Proteomics studies of traumatic brain injury.

Publisher Summary This chapter discusses the proteomics studies of traumatic brain injury. Traumatic brain injury (TBI) or traumatic head injury is characterized as a direct physical impact or trauma to the head causing brain injury. There is mechanical compression-induced direct tissue injury often associated with hemorrhage and contusion at the site of impact. The models of TBI include controlled cortical impact (CCI) model, a fluid percussion model and vertical weight drop models. The sample types that can be exploited for the proteomics analysis include brain tissues, cerebrospinal fluid (CSF), and blood (serum and plasma). The protein separation methods used for proteomic analysis are: (1) two-dimensional gel isoelectrofocusing/electrophoresis and (2) multidimensional liquid chromatography (LC). Proteomics approaches to identify and develop clinically useful biomarkers for brain injury from trauma, disease, or drugs involving protein separation by gels or LC, coupled with mass spectrometry, provide a potent and novel methodological array in detection of biomarkers of CNS injury either alone or in combination. The TBI proteomics core technologies will provide an integrative approach to genomic and proteomics information by developing a common portal architecture; the TBI proteomics portal.

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