Rapid changes in the expression of glial cell proteins caused by experimental retinal detachment.

We examined the expression of several proteins normally present in Müller's glia after the production of experimental retinal detachment in adult cats. Retinas were detached for one-half to seven days, after which the tissue was processed for correlative immunocytochemistry and biochemistry. Previous studies demonstrated that the intermediate filament proteins glial fibrillary acidic protein and vimentin, increase after long-term retinal detachment (30 to 60 days), whereas glutamine synthetase, carbonic anhydrase C, and cellular retinaldehyde-binding protein all decrease to barely detectable levels. Alterations in Müller cell protein expression are rapid and specific events that can be detected as early as two days after retinal detachment. By seven days, levels of protein expression are similar to those in the long-term retinal detachments. Within the first week after injury the Müller cell processes hypertrophy and begin forming glial scars, which indicates that early intervention may be required to halt or reverse the effects of detachment.

[1]  T. Aaberg,et al.  Glial cell proliferation in human retinal detachment with massive periretinal proliferation. , 1977, American journal of ophthalmology.

[2]  S. Feinstein,et al.  Glial fibrillary acidic protein and its mRNA: ultrastructural detection and determination of changes after CNS injury. , 1992, Journal of structural biology.

[3]  Don H. Anderson,et al.  Glial fibrillary acidic protein increases in Müller cells after retinal detachment. , 1987, Experimental eye research.

[4]  Experimental Retinal Detachment in the Owl Monkey , 1968 .

[5]  R. Goldman,et al.  Intermediate Filaments: Possible Functions as Cytoskeletal Connecting Links Between the Nucleus and the Cell Surface a , 1985, Annals of the New York Academy of Sciences.

[6]  T. Burton,et al.  Changing concepts of failures after retinal detachment surgery. , 1979, Archives of ophthalmology.

[7]  R. Machemer Pathogenesis and classification of massive periretinal proliferation. , 1978, The British journal of ophthalmology.

[8]  Foulds Ws EXPERIMENTAL RETINAL DETACHMENT. , 1963 .

[9]  Don H. Anderson,et al.  Changes in the expression of specific Müller cell proteins during long-term retinal detachment. , 1989, Experimental eye research.

[10]  Don H. Anderson,et al.  Intraretinal proliferation induced by retinal detachment. , 1991, Investigative ophthalmology & visual science.

[11]  David J. Wilson,et al.  Histopathologic study of the effect of retinal detachment surgery on 49 eyes obtained post mortem. , 1987, American journal of ophthalmology.

[12]  R. Liem,et al.  Suppression by antisense mRNA demonstrates a requirement for the glial fibrillary acidic protein in the formation of stable astrocytic processes in response to neurons , 1991, The Journal of cell biology.

[13]  D. Anderson,et al.  Morphological recovery in the reattached retina. , 1986, Investigative ophthalmology & visual science.

[14]  A. Moscona Chapter 4 On glutamine synthetase, carbonic anhydrase and Müller glia in the retina , 1983 .

[15]  M. Berry,et al.  Observations on the astrocyte response to a cerebral stab wound in adult rats , 1985, Brain Research.

[16]  B. Matsumoto,et al.  Preparation of Retinas for Studying Photoreceptors with Confocal Microscopy , 1993 .

[17]  D. Anderson,et al.  Changes in intermediate filament immunolabeling occur in response to retinal detachment and reattachment in primates. , 1990, Investigative ophthalmology & visual science.

[18]  I. Wallow,et al.  Müller's cell involvement in proliferative diabetic retinopathy. , 1987, Archives of ophthalmology.

[19]  A. M. Potts,et al.  An experimental study of retinal detachments. , 1966, American journal of ophthalmology.

[20]  R. Machemer,et al.  Glial cell proliferation in retinal detachment (massive periretinal proliferation). , 1975, American journal of ophthalmology.

[21]  D. Anderson,et al.  Retinal detachment in the cat: the outer nuclear and outer plexiform layers. , 1983, Investigative ophthalmology & visual science.

[22]  D. Anderson,et al.  Recovery of photoreceptor outer segment length and analysis of membrane assembly rates in regenerating primate photoreceptor outer segments. , 1993, Investigative Ophthalmology and Visual Science.

[23]  D. Anderson,et al.  Retinal detachment in the cat: the pigment epithelial-photoreceptor interface. , 1983, Investigative ophthalmology & visual science.

[24]  M. Lavail,et al.  GLUTAMINE SYNTHETASE IN THE NORMAL AND DYSTROPHIC MOUSE RETINA , 1971, The Journal of cell biology.

[25]  J. Boya,et al.  Co-expression of glial fibrillary acidic protein and vimentin in reactive astrocytes following brain injury in rats. , 1991, Brain research.

[26]  A. Kroll,et al.  Experimental retinal detachment in the owl monkey. 3. Electron microscopy of retina and pigment epithelium. , 1968, American journal of ophthalmology.