An efficient somatic embryogenesis based plant regeneration from the hypocotyl of Catharanthus roseus

An efficient and rapid somatic embryogenesis based plant regeneration from hypocotyls of three Catharanthus roseus cultivars, Pacifica cherry red (PCR), Heatwave mix color (HWMC), and Mediterranean Rose Red (MRR) was standardized. Hypocotyl-derived primary calluses (HPC) were formed on callus induction medium (MSCP1) after 10 days of culture. Embryogenic calluses (EC) with somatic embryos were induced from HPC on proliferation medium (MSCP2) within 2 weeks of culture. Later, EC were selected and maintained on plantlet formation medium (MSCP3) with periodic subculturing at an interval of 2 weeks. Somatic embryos were germinated well into plantlets on MSCP3 within 6 weeks of culture. As sources of nitrogen, casein hydrolysate (CH; acid free) and L-proline were necessary as standard addenda in the plant regeneration protocol of C. roseus . The promotive effects of CH and L-proline not only decreased the culturing time but also increased the number of somatic embryos and their derived plantlets. The percentage of EC and regenerated plant initiated from hypocotyls were higher than those previously reported.

[1]  P. Choi,et al.  Assessing competence for adventitious shoot formation in hypocotyl explant cultures fromCatharanthus roseus cultivars , 2003, Journal of Plant Biology.

[2]  J. Snape,et al.  High-throughput Agrobacterium-mediated barley transformation , 2008, Plant Methods.

[3]  D. Kim,et al.  Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus , 2008, In Vitro Cellular & Developmental Biology - Plant.

[4]  J. Šamaj,et al.  Somatic embryogenesis and plant regeneration in Catharanthus roseus , 2007, Biologia Plantarum.

[5]  Wei Tang,et al.  Growth regulators affect primary and secondary somatic embryogenesis in Madagaskar periwinkle (Catharanthus roseus (L.) G. Don) at morphological and biochemical levels , 2007, Plant Growth Regulation.

[6]  D. L. DeBoer,et al.  Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.) , 1993, In Vitro Cellular & Developmental Biology - Plant.

[7]  S. Riazuddin,et al.  Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.) , 2006, Journal of Zhejiang University SCIENCE B.

[8]  K. San,et al.  Terpenoid indole alkaloid production by Catharanthus roseus hairy roots induced by Agrobacterium tumefaciens harboring rol ABC genes. , 2006, Biotechnology and bioengineering.

[9]  R. Verpoorte,et al.  The Catharanthus alkaloids: pharmacognosy and biotechnology. , 2004, Current medicinal chemistry.

[10]  Jang Ryol Liu,et al.  Plant Regeneration from Immature Zygotic Embryo-Derived Embryogenic Calluses and Cell Suspension Cultures of Catharanthus roseus , 2004, Plant Cell, Tissue and Organ Culture.

[11]  S. Y. Lee,et al.  Comparison of adventitious shoot formation in petiole explant cultures of 20 cultivars of Catharanthus roseus , 2003 .

[12]  J. Latimer,et al.  Growth Regulators , 2002 .

[13]  K. Rajasekaran,et al.  Induction of Highly Embryogenic Calli and Plant Regeneration in Upland (Gossypium hirsutum L.) and Pima (Gossypium barbadense L.) Cottons , 2001 .

[14]  S. Ogita,et al.  Efficient Plant Regeneration of Larix Kaempferi , 2001 .

[15]  L. van der Fits,et al.  ORCA3, a jasmonate-responsive transcriptional regulator of plant primary and secondary metabolism. , 2000, Science.

[16]  J. Shanks,et al.  Production of indole alkaloids by selected hairy root lines of Catharanthus roseus , 1993, Biotechnology and bioengineering.

[17]  J. Kutney,et al.  Alkaloid Production in Catharanthus roseus Cell Cultures VIII1 , 1981, Planta medica.

[18]  R. Phillips,et al.  Tissue Cultures of Maize (Zea mays L.): Initiation, Maintenance, and Organic Growth Factors 1 , 1974 .

[19]  W. Halperin,et al.  Ammonium Requirement for Embryogenesis in vitro , 1965, Nature.

[20]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[21]  F. Skoog,et al.  A revised medium for the growth and bioassay with tobacco tissue culture , 1962 .

[22]  M. Sanders,et al.  Influence of Amino Acids on Growth of Datura Embryos in Culture. , 1948, Proceedings of the National Academy of Sciences of the United States of America.