Photocatalytic Inactivation of Bioaerosols by TiO2 Coated Membrane

Indoor air pollution by microbial contaminants is increasingly receiving attention as a public health problem. Under a suitable environment, such as in heating, ventilation and air conditioning (HVAC) system, airborne bacteria are able to proliferate and grow causing various allergies and illnesses. This can be particularly serious in tropical regions due to high relative humidity and warm temperatures all round the year. Application of photocatalysis using UV-A and TiO2 to inactivate air-borne bacteria is relatively new and systematic parametric study is required for the engineering design of a process based on this technology. This study investigates the effects of TiO2 mediated inactivation of various bacterial species in batch and continuous systems using different TiO2 loadings and radiation intensities. Gram-negative bacteria, E. coli and two Gram-positive bacteria, Microbacterium sp. and Bacillus subtilis were used for the inactivation studies. In both systems, inactivation rates of Gram-negative E. coli are higher than the Gram-positive Bacillus subtilis and Microbacterium sp. and the inactivation rates increased in presence of TiO2 for all bacteria. Depending on the type of bacteria, TiO2 loading and light intensity, an increase of 1.3-5.8 times in the inactivation rates was obtained from those in the absence of TiO2. The inactivation rates in the batch and continuous systems were reasonably comparable. Inactivation rates in the continuous system are somewhat higher than those in the batch system due to the unaccounted loss of bacteria via adsorption and settling on the reactor walls in the flow system. The study demonstrates an approach that can be used for the designing of large scale systems for the treatment of bioaerosol.

[1]  Edward J. Wolfrum,et al.  Bactericidal Activity of Photocatalytic TiO2 Reaction: toward an Understanding of Its Killing Mechanism , 1999, Applied and Environmental Microbiology.

[2]  M Bekbölet,et al.  Inactivation of Escherichia coli by photocatalytic oxidation. , 1996, Chemosphere.

[3]  Lothar Erdinger,et al.  Disinfection of surfaces by photocatalytic oxidation with titanium dioxide and UVA light. , 2003, Chemosphere.

[4]  Hiroyuki Katayama,et al.  Photoreactivation of Escherichia coli after Low- or Medium-Pressure UV Disinfection Determined by an Endonuclease Sensitive Site Assay , 2002, Applied and Environmental Microbiology.

[5]  Zhongdang Xiao,et al.  Photochemical disinfection of Escherichia coli with a TiO2 colloid solution and a self-assembled TiO2 thin film , 1998 .

[6]  D. Blake,et al.  Photocatalytic oxidation of bacteria, bacterial and fungal spores, and model biofilm components to carbon dioxide on titanium dioxide-coated surfaces. , 2002, Environmental science & technology.

[7]  Tom D. Reynolds,et al.  Unit Operations and Processes in Environmental Engineering , 1995 .

[8]  D. Goswami A Review of Engineering Developments of Aqueous Phase Solar Photocatalytic Detoxification and Disinfection Processes , 1997 .

[9]  Hsuan-Liang Liu,et al.  Photocatalytic inactivation of Escherichia coli and Lactobacillus helveticus by ZnO and TiO2 activated with ultraviolet light , 2003 .

[10]  Edward J. Wolfrum,et al.  Application of the Photocatalytic Chemistry of Titanium Dioxide to Disinfection and the Killing of Cancer Cells , 1999 .

[11]  Xudong Yang,et al.  Photocatalytic oxidation for indoor air purification: a literature review , 2003 .

[12]  Edward J. Wolfrum,et al.  Mineralization of Bacterial Cell Mass on a Photocatalytic Surface in Air , 1998 .

[13]  Michael Brauer,et al.  Evaluation and Determinants of Airborne Bacterial Concentrations in School Classrooms , 2004, Journal of occupational and environmental hygiene.

[14]  M. Litter,et al.  Photocatalytic bactericidal effect of TiO2 on Enterobacter cloacae: Comparative study with other Gram (−) bacteria , 2003 .

[15]  P. V. Scarpino,et al.  The use of ultraviolet germicidal irradiation (UVGI) in disinfection of airborne bacteria , 2001 .

[16]  T. Nakajima,et al.  Photoelectrochemical sterilization of microbial cells by semiconductor powders , 1985 .

[17]  Cesar Pulgarin,et al.  Photocatalytical inactivation of E. coli: effect of (continuous-intermittent) light intensity and of (suspended-fixed) TiO2 concentration , 2003 .

[18]  Kayano Sunada,et al.  Studies on photokilling of bacteria on TiO2 thin film , 2003 .

[19]  P. A. Christensena,et al.  Photoelectrocatalytic and photocatalytic disinfection of E . coli suspensions by titanium dioxide , 2003 .

[20]  William P. Bahnfleth,et al.  Airborne respiratory diseases and mechanical systems for control of microbes , 1998 .

[21]  Madhumita B. Ray,et al.  Evaluation of three different lamp emission models using novel application of potassium ferrioxalate actinometry , 2004 .

[22]  Patricia Cruz,et al.  Detection and enumeration of airborne biocontaminants. , 2004, Current opinion in biotechnology.