Understanding the toxicity of carbon nanotubes in the environment is crucial to the control of nanomaterials in producing and processing and the assessment of health risk for human: a review.

With the development and application of carbon nanotubes (CNTs), the potential hazards of CNTs to biological systems and the environment are getting more and more attention. This review evaluated the effects of physicochemical properties of CNTs on toxicity and summarized the advances on the mechanism of CNTs toxicity. We also proposed the possible hazards associated with CNTs and harmful effects resulting from exposure of aquatic animals, bacteria and higher plants to CNTs in vitro and in vivo. The current knowledge and gaps on CNTs were outlined as a potential problem for the environment and human health. The current research gaps on CNTs toxicity were identified and the further studying focus was proposed, too. This essay concluded with a set of recommendations for the advancement of understanding of the role of CNTs and future challenges in environmental and ecotoxicological research.

[1]  Menachem Elimelech,et al.  Single-walled carbon nanotubes exhibit strong antimicrobial activity. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[2]  Jamie R Lead,et al.  Manufactured nanoparticles: an overview of their chemistry, interactions and potential environmental implications. , 2008, The Science of the total environment.

[3]  B. Deng,et al.  Toxicity of carbon nanotubes to freshwater aquatic invertebrates , 2012, Environmental toxicology and chemistry.

[4]  R. Weisman,et al.  Single-walled carbon nanotubes in the intact organism: near-IR imaging and biocompatibility studies in Drosophila. , 2007, Nano letters.

[5]  C. Redon,et al.  Intercellular communication of cellular stress monitored by gamma-H2AX induction. , 2009, Carcinogenesis.

[6]  Huajian Gao,et al.  Effect of single wall carbon nanotubes on human HEK293 cells. , 2005, Toxicology letters.

[7]  V. Castranova,et al.  Direct and indirect effects of single walled carbon nanotubes on RAW 264.7 macrophages: role of iron. , 2006, Toxicology letters.

[8]  Jürgen Pauluhn,et al.  Subchronic 13-week inhalation exposure of rats to multiwalled carbon nanotubes: toxic effects are determined by density of agglomerate structures, not fibrillar structures. , 2010, Toxicological sciences : an official journal of the Society of Toxicology.

[9]  Á. Jos,et al.  Oxidative stress responses to carboxylic acid functionalized single wall carbon nanotubes on the human intestinal cell line Caco-2. , 2012, Toxicology in vitro : an international journal published in association with BIBRA.

[10]  Elijah J Petersen,et al.  Biological uptake and depuration of carbon nanotubes by Daphnia magna. , 2009, Environmental science & technology.

[11]  Peter Wick,et al.  Reviewing the environmental and human health knowledge base of carbon nanotubes. , 2007, Ciencia & saude coletiva.

[12]  J. West,et al.  Nano-C60 cytotoxicity is due to lipid peroxidation. , 2005, Biomaterials.

[13]  Patrick A. Cooke,et al.  Molecular Characterization of the Cytotoxic Mechanism of Multiwall Carbon Nanotubes and Nano-onions on Human Skin Fibroblast , 2005 .

[14]  W. Stark,et al.  The degree and kind of agglomeration affect carbon nanotube cytotoxicity. , 2007, Toxicology letters.

[15]  J. James,et al.  Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[16]  Philip Ball,et al.  Roll up for the revolution , 2001, Nature.

[17]  M L Kashon,et al.  Induction of aneuploidy by single‐walled carbon nanotubes , 2009, Environmental and molecular mutagenesis.

[18]  Kun Yang,et al.  Systematic and quantitative investigation of the mechanism of carbon nanotubes' toxicity toward algae. , 2012, Environmental science & technology.

[19]  E. Eisen,et al.  Epidemiologic Challenges for Studies of Occupational Exposure to Engineered Nanoparticles; A Commentary , 2011, Journal of occupational and environmental medicine.

[20]  Shuk Han Cheng,et al.  Effect of carbon nanotubes on developing zebrafish (Danio Rerio) embryos , 2007, Environmental toxicology and chemistry.

[21]  Alok Dhawan,et al.  Toxicity assessment of nanomaterials: methods and challenges , 2010, Analytical and bioanalytical chemistry.

[22]  Parag Aggarwal,et al.  Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacy. , 2009, Advanced drug delivery reviews.

[23]  N. Wu,et al.  Particle length-dependent titanium dioxide nanomaterials toxicity and bioactivity , 2009, Particle and Fibre Toxicology.

[24]  M. Morandi,et al.  Nanoparticle‐induced platelet aggregation and vascular thrombosis , 2005, British journal of pharmacology.

