17q12‐21 amplicon, a novel recurrent genetic change in intestinal type of gastric carcinoma: A comparative genomic hybridization study

We studied DNA copy number changes in gastric cancer (GC) using comparative genomic hybridization (CGH) analysis on 35 resected gastric carcinomas (22 of the intestinal type and 13 of the diffuse type). Eighty‐three percent of the cases showed DNA copy number changes. Gains were more common than losses (median of 3 and 1 in primary tumors of the intestinal and diffuse type, respectively). The most common gains were detected on 20q [46%; 12 intestinal type (55%) and four diffuse type (31%)], 8q [37%; 10 intestinal type (45%) and three diffuse type (23%)], and 17q12‐21 [29%; all but one intestinal type (41%)]. The most frequent losses were detected on 18q [26%; all intestinal type (41%)] and on 4q [23%; all intestinal type (32%)]. High‐level amplifications were observed in the intestinal type of tumors at 17q12‐21 (three tumors), 20q (three tumors), 2p (one tumor), and 18q (one tumor). In the diffuse type, high‐level amplification was detected once at 13q. Genes Chromosom. Cancer 20:38–43, 1997. © 1997 Wiley‐Liss, Inc.

[1]  B. Vogelstein,et al.  Nomenclature: Vertebrate Mediators of TGFβ Family Signals , 1996, Cell.

[2]  Irene L Andrulis,et al.  MADR2 Maps to 18q21 and Encodes a TGFβ–Regulated MAD–Related Protein That Is Functionally Mutated in Colorectal Carcinoma , 1996, Cell.

[3]  P. Meltzer,et al.  Independent amplification and frequent co-amplification of three nonsyntenic regions on the long arm of chromosome 20 in human breast cancer. , 1996, Cancer research.

[4]  P. Meltzer,et al.  Hybrid selection of transcribed sequences from microdissected DNA: isolation of genes within amplified region at 20q11-q13.2 in breast cancer. , 1996, Cancer research.

[5]  S. Knuutila,et al.  DNA copy number losses in chromosome 14: an early change in gastrointestinal stromal tumors. , 1996, Cancer research.

[6]  Scott E. Kern,et al.  DPC4, A Candidate Tumor Suppressor Gene at Human Chromosome 18q21.1 , 1996, Science.

[7]  H. Scherthan,et al.  Advances in Brief Comparative Genomic in Situ Hybridization of Colon Carcinomas with Replication Error ' , 2006 .

[8]  H. Yokozaki,et al.  Frequent Amplification of the Cyclin E Gene in Human Gastric Carcinomas , 1995, Japanese journal of cancer research : Gann.

[9]  E. Tahara Molecular biology of gastric cancer , 1995, World journal of surgery.

[10]  J Piper,et al.  Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors , 1994, Genes, chromosomes & cancer.

[11]  J Piper,et al.  Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Fukayama,et al.  Molecular genetics for clinical management of colorectal carcinoma. 17p, 18q, and 22q loss of heterozygosity and decreased DCC expression are correlated with the metastatic potential , 1994, Cancer.

[13]  S. Fushida,et al.  Amplification of the c-erbB-2 gene in gastric carcinoma: correlation with survival. , 1993, Oncology.

[14]  H. Kim,et al.  Expression of cellular oncogenes in human gastric carcinoma: c‐myc, c–erb B2′ and c‐Ha‐ras , 1993, Journal of surgical oncology.

[15]  H. Ito,et al.  Frequent amplification of the c-met gene in scirrhous type stomach cancer. , 1992, Biochemical and biophysical research communications.

[16]  S. Hirohashi,et al.  Frequent loss of heterozygosity at the DCC locus in gastric cancer. , 1992, Cancer research.

[17]  S. Rakic,et al.  Serum gastrin levels in patients with intestinal and diffuse type of gastric cancer. , 1991, British Journal of Cancer.

[18]  G. Friedman,et al.  Helicobacter pylori infection and the risk of gastric carcinoma. , 1991, The New England journal of medicine.

[19]  M. Blaser,et al.  Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. , 1991, The New England journal of medicine.

[20]  J. Rhim,et al.  Amplification, overexpression, and rearrangement of the erbB-2 protooncogene in primary human stomach carcinomas. , 1989, Cancer research.

[21]  S. Watson,et al.  The in vitro growth response of primary human colorectal and gastric cancer cells to gastrin , 1989, International journal of cancer.

[22]  J. Mecklin,et al.  Carcinoma of the stomach and its heredity in young patients. , 1988, Scandinavian journal of gastroenterology.

[23]  Shirley A. Miller,et al.  A simple salting out procedure for extracting DNA from human nucleated cells. , 1988, Nucleic acids research.

[24]  P. Laurén,et al.  THE TWO HISTOLOGICAL MAIN TYPES OF GASTRIC CARCINOMA: DIFFUSE AND SO-CALLED INTESTINAL-TYPE CARCINOMA. AN ATTEMPT AT A HISTO-CLINICAL CLASSIFICATION. , 1965, Acta pathologica et microbiologica Scandinavica.