Enhanced model‐based design of a high‐throughput three dimensional micromixer driven by alternating‐current electrothermal flow

We propose a 3D microfluidic mixer based on the alternating current electrothermal (ACET) flow. The ACET vortex is produced by 3D electrodes embedded in the sidewall of the microchannel and is used to stir the fluidic sample throughout the entire channel depth. An optimized geometrical structure of the proposed 3D micromixer device is obtained based on the enhanced theoretical model of ACET flow and natural convection. We quantitatively analyze the flow field driven by the ACET, and a pattern of electrothermal microvortex is visualized by the micro‐particle imaging velocimetry. Then, the mixing experiment is conducted using dye solutions with varying solution conductivities. Mixing efficiency can exceed 90% for electrolytes with 0.2 S/m (1 S/m) when the flow rate is 0.364 μL/min (0.728 μL/min) and the imposed peak‐to‐peak voltage is 52.5 V (35 V). A critical analysis of our micromixer in comparison with different mixer designs using a comparative mixing index is also performed. The ACET micromixer embedded with sidewall 3D electrodes can achieve a highly effective mixing performance and can generate high throughput in the continuous‐flow condition.

[1]  D A. Dunn,et al.  Challenges and solutions to ultra-high-throughput screening assay miniaturization: submicroliter fluid handling. , 2000, Drug discovery today.

[2]  Takehiko Kitamori,et al.  Fluid mixing using AC electrothermal flow on meandering electrodes in a microchannel , 2012, Electrophoresis.

[3]  Zhengdong Wang,et al.  Design and evaluation of an easily fabricated micromixer with three-dimensional periodic perturbation , 2011 .

[4]  I. Mezić,et al.  A multiscale measure for mixing , 2005 .

[5]  Chun Yang,et al.  Enhancement of electrokinetically driven microfluidic T‐mixer using frequency modulated electric field and channel geometry effects , 2009, Electrophoresis.

[6]  Robin H. Liu,et al.  Hybridization enhancement using cavitation microstreaming. , 2003, Analytical chemistry.

[7]  Thomas Laurell,et al.  Ultrasonic agitation in microchannels , 2004, Analytical and bioanalytical chemistry.

[8]  I. Mezić,et al.  Chaotic Mixer for Microchannels , 2002, Science.

[9]  Kwang-Yong Kim,et al.  Passive split and recombination micromixer with convergent–divergent walls , 2012 .

[10]  Brian N. Johnson,et al.  An integrated nanoliter DNA analysis device. , 1998, Science.

[11]  Jin-Woo Choi,et al.  A novel in-plane passive microfluidic mixer with modified Tesla structures. , 2004, Lab on a chip.

[12]  Hongyuan Jiang,et al.  Effect of the crossing-structure sequence on mixing performance within three-dimensional micromixers. , 2014, Biomicrofluidics.

[13]  T. Johnson,et al.  Rapid microfluidic mixing. , 2002, Analytical chemistry.

[14]  Rodney J. Y. Ho,et al.  ADVANCED DRUG DELIVERY , 2004 .

[15]  Enhanced electrothermal pumping with thin film resistive heaters , 2013, Electrophoresis.

[16]  Hongyuan Jiang,et al.  An effective splitting-and-recombination micromixer with self-rotated contact surface for wide Reynolds number range applications. , 2013, Biomicrofluidics.

[17]  S. Bhattacharjee,et al.  Study on the use of dielectrophoresis and electrothermal forces to produce on-chip micromixers and microconcentrators. , 2012, Biomicrofluidics.

[18]  Nurul Amziah Md Yunus,et al.  Continuous separation of colloidal particles using dielectrophoresis , 2013, Electrophoresis.

[19]  Josef Hormes,et al.  Microfluidic synthesis of nanomaterials. , 2008, Small.

[20]  Q. Yuan,et al.  Optimization of planar interdigitated microelectrode array for biofluid transport by AC electrothermal effect , 2014 .

[21]  Mandy L Y Sin,et al.  Electrothermal Fluid Manipulation of High-Conductivity Samples for Laboratory Automation Applications , 2010, JALA.

[22]  Ruey-Jen Yang,et al.  Electrokinetic mixing in microfluidic systems , 2007 .

[23]  Chun Yang,et al.  Continuous sorting and separation of microparticles by size using AC dielectrophoresis in a PDMS microfluidic device with 3‐D conducting PDMS composite electrodes , 2010, Electrophoresis.