[25]  Yuan Yuan,et al.  Long-circulation of hemoglobin-loaded polymeric nanoparticles as oxygen carriers with modulated surface charges. , 2009, International journal of pharmaceutics.

[26]  M. Elimelech,et al.  Toxic effects of single-walled carbon nanotubes in the development of E. coli biofilm. , 2010, Environmental science & technology.

[27]  Sophie Lanone,et al.  Biomedical applications and potential health risks of nanomaterials: molecular mechanisms. , 2006, Current molecular medicine.

[28]  S. Fukushima,et al.  Genotoxicity and Cytotoxicity of Multi‐wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers , 2010, Journal of occupational health.

[29]  E. Petersen,et al.  Influence of polyethyleneimine graftings of multi-walled carbon nanotubes on their accumulation and elimination by and toxicity to Daphnia magna. , 2011, Environmental science & technology.

[30]  B. Halliwell,et al.  Free radicals in biology and medicine , 1985 .

[31]  Chen Liqiang,et al.  Toxicity of graphene oxide and multi-walled carbon nanotubes against human cells and zebrafish , 2012 .

[32]  J. Kreuter Nanoparticles--a historical perspective. , 2007, International journal of pharmaceutics.

[33]  Y. Kim,et al.  In vivo immunological toxicity in mice of carbon nanotubes with impurities , 2009 .

[34]  K. Donaldson,et al.  Signs of stress , 2006, Nature nanotechnology.

[35]  Xinyuan Liu,et al.  Differential toxicity of carbon nanomaterials in Drosophila: larval dietary uptake is benign, but adult exposure causes locomotor impairment and mortality. , 2009, Environmental science & technology.

[36]  Zafar Iqbal,et al.  Single-walled Carbon Nanotubes Are a New Class of Ion Channel Blockers* , 2003, Journal of Biological Chemistry.

[37]  Julie W. Fitzpatrick,et al.  Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy , 2005, Particle and Fibre Toxicology.

[38]  V. Himabindu,et al.  Pulmonary toxicity assessment of multiwalled carbon nanotubes in rats following intratracheal instillation , 2012, Environmental toxicology.

[39]  Vincent Castranova,et al.  Single-walled Carbon Nanotubes: Geno- and Cytotoxic Effects in Lung Fibroblast V79 Cells , 2007, Journal of toxicology and environmental health. Part A.

[40]  Chia-Liang Cheng,et al.  Biocompatible and detectable carboxylated nanodiamond on human cell , 2007 .

[41]  G. Ramesh,et al.  Multiwalled carbon nanotubes activate NF-κB and AP-1 signaling pathways to induce apoptosis in rat lung epithelial cells , 2010, Apoptosis.

[42]  Brian D Holt,et al.  Carbon nanotubes reorganize actin structures in cells and ex vivo. , 2010, ACS nano.

[43]  Scott W Burchiel,et al.  Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes. , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[44]  E. Menaszek,et al.  Comparative in vivo biocompatibility study of single- and multi-wall carbon nanotubes. , 2008, Acta biomaterialia.

[45]  Paul R. Lockman,et al.  Nanoparticle Surface Charges Alter Blood–Brain Barrier Integrity and Permeability , 2004, Journal of drug targeting.

[46]  H. Krug,et al.  Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants. , 2007, Toxicology letters.

[47]  M. Maugey,et al.  Hot-drawing of single and multiwall carbon nanotube fibers for high toughness and alignment. , 2005, Nano letters.

[48]  Yang Xu,et al.  Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. , 2009, ACS nano.

[49]  H. Attar,et al.  Study on toxicity of manufactured nanoparticles to bacteria Vibrio fischeri using homemade luminometer , 2022 .

[50]  Eva Oberdörster,et al.  Ecotoxicology of carbon-based engineered nanoparticles: Effects of fullerene (C60) on aquatic organisms , 2006 .

[51]  Helinor J Johnston,et al.  A critical review of the biological mechanisms underlying the in vivo and in vitro toxicity of carbon nanotubes: The contribution of physico-chemical characteristics , 2010, Nanotoxicology.

[52]  M. Kirsch‐Volders,et al.  Adaptations of the in vitro MN assay for the genotoxicity assessment of nanomaterials. , 2011, Mutagenesis.

[53]  Toxicity Study of Two Different Synthesized Silver Nanoparticles on Bacteria Vibrio Fischeri , 2012 .

[54]  Andrew D Maynard,et al.  The new toxicology of sophisticated materials: nanotoxicology and beyond. , 2011, Toxicological sciences : an official journal of the Society of Toxicology.

