High throughput cloning with restriction enzymes.
暂无分享,去创建一个
[1] T. Moore,et al. Human ORFeome version 1.1: a platform for reverse proteomics. , 2004, Genome research.
[2] H. Nojima,et al. High efficiency transformation of Escherichia coli with plasmids. , 1990, Gene.
[3] K. Büssow,et al. An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography , 2003, BMC biotechnology.
[4] H. Lehrach,et al. A catalog of human cDNA expression clones and its application to structural genomics , 2004, Genome Biology.
[5] J. Sambrook,et al. Molecular Cloning: A Laboratory Manual , 2001 .
[6] D. Moras,et al. Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli. , 2005, Analytical biochemistry.
[7] J. Hartley,et al. DNA cloning using in vitro site-specific recombination. , 2000, Genome research.
[8] Konrad Büssow,et al. Vectors for co-expression of an unrestricted number of proteins , 2007, Nucleic acids research.
[9] Konrad Büssow,et al. Facilities and methods for the high-throughput crystal structural analysis of human proteins. , 2003, Accounts of chemical research.
[10] R. Evans,et al. Ligation independent cloning irrespective of restriction site compatibility. , 1997, Nucleic acids research.
[11] P. D. de Jong,et al. Ligation-independent cloning of PCR products (LIC-PCR). , 1990, Nucleic acids research.
[12] R. Owens,et al. Recombinant protein expression and solubility screening in Escherichia coli: a comparative study. , 2006, Acta crystallographica. Section D, Biological crystallography.
[13] H. Lehrach,et al. An efficient and economic enhancer mix for PCR. , 2006, Biochemical and biophysical research communications.
[14] D. Engelke,et al. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. , 1990, Analytical biochemistry.
[15] S. Voss,et al. Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification. , 1997, Protein engineering.
[16] H. Eickhoff,et al. Development of a technology for automation and miniaturization of protein crystallization. , 2001, Journal of biotechnology.
[17] Peer Bork,et al. Microbial Cell Factories Structural Genomics of Human Proteins – Target Selection and Generation of a Public Catalogue of Expression Clones , 2022 .