Association between Genotypes of Growth Hormone Gene and Semen Quality Traits in Cow Bulls and Buffalo Bulls

The aim of this study was to detect the genetic polymorphism within the growth hormone gene Alu 1 locus in both Egyptian river buffalo and Holstein bulls using PCR-RFLP technique. The study was conducted on a total of 64 bulls which included 34 Egyptian buffalo bulls and 30 Holstein bulls. All the investigated buffalo bulls were genotyped as LL genotype. In turn, 25 and 5 Holstein bulls were of LL and LV genotypes, respectively while none of the examined cow bulls was genotyped as VV genotype. The genotype frequencies of LL and LV genotypes in Holstein bulls were 83.3% and 16.7%, respectively while the allelic frequencies of L and V alleles were 0.92 and 0.08, respectively. The obtained results revealed a significant positive association (P<0.05) between the LV genotype and semen volume, percentage of individual motility and post-thawing motility in the examined cow bulls while no sign of association with other investigated semen quality traits was observed. In conclusion, the buffalo growth hormone gene was found to be monomorphic for this locus while polymorphism in growth hormone can be a candidate gene for selection of Holstein bulls used in artificial insemination centers where we can early predict their fertility after the birth.

[1]  O. Othman,et al.  Evaluation of DNA Polymorphism in Egyptian Buffalo Growth Hormone and Its Receptor Genes , 2012 .

[2]  J. Bidanel.  Biology and Genetics of reproduction , 2011 .

[3]  A. Javanmard,et al.  Association between GH encoding gene polymorphism and semen characteristics in Iranian Holstein bulls , 2011 .

[4]  Chun-yan Zhang,et al.  Effects of MboII and BspMI polymorphisms in the gonadotropin releasing hormone receptor (GnRHR) gene on sperm quality in Holstein bulls , 2011, Molecular Biology Reports.

[5]  Changfa Wang,et al.  Correlation analysis between three novel SNPs of the Src gene in bovine and milk production traits , 2010, Molecular Biology Reports.

[6]  Jiabao Zhang,et al.  Effects of novel single nucleotide polymorphisms of the FSH beta-subunit gene on semen quality and fertility in bulls. , 2009, Animal reproduction science.

[7]  Dheer Singh,et al.  CYP19 (cytochrome P450 aromatase) gene polymorphism in murrah buffalo heifers of different fertility performance. , 2009, Research in veterinary science.

[8]  H. Yardibi,et al.  Associations of growth hormone gene polymorphisms with milk production traits in South Anatolian and East Anatolian Red cattle. , 2009 .

[9]  M. Bonyadi,et al.  A MspI PCR-RFLP within bovin growth hormone gene and its association with sperm quality traits in Iranian Holstein bulls. , 2009 .

[10]  Hai-Guo Jin,et al.  Association of genetic polymorphism inGH gene with milk production traits in Beijing Holstein cows , 2005, Journal of Biosciences.

[11]  S. Galal.  FARM ANIMAL GENETIC RESOURCES IN EGYPT: FACTSHEET , 2007 .

[12]  L. Fésüs,et al.  Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population , 2006 .

[13]  M. Thomas,et al.  Evaluation of DNA polymorphisms involving growth hormone relative to growth and carcass characteristics in Brahman steers. , 2006, Genetics and molecular research : GMR.

[14]  K. Wimmers,et al.  Candidate gene markers for sperm quality and fertility of boar. , 2006, Animal reproduction science.

[15]  D. A. Palmieri,et al.  Growth and carcass traits associated with GH1/Alu I and POU1F1/Hinf I gene polymorphisms in Zebu and crossbred beef cattle , 2006 .

[16]  K. Wimmers,et al.  Polymorphisms in candidate genes as markers for sperm quality and boar fertility. , 2005, Animal genetics.

[17]  S. Harvey,et al.  Growth hormone: roles in male reproduction. , 2000, Endocrine.

[18]  M. Szydłowski,et al.  Growth hormone gene polymorphism and reproductive performance of AI bulls. , 1999, Theriogenology.

[19]  D. Portetelle,et al.  Candidate gene markers associated with somatotropic axis and milk selection. , 1999, Domestic animal endocrinology.

[20]  J. Dekkers,et al.  Environmental, management, and genetic factors affecting semen production in Holstein bulls. , 1998, Journal of dairy science.

[21]  B. Colenbrander,et al.  Optimizing semen production for artificial insemination in swine. , 2020, Journal of reproduction and fertility. Supplement.

[22]  R. Oko,et al.  Abnormal Morphology of Bovine Spermatozoa , 1991 .

[23]  J. Donelson,et al.  Nucleotide sequence of the bovine growth hormone chromosomal gene , 1983, Molecular and Cellular Endocrinology.

[24]  H. Grüneberg,et al.  Introduction to quantitative genetics , 1960 .