Analysis of cathepsin-K activity at tooth and dental implant sites and the potential of this enzyme in reflecting alveolar bone loss.

BACKGROUND Cathepsin-K is an enzyme involved in bone metabolism which may make this feature important for both natural teeth and dental implants. The aims of the present study are to comparatively analyze the gingival crevicular fluid (GCF)/peri-implant sulcus fluid (PISF) cathepsin-K levels of natural teeth and dental implants, and to assess the potential relationship between this biochemical parameter and alveolar bone loss around natural teeth and dental implants. METHODS Probing depth, bleeding on probing, gingival index, and plaque index clinical parameters were assessed, and GCF/PISF samples were obtained from natural teeth/dental implants presenting with either clinical health, gingivitis/peri-implant mucositis, or chronic periodontitis/peri-implantitis. Cathepsin-K activity levels of 42 GCF samples and 54 PISF samples were determined, and marginal bone loss (MBL) measures were calculated from digitalized standardized intraoral periapical radiographs obtained from natural teeth and dental implants by using cemento-enamel junction and the actual distance between two consecutive threads of the dental implant as reference points for natural teeth and dental implants, respectively. RESULTS Comparing the natural teeth group with dental implant group with regard to MBL measure, cathepsin-K activity, and GCF/PISF volume revealed no significant differences. In both natural teeth and dental implant groups, despite higher MBL measures, cathepsin-K activity, and GCF/PISF volumes with the presence of inflammation, it was the presence of alveolar bone loss that lead to significantly higher values for these parameters. CONCLUSION We suggest cathepsin-K as a biochemical parameter for monitoring periodontal/peri-implant alveolar bone loss.

[1]  E. Karabulut,et al.  Analysis of cathepsin-K levels in biologic fluids from healthy or diseased natural teeth and dental implants. , 2011, The International journal of oral & maxillofacial implants.

[2]  Anuj Sharma,et al.  Gingival crevicular fluid and serum vascular endothelial growth factor: their relationship in periodontal health, disease and after treatment. , 2011, Cytokine.

[3]  G. Garg,et al.  Effect of nonsurgical periodontal therapy on crevicular fluid levels of Cathepsin K in periodontitis. , 2009, Archives of oral biology.

[4]  T. Tözüm,et al.  A 12-Month Evaluation of Nitrite Oxide Metabolism Around Immediate and Conventionally Loaded Dental Implants , 2009, Implant dentistry.

[5]  T. Tözüm,et al.  Relationships between implant stability, image-based measures and nitric oxide levels. , 2008, Journal of oral rehabilitation.

[6]  E. Karabulut,et al.  Myeloperoxidase as a measure of polymorphonuclear leukocyte response in inflammatory status around immediately and delayed loaded dental implants: a randomized controlled clinical trial. , 2008, Clinical implant dentistry and related research.

[7]  I. Turkyilmaz,et al.  The impact of prosthetic design on the stability, marginal bone loss, peri-implant sulcus fluid volume, and nitric oxide metabolism of conventionally loaded endosseous dental implants: a 12-month clinical study. , 2008, Journal of periodontology.

[8]  E. Karabulut,et al.  Analysis of the inflammatory process around endosseous dental implants and natural teeth: myeloperoxidase level and nitric oxide metabolism. , 2007, The International journal of oral & maxillofacial implants.

[9]  M. Mogi,et al.  Expression of cathepsin-K in gingival crevicular fluid of patients with periodontitis. , 2007, Archives of oral biology.

[10]  H. Hatipoğlu,et al.  Impact of the distinct sampling area on volumetric features of gingival crevicular fluid. , 2007, Journal of periodontology.

[11]  T. Salo,et al.  Monitoring periodontal disease status in smokers and nonsmokers using a gingival crevicular fluid matrix metalloproteinase-8-specific chair-side test. , 2006, Journal of periodontal research.

[12]  T. Köse,et al.  Gingival crevicular fluid matrix metalloproteinase-13 levels and molecular forms in various types of periodontal diseases. , 2006, Oral diseases.

[13]  K. Akca,et al.  Implant-tooth-supported fixed partial prostheses: correlations between in vivo occlusal bite forces and marginal bone reactions. , 2006, Clinical oral implants research.

[14]  G. Watzek,et al.  Cathepsin K levels in the crevicular fluid of dental implants: a pilot study. , 2006, Journal of clinical periodontology.

[15]  R. Hanemaaijer,et al.  Gingival Tissue and Crevicular Fluid Co-operation in Adult Periodontitis , 2006, Journal of dental research.

[16]  E. Karabulut,et al.  Analysis of thte possible impact of inflammation severity and early and delayed loading on nitric oxide metabolism around dental implants. , 2005, The International journal of oral & maxillofacial implants.

[17]  Liran Levin,et al.  Marginal bone loss pattern around hydroxyapatite-coated versus commercially pure titanium implants after up to 12 years of follow-up. , 2005, The International journal of oral & maxillofacial implants.