[55]  T. Xia,et al.  Understanding biophysicochemical interactions at the nano-bio interface. , 2009, Nature materials.

[56]  Haifang Wang,et al.  Long-term accumulation and low toxicity of single-walled carbon nanotubes in intravenously exposed mice. , 2008, Toxicology letters.

[57]  A. Harris,et al.  Multiwalled carbon nanotubes in alfalfa and wheat: toxicology and uptake , 2012, Journal of The Royal Society Interface.

[58]  Seishiro Hirano,et al.  Multi-walled carbon nanotubes injure the plasma membrane of macrophages. , 2008, Toxicology and applied pharmacology.

[59]  H. Schwarz,et al.  Cytotoxicity of single-wall carbon nanotubes on human fibroblasts. , 2006, Toxicology in vitro : an international journal published in association with BIBRA.

[60]  D. V. Kuznetsov,et al.  Acute toxic and cytogenetic effects of carbon nanotubes on aquatic organisms and bacteria , 2012, Nanotechnologies in Russia.

[61]  P. Baron,et al.  Exposure to Carbon Nanotube Material: Aerosol Release During the Handling of Unrefined Single-Walled Carbon Nanotube Material , 2004, Journal of toxicology and environmental health. Part A.

[62]  M. Prato,et al.  Carbon nanotubes as nanomedicines: from toxicology to pharmacology. , 2006, Advanced drug delivery reviews.

[63]  B. van Ravenzwaay,et al.  Inhalation toxicity of multiwall carbon nanotubes in rats exposed for 3 months. , 2009, Toxicological sciences : an official journal of the Society of Toxicology.

[64]  W. Mello,et al.  Intercellular Communication , 1977, Springer US.

[65]  L. Forró,et al.  Cellular toxicity of carbon-based nanomaterials. , 2006, Nano letters.

[66]  Jayanth Panyam,et al.  Polymer degradation and in vitro release of a model protein from poly(D,L-lactide-co-glycolide) nano- and microparticles. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[67]  X. He,et al.  Multiwalled carbon nanotubes induce a fibrogenic response by stimulating reactive oxygen species production, activating NF-κB signaling, and promoting fibroblast-to-myofibroblast transformation. , 2011, Chemical research in toxicology.

[68]  Peter Wick,et al.  Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells , 2013 .

[69]  N. Monteiro-Riviere,et al.  Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators. , 2007, Nano letters.

[70]  B. Lich,et al.  Imaging methods for determining uptake and toxicity of carbon nanotubes in vitro and in vivo. , 2011, Nanomedicine.

[71]  Julian Moger,et al.  Sublethal toxicity of nano-titanium dioxide and carbon nanotubes in a sediment dwelling marine polychaete. , 2010, Environmental pollution.

[72]  Ryan C. Templeton,et al.  Life-cycle effects of single-walled carbon nanotubes (SWNTs) on an estuarine meiobenthic copepod. , 2006, Environmental science & technology.

[73]  Craig A. Poland,et al.  Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study. , 2008, Nature nanotechnology.

[74]  M. Ramezani,et al.  Alkylcarboxylate grafting to polyethylenimine: a simple approach to producing a DNA nanocarrier with low toxicity , 2009, The journal of gene medicine.

[75]  Shuk Han Cheng,et al.  Influence of carbon nanotube length on toxicity to zebrafish embryos , 2012, International journal of nanomedicine.

[76]  Eleonore Fröhlich,et al.  The role of nanoparticle size in hemocompatibility. , 2009, Toxicology.

[77]  R. M. Adibhatla,et al.  Lipid oxidation and peroxidation in CNS health and disease: from molecular mechanisms to therapeutic opportunities. , 2010, Antioxidants & redox signaling.

[78]  N. Bottini,et al.  Multi-walled carbon nanotubes induce T lymphocyte apoptosis. , 2006, Toxicology letters.

[79]  Naomi K Fukagawa,et al.  Assessing nanotoxicity in cells in vitro. , 2010, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[80]  G. Oberdörster,et al.  Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles , 2005, Environmental health perspectives.

[81]  X. Tang,et al.  Impact of carbon nanotubes on the ingestion and digestion of bacteria by ciliated protozoa. , 2008, Nature nanotechnology.

[82]  Pratim Biswas,et al.  Does nanoparticle activity depend upon size and crystal phase? , 2008, Nanotoxicology.

[83]  Xizhong Shen,et al.  The hepatotoxicity of multi-walled carbon nanotubes in mice , 2009, Nanotechnology.

