Effect of radio-frequency electromagnetic radiations (RF-EMR) on passive avoidance behaviour and hippocampal morphology in Wistar rats

Abstract Introduction. The interaction of mobile phone radio-frequency electromagnetic radiation (RF-EMR) with the brain is a serious concern of our society. Objective. We evaluated the effect of RF-EMR from mobile phones on passive avoidance behaviour and hippocampal morphology in rats. Materials and methods. Healthy male albino Wistar rats were exposed to RF-EMR by giving 50 missed calls (within 1 hour) per day for 4 weeks, keeping a GSM (0.9 GHz/1.8 GHz) mobile phone in vibratory mode (no ring tone) in the cage. After the experimental period, passive avoidance behaviour and hippocampal morphology were studied. Results. Passive avoidance behaviour was significantly affected in mobile phone RF-EMR-exposed rats demonstrated as shorter entrance latency to the dark compartment when compared to the control rats. Marked morphological changes were also observed in the CA3 region of the hippocampus of the mobile phone-exposed rats in comparison to the control rats. Conclusion. Mobile phone RF-EMR exposure significantly altered the passive avoidance behaviour and hippocampal morphology in rats.

[1]  P. Knapp,et al.  Exposure to cell phone radiation up-regulates apoptosis genes in primary cultures of neurons and astrocytes , 2007, Neuroscience Letters.

[2]  I. Meral,et al.  Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs , 2007, Brain Research.

[3]  Sargo Aalto,et al.  Mobile Phone Affects Cerebral Blood Flow in Humans , 2006, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[4]  L. Squire,et al.  The primate hippocampal formation: evidence for a time-limited role in memory storage. , 1990, Science.

[5]  Purnyn' Oe.  Some conceptual aspects in studies of the autonomic nervous system , 2008 .

[6]  M. Mailankot,et al.  Spatial Memory Perfomance of Wistar Rats Exposed to Mobile Phone , 2009, Clinics.

[7]  S. Kamışlı,et al.  Ginkgo biloba prevents mobile phone-induced oxidative stress in rat brain. , 2004, Clinica chimica acta; international journal of clinical chemistry.

[8]  D. Kosson,et al.  Reaction to punishment, reflectivity, and passive avoidance learning in extraverts. , 1987, Journal of personality and social psychology.

[9]  O. Burešová,et al.  Techniques and Basic Experiments for the Study of Brain and Behavior , 1976 .

[10]  H. Lai,et al.  Single- and double-strand DNA breaks in rat brain cells after acute exposure to radiofrequency electromagnetic radiation. , 1996, International journal of radiation biology.

[11]  A. Crane,et al.  Effects of N-hydroxyethyl-pyrrolidinium methiodide, a choline analogue, on passive avoidance behaviour in mice , 1975, Neuropharmacology.

[12]  Ömer Akyol,et al.  Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits , 2002, Cell biochemistry and function.

[13]  M. Hilgert,et al.  Glucose plus choline improve passive avoidance behaviour and increase hippocampal acetylcholine release in mice , 2001, Neuroscience.

[14]  Dusan Sokolovic,et al.  Melatonin reduces oxidative stress induced by chronic exposure of microwave radiation from mobile phones in rat brain. , 2008, Journal of radiation research.

[15]  Gal Richter-Levin,et al.  Amygdala-hippocampus dynamic interaction in relation to memory , 2000, Molecular Neurobiology.

[16]  R. de Seze,et al.  Effect of an acute 900MHz GSM exposure on glia in the rat brain: a time-dependent study. , 2007, Toxicology.

[17]  C. Yardin,et al.  Apoptosis is Induced by Radiofrequency Fields through the Caspase-Independent Mitochondrial Pathway in Cortical Neurons , 2008, Radiation research.

[18]  P. Laming,et al.  Do glia contribute to behaviour? A neuromodulatory review. , 1989, Comparative biochemistry and physiology. A, Comparative physiology.

[19]  Joseph W. Harding,et al.  Effects of discrete kainic acid-induced hippocampal lesions on spatial and contextual learning and memory in rats , 1996, Brain Research.

[20]  R. de Seze,et al.  Effect of a chronic GSM 900 MHz exposure on glia in the rat brain. , 2008, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[21]  Lars Malmgren,et al.  Nerve cell damage in mammalian brain after exposure to microwaves from GSM mobile phones. , 2003, Environmental health perspectives.

[22]  Peter C. Blumbergs,et al.  Effect of mobile telephony on blood‐brain barrier permeability in the fetal mouse brain , 2006, Pathology.

[23]  H. Kimelberg,et al.  Neuronal–glial interactions and behaviour , 2000, Neuroscience & Biobehavioral Reviews.

[24]  R. Bartus,et al.  Age-related changes in passive avoidance retention: modulation with dietary choline. , 1980, Science.

[25]  S. Rose,et al.  Unilateral hippocampal lesions prevent recall of a passive avoidance task in day-old chicks , 1992, Neuroscience Letters.

[26]  Val Gebski,et al.  Effect of long‐term mobile communication microwave exposure on vascular permeability in mouse brain , 2002, Pathology.