Evaluation of a pretreatment process for improved methane production from grass silage.pdf

Hydrolysis is a limiting step an anaerobic digestion. Hydrolytic pre-treatment of grass silage was evaluated at thermophilic temperature (55 °C), and the effect of organic loading rates (OLR) and hydraulic retention time (HRT) was studied. Two lab scale stainless steel continuously stirred reactors (10 L) and 12 glass reactors (2 L) were used. OLRs of 6.5, 5, 2.5 Kg VS m -3 d -1 and 10, 6, 4, 2 days HRT were evaluated. The parameters assessed as indicators for the level of hydrolysis were volatile solids destruction (VSD), volatile fatty acids (VFA) produced and soluble chemical oxygen demand (sCOD). Biological methane potential tests at mesophilic temperature (38 °C) were carried out for the evaluation of the effect of hydrolysis on the methanogenesis step.

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