[84]  K. Griendling,et al.  Basic mechanisms of oxidative stress and reactive oxygen species in cardiovascular injury. , 2007, Trends in cardiovascular medicine.

[85]  Ruhong Zhou,et al.  Plugging into proteins: poisoning protein function by a hydrophobic nanoparticle. , 2010, ACS nano.

[86]  M. Hussain,et al.  β-Galactosidase Leakage from Escherichia coli Points to Mechanical Damageas Likely Cause of Carbon Nanotube Toxicity , 2012 .

[87]  Wolfgang Kreyling,et al.  Ultrafine Particles Cross Cellular Membranes by Nonphagocytic Mechanisms in Lungs and in Cultured Cells , 2005, Environmental health perspectives.

[88]  Jin-Ho Choy,et al.  Toxicological effects of inorganic nanoparticles on human lung cancer A549 cells. , 2009, Journal of inorganic biochemistry.

[89]  Emmanuel Flahaut,et al.  Carbon nanotubes induce inflammation but decrease the production of reactive oxygen species in lung. , 2010, Toxicology.

[90]  V. Hartenstein,et al.  Drosophila melanogaster , 2005 .

[91]  P. Baron,et al.  Unusual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice. , 2005, American journal of physiology. Lung cellular and molecular physiology.

[92]  J. Mcgee,et al.  Enhanced in vitro and in vivo toxicity of poly-dispersed acid-functionalized single-wall carbon nanotubes , 2007 .

[93]  François Huaux,et al.  Respiratory toxicity of carbon nanotubes: How worried should we be? , 2006 .

[94]  V. Castranova,et al.  Alteration of deposition pattern and pulmonary response as a result of improved dispersion of aspirated single-walled carbon nanotubes in a mouse model. , 2008, American journal of physiology. Lung cellular and molecular physiology.

[95]  Menachem Elimelech,et al.  Physicochemical determinants of multiwalled carbon nanotube bacterial cytotoxicity. , 2008, Environmental science & technology.

[96]  J. I. Phillips,et al.  Pulmonary and systemic toxicity following exposure to nickel nanoparticles. , 2010, American journal of industrial medicine.

[97]  Yi Zhang,et al.  Repeated carbon nanotube administrations in male mice cause reversible testis damage without affecting fertility , 2010, Nature Nanotechnology.

[98]  Robert L. Tanguay,et al.  In vivo biodistribution and toxicity depends on nanomaterial composition, size, surface functionalisation and route of exposure , 2008 .

[99]  Richard D Handy,et al.  Toxicity of single walled carbon nanotubes to rainbow trout, (Oncorhynchus mykiss): respiratory toxicity, organ pathologies, and other physiological effects. , 2007, Aquatic toxicology.

[100]  Peter Wick,et al.  Effects of carbon nanotubes on primary neurons and glial cells. , 2009, Neurotoxicology.

[101]  T. Ichihashi,et al.  Single-shell carbon nanotubes of 1-nm diameter , 1993, Nature.

[102]  Zhuang Liu,et al.  Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway. , 2006, Angewandte Chemie.

[103]  T. Webb,et al.  Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[104]  Walter J. Weber,et al.  Ecological Uptake and Depuration of Carbon Nanotubes by Lumbriculus variegatus , 2008, Environmental health perspectives.

[105]  Saura C. Sahu,et al.  Nanotoxicity: From In Vivo and In Vitro Models to Health Risks , 2009 .

[106]  Emmanuel Flahaut,et al.  Assessment of the potential in vivo ecotoxicity of Double-Walled Carbon Nanotubes (DWNTs) in water, using the amphibian Ambystoma mexicanum , 2007 .

[107]  Morteza Mahmoudi,et al.  Toxicity of Nanomaterials , 2012 .

[108]  Yuliang Zhao,et al.  Cytotoxicity of carbon nanomaterials: single-wall nanotube, multi-wall nanotube, and fullerene. , 2005, Environmental science & technology.

[109]  Y. Hung,et al.  Assessment of the In Vivo Toxicity of Gold Nanoparticles , 2009, Nanoscale research letters.

[110]  Vincent M Rotello,et al.  Toxicity of gold nanoparticles functionalized with cationic and anionic side chains. , 2004, Bioconjugate chemistry.

[111]  Scott C. Brown,et al.  Research strategies for safety evaluation of nanomaterials. Part VI. Characterization of nanoscale particles for toxicological evaluation. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.

[112]  Silvana Andreescu,et al.  Toxicity and developmental defects of different sizes and shape nickel nanoparticles in zebrafish. , 2009, Environmental science & technology.

