Inhibition of influenza virus replication by -amanitin: mode of action.

The replication of influenza virus in chick embryo fibroblast cells is inhibited by alpha-amanitin added during the first 2 hr of infection at concentrations similar to those required to inhibit cellular DNA-dependent RNA polymerase form II in vivo. Of two periods of increased RNA synthesis observed in cells infected with influenza virus, only the first, occurring from 0 to 2 hr after infection, is sensitive to alpha-amanitin. During this early period, there is a stimulation of the activity of DNA-dependent RNA polymerase II of nuclei isolated from infected cells. The data suggest that DNA transcription mediated by polymerase II is essential for influenza virus replication.

[1]  R. F. Cox,et al.  Transcription of mammalian chromatin by mammalian DNA-dependent RNA polymerases. , 1971, European journal of biochemistry.

[2]  S. Penman,et al.  Products of RNA polymerases in HeLa cell nuclei. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[3]  E. Penhoet,et al.  RNA-dependent RNA polymerase activity in influenza virions. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[4]  P. Butterworth,et al.  Studies on the origin of the form Ib mammalian DNA‐dependent RNA polymerase , 1971, FEBS letters.

[5]  H. Klenk,et al.  Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. , 1970, Virology.

[6]  I. Schulze.  The structure of influenza virus. I. The polypeptides of the virion. , 1970, Virology.

[7]  C. Scholtissek,et al.  Specific Inhibition of Influenza Replication by α-Amanitin , 1970, Nature.

[8]  B. Mahy,et al.  In Vitro Product of a Ribonucleic Acid Polymerase Induced by Influenza Virus , 1970, Journal of virology.

[9]  B. Mahy,et al.  Ribonucleic Acid Polymerase Induced in Cells Infected with Sendai Virus , 1970, Journal of virology.

[10]  L. Fiume,et al.  Amantis. Chemistry and action , 1970, FEBS letters.

[11]  F. Stirpe,et al.  Simultaneous assay of RNA polymerase I and II in nuclei isolated from resting and growing rat liver with the use of α‐amanitin , 1970, FEBS letters.

[12]  W. Rutter,et al.  Specific nucleolar and nucleoplasmic RNA polymerases. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Y. Ghendon,et al.  The fate of influenza virus RNA in cells treated with ultraviolet rays. , 1970, Journal of General Virology.

[14]  P. Chambon,et al.  α-Amanitin: A specific inhibitor of one of two DNA-dependent RNA polymerase activities from calf thymus , 1970 .

[15]  W. Rutter,et al.  Multiple Forms of DNA-dependent RNA Polymerase in Eukaryotic Organisms , 1969, Nature.

[16]  N. Dimmock.  New virus-specific antigens in cells infected with influenza virus. , 1969, Virology.

[17]  B. Mahy,et al.  Deoxyribonucleic Acid-dependent Ribonucleic Acid Polymerase Activity in Cells Infected with Influenza Virus , 1968, Journal of virology.

[18]  D. Nayak,et al.  Influence of mitomycin C on the replication of influenza viruses. , 1966, Virology.

[19]  J. Bader.  THE REQUIREMENT FOR DNA SYNTHESIS IN THE GROWTH OF ROUS SARCOMA AND ROUS-ASSOCIATED VIRUSES. , 1965, Virology.

[20]  D. O. White,et al.  DELAY IN THE MULTIPLICATION OF INFLUENZA VIRUS. , 1965, Virology.

[21]  R. D. Barry.  THE EFFECTS OF ACTINOMYCIN D AND ULTRAVIOLET IRRADIATION ON THE PRODUCTION OF FOWL PLAGUE VIRUS. , 1964, Virology.

[22]  D. Buthala,et al.  Cell Culture Studies on Antiviral Agents , 1964, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[23]  R. D. Barry,et al.  Participation of Deoxyribonucleic Acid in the Multiplication of Influenza Virus , 1962, Nature.

[24]  J. Chauveau,et al.  Isolation of pure and unaltered liver nuclei morphology and biochemical composition. , 1956, Experimental cell research.