Budding and braking news about clathrin-mediated endocytosis.

Clathrin-mediated endocytosis (CME) is the predominate mechanism of endocytosis in eukaryotes, but an understanding of this mechanism in plants has lagged behind yeast and mammalian systems. The generation of Arabidopsis mutant libraries, and the development of the molecular tools and equipment necessary to characterize these plant lines has led to an astonishing number of new insights into the mechanisms of membrane trafficking in plants. Over the past few years progress has been made on identifying, and in some instances confirming, the core components of CME in plants. This review focuses on the recent progress made in the understanding of the mechanism and regulation of CME in plants.

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[2]  G. Jürgens,et al.  Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis , 2009, The EMBO journal.

[3]  Xu Chen,et al.  Clathrin-mediated endocytosis: the gateway into plant cells. , 2011, Current opinion in plant biology.

[4]  I. Hwang,et al.  Arabidopsis μ-adaptin subunit AP1M of adaptor protein complex 1 mediates late secretory and vacuolar traffic and is required for growth , 2013, Proceedings of the National Academy of Sciences.

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[7]  S. Bednarek,et al.  Cytoskeletal and membrane dynamics during higher plant cytokinesis. , 2013, The New phytologist.

[8]  Martina Beck,et al.  Salicylic acid interferes with clathrin-mediated endocytic protein trafficking , 2013, Proceedings of the National Academy of Sciences.

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[11]  Xiaorong Wan,et al.  Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid, Abscisic Acid and High Boron Supply , 2013, International journal of molecular sciences.

[12]  D. Inzé,et al.  Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins[W] , 2006, The Plant Cell Online.

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[14]  I. Hwang,et al.  Adaptor Protein Complex 2–Mediated Endocytosis Is Crucial for Male Reproductive Organ Development in Arabidopsis[W] , 2013, Plant Cell.

[15]  Sebastian Y Bednarek,et al.  Variable-angle epifluorescence microscopy: a new way to look at protein dynamics in the plant cell cortex. , 2008, The Plant journal : for cell and molecular biology.

[16]  K. Haglund,et al.  The role of ubiquitylation in receptor endocytosis and endosomal sorting , 2012, Journal of Cell Science.

[17]  Zhenbiao Yang,et al.  A ROP GTPase-Dependent Auxin Signaling Pathway Regulates the Subcellular Distribution of PIN2 in Arabidopsis Roots , 2012, Current Biology.

[18]  Ying Fu,et al.  Cell Surface- and Rho GTPase-Based Auxin Signaling Controls Cellular Interdigitation in Arabidopsis , 2010, Cell.

[19]  Zhenbiao Yang,et al.  ROP GTPase-Dependent Actin Microfilaments Promote PIN1 Polarization by Localized Inhibition of Clathrin-Dependent Endocytosis , 2012, PLoS biology.

[20]  Ying Fu,et al.  Arabidopsis Interdigitating Cell Growth Requires Two Antagonistic Pathways with Opposing Action on Cell Morphogenesis , 2005, Cell.

[21]  Vera Bandmann,et al.  Uptake of fluorescent nano beads into BY2‐cells involves clathrin‐dependent and clathrin‐independent endocytosis , 2012, FEBS letters.

[22]  Simon C Watkins,et al.  Epsin 1 is a Polyubiquitin‐Selective Clathrin‐Associated Sorting Protein , 2006, Traffic.

[23]  Benjamin D. Sellers,et al.  Conformation switching of clathrin light chain regulates clathrin lattice assembly. , 2010, Developmental cell.

[24]  Michael Sauer,et al.  AUXIN BINDING PROTEIN1: The Outsider , 2011, Plant Cell.

[25]  N. Sauer,et al.  Routes to the Tonoplast: The Sorting of Tonoplast Transporters in Arabidopsis Mesophyll Protoplasts[W] , 2012, Plant Cell.

[26]  S. Bednarek,et al.  The Arabidopsis Dynamin-Related Protein2 Family Is Essential for Gametophyte Development[C][W] , 2010, Plant Cell.

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[30]  F. Baluška,et al.  Endocytosis, Actin Cytoskeleton, and Signaling1 , 2004, Plant Physiology.

[31]  A. Avni,et al.  EHD1 Functions in Endosomal Recycling and Confers Salt Tolerance , 2013, PloS one.

[32]  J. Ybe,et al.  Light Chain C‐Terminal Region Reinforces the Stability of Clathrin Heavy Chain Trimers , 2007, Traffic.

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[34]  Takashi Kawase,et al.  Identification and Dynamics of Arabidopsis Adaptor Protein-2 Complex and Its Involvement in Floral Organ Development[W] , 2013, Plant Cell.

[35]  M. Horowitz,et al.  AtEHDs, novel Arabidopsis EH-domain-containing proteins involved in endocytosis. , 2008, The Plant journal : for cell and molecular biology.

[36]  R. Lew,et al.  Turgor Regulation in Osmotically Stressed Arabidopsis Epidermal Root Cells. Direct Support for the Role of Inorganic Ion Uptake as Revealed by Concurrent Flux and Cell Turgor Measurements1 , 2002, Plant Physiology.

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[38]  E. Eisenberg,et al.  Multiple Roles of Auxilin and Hsc70 in Clathrin‐Mediated Endocytosis , 2007, Traffic.

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[42]  Z. Mou,et al.  Salicylic acid and its function in plant immunity. , 2011, Journal of integrative plant biology.

