transfer from adult somatic cells

We thank the technical staff of RIKEN GSC, M. Horishima, H. Ishizaki, N. Ota andY. Seki for sequencing; T. Yada for ORF prediction; A. Toyoda for technical supporton the sequence library preparation; C. Kawagoe for computer system support; andT. D. Taylor for discussion. This work was supported by a grant from the Programfor Promotion of Basic Research Activities for Innovation Biosciences (ProBRAIN)of the Bio-oriented Technology Research Advancement Institution, and Grants-in-Aidfor Scientific Research from the Japanese Ministry of Education, Science, Sports andCulture.Correspondence and requests for materials should be addressed to Y.S.(e-mail: sakaki@ims.u-tokyo.ac.jp) or H.I. (e-mail: iskw@biol.s.u-tokyo.ac.jp). Thecomplete sequence and the annotated data are available on our website(http://buchnera.gsc.riken.go.jp/). The sequence has been deposited with DDBJ underaccession number AP000398, AP001070 and AP001071 for chromosome, the pTrpplasmid and the pLeu plasmid, respectively.

[1]  R. Petters,et al.  Culture of pig embryos. , 2020, Journal of reproduction and fertility. Supplement.

[2]  I. Wilmut,et al.  "Viable Offspring Derived from Fetal and Adult Mammalian Cells" (1997), by Ian Wilmut et al. , 2014 .

[3]  I. Wilmut,et al.  Sheep cloned by nuclear transfer from a cultured cell line , 1996, Nature.

[4]  I Wilmut,et al.  Cell cycle co-ordination in embryo cloning by nuclear transfer. , 1996, Reviews of reproduction.

[5]  B. N. Day,et al.  Maintenance of unilateral pregnancy in the pig with induced corpora lutea. , 1971, Journal of animal science.

[6]  A. Colman,et al.  Somatic cell nuclear transfer in mammals: progress and applications. , 1999, Cloning.

[7]  B. N. Day,et al.  Development of pig embryos reconstructed by microinjection of cultured fetal fibroblast cells into in vitro matured oocytes. , 1999, Animal reproduction science.

[8]  R. Prather,et al.  Nuclear transplantation in early pig embryos. , 1989, Biology of reproduction.

[9]  Maurizio Zuccotti,et al.  Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei , 1998, Nature.

[10]  M. S. Peters,et al.  Artificial activation of porcine oocytes matured in vitro , 1991, Molecular reproduction and development.

[11]  M. Destrempes,et al.  Production of goats by somatic cell nuclear transfer , 1999, Nature Biotechnology.

[12]  C. Polge,et al.  The effect of reducing the number of embryos during early stages of gestation on the maintenance of pregnancy in the pig. , 1966, Journal of reproduction and fertility.

[13]  H. R. Tervit,et al.  Production of cloned lambs from an established embryonic cell line: a comparison between in vivo- and in vitro-matured cytoplasts. , 1997, Biology of reproduction.

[14]  A. Schnieke,et al.  Production of gene-targeted sheep by nuclear transfer from cultured somatic cells , 2000, Nature.

[15]  T. Kono,et al.  Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[16]  H. R. Tervit,et al.  Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. , 1999, Biology of reproduction.

[17]  F. A. P. León,et al.  Cloned transgenic calves produced from nonquiescent fetal fibroblasts. , 1998, Science.

[18]  D. Solter,et al.  Nuclear transplantation in mouse embryos. , 1983, The Journal of experimental zoology.

[19]  D. Solter,et al.  Nuclear transplantation in the mouse embryo by microsurgery and cell fusion. , 1983, Science.

[20]  J. Platt,et al.  Xenotransplantation: recent progress and current perspectives. , 1996, Current opinion in immunology.

[21]  J. Fulka,et al.  Nuclear transplantation in pigs: M-phase karyoplast to M-phase cytoplast fusion. , 1996, Reproduction, nutrition, development.