Partitioning and diffusion of large molecules in fibrous structures.

This paper consists of three parts. In the first part we present diffusion and partition coefficients of proteins in agarose that were measured with gel permeation chromatography. In the second part we present a partition model which includes the effects of solute concentration and of cosolutes. In the third part we modify and extend Ogston's diffusion equation to account for the effects of solute and fiber flexibility, solute concentration and cosolutes. We find good agreement between the proposed models and data from the literature.

[1]  B. Amsden.  Solute diffusion in hydrogels. , 1998 .

[2]  John F. Brady,et al.  Hindered transport of spherical macromolecules in fibrous membranes and gels , 1989 .

[3]  James T. Hsu,et al.  Experimental studies of restricted protein diffusion in an agarose matrix , 1992 .

[4]  R K Jain,et al.  Hindered diffusion in agarose gels: test of effective medium model. , 1996, Biophysical journal.

[5]  R K Jain,et al.  Diffusion and partitioning of proteins in charged agarose gels. , 1995, Biophysical journal.

[6]  J. Williams,et al.  Partition and permeation of dextran in polyacrylamide gel. , 1998, Biophysical journal.

[7]  L. Johansson,et al.  Diffusion and interaction in gels and solutions. 3. Theoretical results on the obstruction effect , 1991 .

[8]  R. C. Weast.  CRC Handbook of Chemistry and Physics , 1973 .

[9]  P. Wahl,et al.  Diffusion of proteins in Sepharose Cl-B gels. , 1992, Journal of chromatography.

[10]  T. Laurent,et al.  A theory of gel filtration and its exeperimental verification , 1964 .

[11]  P. Wahl,et al.  Study of the translational diffusion of macromolecules in beads of gel chromatography by the FRAP method. , 1988, Biophysical chemistry.

[12]  D. F. James,et al.  The permeability of fibrous porous media , 1986 .

[13]  J. Anderson,et al.  Partitioning and diffusion of proteins and linear polymers in polyacrylamide gels. , 1996, Biophysical journal.

[14]  H. A. Sober,et al.  Handbook of Biochemistry: Selected Data for Molecular Biology , 1971 .

[15]  E. Glandt,et al.  Partitioning of spherical particles into fibrous matrices: 2. Monte Carlo simulation , 1990 .

[16]  A. G. Ogston,et al.  The spaces in a uniform random suspension of fibres , 1958 .

[17]  G. K. Ackers,et al.  Restricted diffusion of macromolecules through agar-gel membranes. , 1962, Biochimica et biophysica acta.

[18]  P. Wahl,et al.  Diffusion of proteins in the chromatographic gel AcA-34 , 1991 .

[19]  P. Vonk.  Diffusion of large molecules in porous structures , 1994 .