[24]  Kristen L. Helton,et al.  Microfluidic Overview of Global Health Issues Microfluidic Diagnostic Technologies for Global Public Health , 2006 .

[25]  S. Krishnamoorthy,et al.  Numerical analysis of mixing by electrothermal induced flow in microfluidic systems. , 2007, Biomicrofluidics.

[26]  Chun Yang,et al.  DC-biased AC-electroosmotic and AC-electrothermal flow mixing in microchannels. , 2009, Lab on a chip.

[27]  Krishnaswamy Nandakumar,et al.  Optimal patterning of heterogeneous surface charge for improved electrokinetic micromixing , 2013, Comput. Chem. Eng..

[28]  M. J. Kim,et al.  Mixing enhancement by biologically inspired convection in a micro-chamber using alternating current galvanotactic control of the Tetrahymena pyriformis , 2013 .

[29]  H. Morgan,et al.  Electrothermal flows generated by alternating and rotating electric fields in microsystems , 2006, Journal of Fluid Mechanics.

[30]  M. A. Ansari,et al.  A novel passive micromixer based on unbalanced splits and collisions of fluid streams , 2010 .

[31]  Yu Sanna Hui,et al.  A novel method to construct 3D electrodes at the sidewall of microfluidic channel , 2013 .

[32]  Jia-Kun Chen,et al.  Electroosmotic flow mixing in zigzag microchannels , 2007, Electrophoresis.

[33]  K. Nandakumar,et al.  Novel index for micromixing characterization and comparative analysis. , 2010, Biomicrofluidics.

[34]  Jing-Tang Yang,et al.  Analysis of chaos and FRET reaction in split-and-recombine microreactors , 2011 .

[35]  H. Morgan,et al.  Ac electrokinetics: a review of forces in microelectrode structures , 1998 .

[36]  Jianping Fu,et al.  Supplementary Information for Multiplex Serum Cytokine Immunoassay Using Nanoplasmonic Biosensor Microarrays , 2015 .

[37]  Y. Duan,et al.  Chemistry, biology, and medicine of fluorescent nanomaterials and related systems: new insights into biosensing, bioimaging, genomics, diagnostics, and therapy. , 2014, Chemical reviews.

[38]  P. Sheng,et al.  Characterizing and Patterning of PDMS‐Based Conducting Composites , 2007 .

[39]  Chang Liu,et al.  Micro magnetic stir-bar mixer integrated with parylene microfluidic channels. , 2004, Lab on a chip.

[40]  Anthony W Smith,et al.  Biofilms and antibiotic therapy: is there a role for combating bacterial resistance by the use of novel drug delivery systems? , 2005, Advanced drug delivery reviews.

[41]  O. Velev,et al.  Remotely powered distributed microfluidic pumps and mixers based on miniature diodes. , 2008, Lab on a chip.

[42]  G Medoro,et al.  Microfluidic channel fabrication in dry film resist for production and prototyping of hybrid chips. , 2005, Lab on a chip.

[43]  Yucheng Ding,et al.  A theoretical and numerical investigation of travelling wave induction microfluidic pumping in a temperature gradient , 2014 .

[44]  Hyoung J. Cho,et al.  Effect of laminar velocity profile variation on mixing in microfluidic devices: The sigma micromixer , 2008 .

[45]  Stuart J. Williams,et al.  Electrothermal pumping with interdigitated electrodes and resistive heaters , 2015, Electrophoresis.

[46]  J. Gimsa,et al.  A short review on AC electro-thermal micropumps based on smeared structural polarizations in the presence of a temperature gradient , 2011 .

[47]  I. Mezić,et al.  A theoretical and experimental study of ac electrothermal flows , 2012 .

[48]  Charles R. Doering,et al.  Stirring up trouble: Multi-scale mixing measures for steady scalar sources , 2007 .

[49]  Christina E. Dyllick,et al.  Analytical and Bioanalytical Chemistry , 2002 .

[50]  I. Nizameev,et al.  Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2015 .

[51]  Hongyuan Jiang,et al.  Continuous dielectrophoretic particle separation using a microfluidic device with 3D electrodes and vaulted obstacles , 2015, Electrophoresis.

[52]  Nadine Aubry,et al.  Electro-hydrodynamic micro-fluidic mixer. , 2003, Lab on a chip.