[18]  T. Tözüm,et al.  Critical steps in electronic volume quantification of gingival crevicular fluid: the potential impact of evaporation, fluid retention, local conditions and repeated measurements. , 2004, Journal of periodontal research.

[19]  K. Fischer,et al.  Correlation of peri-implant health and myeloperoxidase levels: a cross-sectional clinical study. , 2004, Clinical oral implants research.

[20]  R. Haas,et al.  Radiologic follow-up of peri-implant bone loss around machine-surfaced and rough-surfaced interforaminal implants in the mandible functionally loaded for 3 to 7 years. , 2004, The International journal of oral & maxillofacial implants.

[21]  Yasuhiro Kobayashi,et al.  The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin k. , 2003, Journal of pharmacological sciences.

[22]  J. Bernard,et al.  Elastase, alpha2-macroglobulin and alkaline phosphatase in crevicular fluid from implants with and without periimplantitis. , 2002, Clinical oral implants research.

[23]  M. Soell,et al.  Cathepsin C, matrix metalloproteinases, and their tissue inhibitors in gingiva and gingival crevicular fluid from periodontitis-affected patients. , 2002, Journal of dental research.

[24]  S. Hellem,et al.  Nonsubmerged implants in the treatment of the edentulous lower jaw: a 5-year prospective longitudinal study of ITI hollow screws. , 2001, Clinical implant dentistry and related research.

[25]  G. Carlsson,et al.  Long-term marginal periimplant bone loss in edentulous patients. , 2000, The International journal of prosthodontics.

[26]  D. Brömme,et al.  Collagenolytic activity of cathepsin K is specifically modulated by cartilage-resident chondroitin sulfates. , 2000, Biochemistry.

[27]  K. Moriyama,et al.  Expression of cathepsin K mRNA during experimental tooth movement in rat as revealed by in situ hybridization. , 2000, Archives of oral biology.

[28]  T. Kocher,et al.  Longitudinal evaluation of aspartate aminotransferase in the crevicular fluid of implants with bone loss and signs of progressive disease. , 1999, The International journal of oral & maxillofacial implants.

[29]  Timo Sorsa,et al.  Inhibition of the Activities of Matrix Metalloproteinases 2, 8, and 9 by Chlorhexidine , 1999, Clinical Diagnostic Laboratory Immunology.

[30]  C. Overall,et al.  Activation of Neutrophil Collagenase in Periodontitis , 1999, Infection and Immunity.

[31]  K. Arvidson,et al.  Five-year prospective follow-up report of the Astra Tech Dental Implant System in the treatment of edentulous mandibles. , 1998, Clinical oral implants research.

[32]  L. Niemi,et al.  A 5-year prospective clinical study of Astra Tech dental implants supporting fixed bridges or overdentures in the edentulous mandible. , 1997, Clinical oral implants research.

[33]  S. Jepsen,et al.  Progressive peri-implantitis. Incidence and prediction of peri-implant attachment loss. , 1996, Clinical oral implants research.

[34]  C. Debouck,et al.  Cathepsin K, but Not Cathepsins B, L, or S, Is Abundantly Expressed in Human Osteoclasts (*) , 1996, The Journal of Biological Chemistry.

[35]  D. Aeppli,et al.  Crevicular fluid enzymes from endosseous dental implants and natural teeth. , 1996, The International journal of oral & maxillofacial implants.

[36]  R. Meffert.  Periodontitis vs. peri-implantitis: the same disease? The same treatment? , 1996, Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists.

[37]  N. Lang,et al.  Clinical parameters for the evaluation of dental implants. , 1994, Periodontology 2000.

[38]  C. Overall,et al.  Microbiota and crevicular fluid collagenase activity in the osseointegrated dental implant sulcus: a comparison of sites in edentulous and partially edentulous patients. , 1989, Journal of periodontal research.

[39]  R. Genco,et al.  Serum and Gingival Fluid Antibodies as Adjuncts in the Diagnosis of Actinobacillus actinomycetemcomitans Associated Periodontal Disease. , 1985, Journal of periodontology.

[40]  J. Hattingh,et al.  The quantification of individual proteins in crevicular gingival fluid. , 1985, Journal of periodontal research.

[41]  J. J. Pollock,et al.  Direct measurement of hepatitis B viral antibody and antigen markers in gingival crevicular fluid. , 1984, Oral surgery, oral medicine, and oral pathology.

[42]  Sano Kiyoko,et al.  An ultramicro-assay for proteins in biological fluids other than blood using a combination of agarose gel isoelectric focusing and silver staining. , 1984 .

[43]  J. Ainamo,et al.  Problems and proposals for recording gingivitis and plaque. , 1975, International dental journal.

[44]  H. Löe,et al.  PERIODONTAL DISEASE IN PREGNANCY. II. CORRELATION BETWEEN ORAL HYGIENE AND PERIODONTAL CONDTION. , 1964, Acta odontologica Scandinavica.

[45]  H. Löe,et al.  PERIODONTAL DISEASE IN PREGNANCY. I. PREVALENCE AND SEVERITY. , 1963, Acta odontologica Scandinavica.