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[44]  O. Leyser,et al.  Strigolactone Can Promote or Inhibit Shoot Branching by Triggering Rapid Depletion of the Auxin Efflux Protein PIN1 from the Plasma Membrane , 2013, PLoS biology.

[45]  M. Otegui,et al.  Plant endosomal trafficking pathways. , 2011, Current opinion in plant biology.

[46]  B. Scheres,et al.  Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 Function in Epidermal Cell Polarityw⃞ , 2005, The Plant Cell Online.

[47]  J. Friml,et al.  Clathrin Mediates Endocytosis and Polar Distribution of PIN Auxin Transporters in Arabidopsis[W] , 2011, Plant Cell.

[48]  M. Van Montagu,et al.  ADP-ribosylation factor machinery mediates endocytosis in plant cells , 2010, Proceedings of the National Academy of Sciences.

[49]  Haiyang Wang,et al.  Dynamic analysis of Arabidopsis AP2 σ subunit reveals a key role in clathrin-mediated endocytosis and plant development , 2013, Development.

[50]  A ROP GTPase Signaling Pathway Controls Cortical Microtubule Ordering and Cell Expansion in Arabidopsis , 2009, Current Biology.

[51]  D. MacLean,et al.  Spatio-Temporal Cellular Dynamics of the Arabidopsis Flagellin Receptor Reveal Activation Status-Dependent Endosomal Sorting[C][W] , 2012, Plant Cell.

[52]  Martin Bringmann,et al.  Patterning and Lifetime of Plasma Membrane-Localized Cellulose Synthase Is Dependent on Actin Organization in Arabidopsis Interphase Cells1[W] , 2013, Plant Physiology.

[53]  P. Pimpl,et al.  Ubiquitin initiates sorting of Golgi and plasma membrane proteins into the vacuolar degradation pathway , 2012, BMC Plant Biology.

[54]  M. Sauer,et al.  MTV1 and MTV4 Encode Plant-Specific ENTH and ARF GAP Proteins That Mediate Clathrin-Dependent Trafficking of Vacuolar Cargo from the Trans-Golgi Network[C][W] , 2013, Plant Cell.

[55]  D. Sahlender,et al.  The Fifth Adaptor Protein Complex , 2011, PLoS biology.

[56]  F. Brodsky,et al.  Diversity of clathrin function: new tricks for an old protein. , 2012, Annual review of cell and developmental biology.

[57]  I. Hwang,et al.  Phosphoinositides Regulate Clathrin-Dependent Endocytosis at the Tip of Pollen Tubes in Arabidopsis and Tobacco[W] , 2010, Plant Cell.

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[59]  I. Hwang,et al.  The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis[W] , 2013, Plant Cell.

[60]  D. Loerke,et al.  Global and local regulation of clathrin-coated pit dynamics detected on patterned substrates. , 2009, Biophysical journal.

[61]  D. Inzé,et al.  Adaptin-like protein TPLATE and clathrin recruitment during plant somatic cytokinesis occurs via two distinct pathways , 2010, Proceedings of the National Academy of Sciences.

[62]  Edda Klipp,et al.  Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation , 2010, European Biophysics Journal.

[63]  T. Kirchhausen,et al.  Eps15 Is a Component of Clathrin-coated Pits and Vesicles and Is Located at the Rim of Coated Pits* , 1996, The Journal of Biological Chemistry.

[64]  Nan Jiang,et al.  Clathrin Light Chains Regulate Clathrin-Mediated Trafficking, Auxin Signaling, and Development in Arabidopsis[C][W][OA] , 2013, Plant Cell.

[65]  W. J. Lucas,et al.  A Membrane Microdomain-Associated Protein, Arabidopsis Flot1, Is Involved in a Clathrin-Independent Endocytic Pathway and Is Required for Seedling Development[C][W] , 2012, Plant Cell.

[66]  K. Tamura,et al.  The AP-1 μ adaptin is required for KNOLLE localization at the cell plate to mediate cytokinesis in Arabidopsis. , 2013, Plant & cell physiology.

[67]  A. Nakano,et al.  DRP1A Is Responsible for Vascular Continuity Synergistically Working with VAN3 in Arabidopsis1 , 2005, Plant Physiology.

[68]  I. Hwang,et al.  The AP-3 adaptor complex is required for vacuolar function in Arabidopsis , 2011, Cell Research.

[69]  Comert Kural,et al.  Actin dynamics counteract membrane tension during clathrin-mediated endocytosis , 2011, Nature Cell Biology.

[70]  Crislyn D'Souza-Schorey,et al.  ARF proteins: roles in membrane traffic and beyond , 2006, Nature Reviews Molecular Cell Biology.

[71]  D. Boettner,et al.  Lessons from yeast for clathrin-mediated endocytosis , 2011, Nature Cell Biology.

[72]  Alan M. Jones,et al.  ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in Arabidopsis , 2010, Cell.

[73]  L. Meisel,et al.  Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes , 2008, Proceedings of the National Academy of Sciences.

[74]  Lei Lei,et al.  The Endocytosis of Cellulose Synthase in Arabidopsis Is Dependent on μ2, a Clathrin-Mediated Endocytosis Adaptin1[W][OPEN] , 2013, Plant Physiology.

[75]  C. Curie,et al.  Monoubiquitin-dependent endocytosis of the IRON-REGULATED TRANSPORTER 1 (IRT1) transporter controls iron uptake in plants , 2011, Proceedings of the National Academy of Sciences.

[76]  J. Yates,et al.  Regulation of ubiquitin-dependent cargo sorting by multiple endocytic adaptors at the plasma membrane , 2013, Proceedings of the National Academy of Sciences.