Antifungal mechanism of FK463 against Candida albicans and Aspergillus fumigatus.

Cell free 1,3-β-D-glucan synthesis by Candida albicans ATCC90028 (purchased from the American Type Culture Collection, VA, USA) and by Aspergillus fumigatus TIMM0063 (kindly gifted from the Teikyo University Institute of Medical Mycology, Tokyo, Japan) were studied by the methods described below. C. albicans was precultured in YPD broth-A [1% w/v of Bacto-peptone, 0.5% w/v yeast extract, 2% w/v glucose] at 35°C overnight. Preculture was inoculated into fresh same medium (inoculum=10% of total volume) and grown for 6 hours at 35°C with shaking. Cells were harvested by centrifugation