEEE transactions on bio-medical engineering.

[20]  Robert F. Dunn.  Graphic three‐dimensional representations from serial sections * , 1972 .

[21]  U Karlsson,et al.  Three-dimensional studies of neurons in the lateral geniculate nucleus of the rat. II. Environment of perikarya and proximal parts of their branches. , 1966, Journal of ultrastructure research.