Some practical guidelines for UV imaging in the protein crystallization laboratory

The use of UV imaging as a means of locating protein crystals is a fairly new tool, however not suitable for all protein crystallization trials. Practical examples of the strengths and some of the pitfalls of the technology are presented.

[1]  B. Matthews.  Solvent content of protein crystals. , 1968, Journal of molecular biology.

[2]  M. Eftink,et al.  Fluorescence quenching studies with proteins. , 1981, Analytical biochemistry.

[3]  F. Schmid,et al.  Biological Macromolecules: UV‐visible Spectrophotometry , 2001 .

[4]  Serge Massar,et al.  Optimality of the genetic code with respect to protein stability and amino-acid frequencies , 2001, Genome Biology.

[5]  Y. Engelborghs,et al.  The quenching of tryptophan fluorescence by protonated and unprotonated imidazole , 2005, European Biophysics Journal.

[6]  D. Platikanov,et al.  Proteins at fluid interfaces: adsorption layers and thin liquid films. , 2006, Advances in colloid and interface science.

[7]  T. Hollis.  Crystallization of protein-DNA complexes. , 2007, Methods in molecular biology.

[8]  Igor Jurisica,et al.  Protein crystallization analysis on the World Community Grid , 2009, Journal of Structural and Functional Genomics.

[9]  H. Gill.  Evaluating the efficacy of tryptophan fluorescence and absorbance as a selection tool for identifying protein crystals , 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

[10]  Christian Betzel,et al.  Efficient UV detection of protein crystals enabled by fluorescence excitation at wavelengths longer than 300 nm. , 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

[11]  M. Pusey.  Developing a Fluorescence-based Approach to Screening for Macromolecule Crystallization Conditions. , 2011, Crystal growth & design.

[12]  G. Simpson,et al.  Second-order nonlinear optical imaging of chiral crystals. , 2011, Annual review of analytical chemistry.

[13]  Roger A. Sayle,et al.  On the need for an international effort to capture, share and use crystallization screening data , 2012, Acta crystallographica. Section F, Structural biology and crystallization communications.

[14]  A. Dunker,et al.  Intrinsically Disordered Protein Analysis , 2012, Methods in Molecular Biology.