Direct Determination of Saccharin and Acesulfame-K in Sweeteners and Fruit Juices Powders

A simple and rapid analytical procedure for determination of saccharin (SAC) and acesulfame-K (AC-K) based on UV-vis measurements and partial least squares (PLS) was proposed. Thus, an experimental design at levels 2 and 15 mg l−1 for SAC and 2 and 20 mg l−1 for AC-K was applied. Because real samples usually contain SAC and AC-K combined with aspartame (ASP), this interference was also included in the model. The procedure was successfully applied for SAC and AC-K simultaneous determination in sweeteners and fruit juice powders, without any separation step to remove ASP. The results were validated by using spiked samples, and the obtained recoveries were satisfactory.

[1]  Z. Ni,et al.  Separation and determination of four artificial sweeteners and citric acid by high-performance anion-exchange chromatography , 1997 .

[2]  M. Guardia,et al.  A clean method for flow injection spectrophotometric determination of cyclamate in table sweeteners , 2005 .

[3]  S. Garrigues,et al.  Sweeteners determination in table top formulations using FT-Raman spectrometry and chemometric analysis , 2004 .

[4]  J. Wang,et al.  Simultaneous determination of artificial sweeteners, preservatives, caffeine, theobromine and theophylline in food and pharmaceutical preparations by ion chromatography. , 2001, Journal of chromatography. A.

[5]  E. V. Thomas,et al.  Partial least-squares methods for spectral analyses. 1. Relation to other quantitative calibration methods and the extraction of qualitative information , 1988 .

[6]  Joint Fao.  Summary of evaluations performed by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) 1956-1993 (first through forty-first meetings) , 1994 .

[7]  Alejandro C. Olivieri,et al.  Wavelength selection by net analyte signals calculated with multivariate factor-based hybrid linear analysis (HLA). A theoretical and experimental comparison with partial least-squares (PLS) , 1999 .

[8]  Colin F. Poole,et al.  Encyclopedia of analytical science , 1995 .

[9]  R. Peña,et al.  Sequential injection analysis-based flow system for the enzymatic determination of aspartame , 2004 .

[10]  M. Boyce.  Simultaneous determination of antioxidants, preservatives and sweeteners permitted as additives in food by mixed micellar electrokinetic chromatography. , 1999, Journal of chromatography. A.

[11]  Alejandro C. Olivieri,et al.  MVC1: an integrated MatLab toolbox for first-order multivariate calibration , 2004 .

[12]  A. Olivieri,et al.  Determination of bromhexine in cough-cold syrups by absorption spectrophotometry and multivariate calibration using partial least-squares and hybrid linear analyses. Application of a novel method of wavelength selection. , 1999, Talanta.

[13]  L. Pezza,et al.  Potentiometric determination of saccharin in commercial artificial sweeteners using a silver electrode , 2003 .

[14]  A. Olivieri,et al.  Determination of the minor component bromhexine in cotrimoxazole-containing tablets by absorption spectrophotometry and partial least-squares (PLS-1) multivariate calibration. , 2000, Journal of pharmaceutical and biomedical analysis.

[15]  Yan Zhu,et al.  Separation and simultaneous determination of four artificial sweeteners in food and beverages by ion chromatography. , 2005, Journal of chromatography. A.

[16]  O. Fatibello‐Filho,et al.  Solid-phase reactor with copper(II) phosphate for flow-injection spectrophotometric determination of aspartame in tabletop sweeteners , 1999 .

[17]  K. Vytras,et al.  Direct simultaneous determination of eight sweeteners in foods by capillary isotachophoresis. , 2006, Journal of separation science.