[113]  Qing Huang,et al.  Effects of serum proteins on intracellular uptake and cytotoxicity of carbon nanoparticles , 2009 .

[114]  A. Alam,et al.  Carbon nanotubes: a review on future generation nano device , 2014 .

[115]  Masato Yasuhara,et al.  Physicochemical Properties and Cellular Toxicity of Nanocrystal Quantum Dots Depend on Their Surface Modification , 2004 .

[116]  M. Elimelech,et al.  Impact of surface functionalization on bacterial cytotoxicity of single-walled carbon nanotubes. , 2012, Environmental science & technology.

[117]  Hamidreza Ghandehari,et al.  Impact of silica nanoparticle design on cellular toxicity and hemolytic activity. , 2011, ACS nano.

[118]  Francesco Stellacci,et al.  Effect of surface properties on nanoparticle-cell interactions. , 2010, Small.

[119]  H. Karlsson,et al.  The comet assay in nanotoxicology research , 2010, Analytical and bioanalytical chemistry.

[120]  P. Midgley,et al.  Four-dimensional spectral tomography of carbonaceous nanocomposites. , 2006, Nano letters.

[121]  Yoshikazu Matsuda,et al.  Proteomics-based safety evaluation of multi-walled carbon nanotubes. , 2010, Toxicology and applied pharmacology.

[122]  A. Huczko,et al.  PHYSIOLOGICAL TESTING OF CARBON NANOTUBES: ARE THEY ASBESTOS-LIKE? , 2001 .

[123]  S. Bellucci,et al.  Comparative cyto-genotoxicity assessment of functionalized and pristine multiwalled carbon nanotubes on human lung epithelial cells. , 2012, Toxicology in vitro : an international journal published in association with BIBRA.

[124]  J. Nagy,et al.  Respiratory toxicity of multi-wall carbon nanotubes. , 2005, Toxicology and applied pharmacology.

[125]  Rui Qiao,et al.  In vivo biomodification of lipid-coated carbon nanotubes by Daphnia magna. , 2007, Environmental science & technology.

[126]  Richard D Handy,et al.  Dietary toxicity of single-walled carbon nanotubes and fullerenes (C60) in rainbow trout (Oncorhynchus mykiss) , 2011, Nanotoxicology.

[127]  S. Iijima Helical microtubules of graphitic carbon , 1991, Nature.

[128]  Sandro Santucci,et al.  Effects of single and multi walled carbon nanotubes on macrophages: cyto and genotoxicity and electron microscopy. , 2011, Mutation research.

[129]  Jing Xu,et al.  In vitro toxicity of multi-walled carbon nanotubes in C6 rat glioma cells. , 2012, Neurotoxicology.

[130]  Xiaoshan Zhu,et al.  Acute toxicities of six manufactured nanomaterial suspensions to Daphnia magna , 2009 .

[131]  Tim Liedl,et al.  Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles. , 2005, Nano letters.

[132]  J. Hong,et al.  A single intratracheal instillation of single-walled carbon nanotubes induced early lung fibrosis and subchronic tissue damage in mice , 2011, Archives of Toxicology.

[133]  M. Kalbáčová,et al.  Influence of single-walled carbon nanotube films on metabolic activity and adherence of human osteoblasts , 2007 .

[134]  M. Terrones,et al.  Biocompatibility and toxicological studies of carbon nanotubes doped with nitrogen. , 2006, Nano letters.

[135]  N. Chandrasekaran,et al.  Antimicrobial sensitivity of Escherichia coli to alumina nanoparticles. , 2009, Nanomedicine : nanotechnology, biology, and medicine.

[136]  M. Prato,et al.  Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type. , 2007, Nature nanotechnology.

[137]  N. Herlin‐Boime,et al.  In vitro investigation of oxide nanoparticle and carbon nanotube toxicity and intracellular accumulation in A549 human pneumocytes. , 2008, Toxicology.

[138]  C. Mikoryak,et al.  Single-walled carbon nanotube interactions with HeLa cells , 2007, Journal of nanobiotechnology.

[139]  B. Lehnert,et al.  Correlation between particle size, in vivo particle persistence, and lung injury. , 1994, Environmental health perspectives.

[140]  Liping Wei,et al.  Cytotoxicity effects of water dispersible oxidized multiwalled carbon nanotubes on marine alga, Dunaliella tertiolecta. , 2010, Aquatic toxicology.

[141]  R. Aitken,et al.  Carbon nanotubes: a review of their properties in relation to pulmonary toxicology and workplace safety. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.