Measurement of agonist efficacy using an α2A‐adrenoceptor‐Gi1α fusion protein

[1]  G. Milligan,et al.  Rescue of Functional Interactions between the α2A-Adrenoreceptor and Acylation-resistant Forms of Gi1α by Expressing the Proteins from Chimeric Open Reading Frames* , 1997, The Journal of Biological Chemistry.

[2]  G. Milligan,et al.  Measurement of agonist-induced guanine nucleotide turnover by the G-protein Gi1alpha when constrained within an alpha2A-adrenoceptor-Gi1alpha fusion protein. , 1997, The Biochemical journal.

[3]  M. Scheinin,et al.  Gene targeting — homing in on α2-adrenoceptor-subtype function , 1997 .

[4]  S. Rees,et al.  Interactions of the alpha2A-adrenoceptor with multiple Gi-family G-proteins: studies with pertussis toxin-resistant G-protein mutants. , 1997, The Biochemical journal.

[5]  G. Milligan,et al.  Agonist regulation of adenylate cyclase activity in neuroblastoma x glioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the beta 2-adrenoceptor. Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity. , 1996, The Biochemical journal.

[6]  G. Milligan,et al.  Analysis of inverse agonism at the delta opioid receptor after expression in Rat 1 fibroblasts. , 1996, The Biochemical journal.

[7]  R. Graham,et al.  Constitutive activation of a single effector pathway: evidence for multiple activation states of a G protein-coupled receptor. , 1996, Molecular pharmacology.

[8]  G. Guillon,et al.  Close association of the alpha subunits of Gq and G11 G proteins with actin filaments in WRK1 cells: relation to G protein-mediated phospholipase C activation. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[9]  G. Milligan,et al.  Analysis of the role of receptor number in defining the intrinsic activity and potency of partial agonists in neuroblastoma x glioma hybrid NG108-15 cells transfected to express differing levels of the human beta 2-adrenoceptor. , 1995, Molecular pharmacology.

[10]  T. Kenakin,et al.  Agonist-receptor efficacy. II. Agonist trafficking of receptor signals. , 1995, Trends in pharmacological sciences.

[11]  T. Kenakin,et al.  Agonist-receptor efficacy. I: Mechanisms of efficacy and receptor promiscuity. , 1995, Trends in pharmacological sciences.

[12]  R. Neubig Membrane organization in G‐protein mechanisms , 1994, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[13]  M. Spedding,et al.  Alpha 2-adrenoceptors: more subtypes but fewer functional differences. , 1994, Trends in pharmacological sciences.

[14]  S. Liggett,et al.  Contribution of ligand structure to activation of alpha 2-adrenergic receptor subtype coupling to Gs. , 1994, Molecular pharmacology.

[15]  P. Evans,et al.  Agonist‐specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems. , 1994, The EMBO journal.

[16]  T. Wieland,et al.  Measurement of receptor-stimulated guanosine 5'-O-(gamma-thio)triphosphate binding by G proteins. , 1994, Methods in enzymology.

[17]  Laurent Journot,et al.  Differential signal transduction by five splice variants of the PACAP receptor , 1993, Nature.

[18]  D. Hoyer,et al.  Partial agonists, full agonists, antagonists: dilemmas of definition. , 1993, Trends in pharmacological sciences.

[19]  G. Milligan,et al.  Mechanisms of multifunctional signalling by G protein-linked receptors. , 1993, Trends in pharmacological sciences.

[20]  Y. Okuma,et al.  Immunoprecipitation of alpha 2a-adrenergic receptor-GTP-binding protein complexes using GTP-binding protein selective antisera. Changes in receptor/GTP-binding protein interaction following agonist binding. , 1992, The Journal of biological chemistry.

[21]  C. A. Guyer,et al.  Cloning, sequencing, and expression of the gene encoding the porcine alpha 2-adrenergic receptor. Allosteric modulation by Na+, H+, and amiloride analogs. , 1990, The Journal of biological chemistry.

[22]  M. Rasenick,et al.  Tubulin binds specifically to the signal-transducing proteins, Gs alpha and Gi alpha 1. , 1990, The Journal of biological chemistry.

[23]  T. Kenakin Challenges for receptor theory as a tool for drug and drug receptor classification. , 1989, Trends in pharmacological sciences.

[24]  G. Milligan,et al.  Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins. , 1988, The Biochemical journal.

[25]  G. Koski,et al.  Opiates inhibit adenylate cyclase by stimulating GTP hydrolysis. , 1981, Proceedings of the National Academy of Sciences of the United States of America.