A Phylogenetic Analysis of Epilobium (Onagraceae) Based on Nuclear Ribosomal DNA Sequences

The internal transcribed spacers and 5.8S cistron of nuclear ribosomal DNA were sequenced from 22 species of Epilobium and two outgroups. Phylogenies were inferred from the sequences using parsimony, neighbor-joining, maximum-likelihood, and compatibility analysis. With parsimony we explored the effect of different weights for insertions/deletions (indels) vs. substitutions, coding vs. non-coding regions, transitions vs. transversions, and a posteriori character reweighting. Section Chamaenerion was found to be sister to the rest of the genus. The remainder of Epilobium fell into two main clades: 1) sect. Epilobium, and 2) the "xerophytic" clade, which comprises the remaining six sections. The unusual species E. rigidum (sect. Epilobium) had an uncertain position, appearing either at the base of sect. Epilobium, the base of the "xerophytic" clade, or as sister to the entire genus except sect. Chamaenerion. Within the "xerophytic" clade, relationships were incompletely resolved but a monophyletic group consisting of the annual sects. Boisduvalia and Currania (both formerly constituting the genus Boisduvalia) and the perennial, hummingbird- pollinated sect. Zauschneria was supported. The molecular phylogeny is compatible with some important independent characters and is useful for interpreting morphological and cytological evolution in Epilobium.

[1]  James F. Smith,et al.  A Qualitative Comparison of Total Cellular DNA Extraction Protocols , 1992 .

[2]  David G. Lloyd,et al.  Multi‐residue gaps, a class of molecular characters with exceptional reliability for phylogenetic analyses , 1991 .

[3]  T. White.  Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[4]  K. Bremer.  THE LIMITS OF AMINO ACID SEQUENCE DATA IN ANGIOSPERM PHYLOGENETIC RECONSTRUCTION , 1988, Evolution; international journal of organic evolution.

[5]  Ross A. Overbeek,et al.  The ribosomal database project , 1992, Nucleic Acids Res..

[6]  P. Raven,et al.  THE EVOLUTION OF POLLEN TETRADS IN ONAGRACEAE. , 1975, American journal of botany.

[7]  S. Carlquist.  Wood Anatomy of Onagraceae, with Notes on Alternative Modes of Photosynthate Movement in Dicotyledon Woods , 1975 .

[8]  J. Farris,et al.  Quantitative Phyletics and the Evolution of Anurans , 1969 .

[9]  Y. Heslop-Harrison.  Stigma form and surface in relation to self‐incompatibility in the Onagraceae , 1990 .

[10]  W. Maddison.  Missing Data versus Missing Characters in Phylogenetic Analysis , 1993 .

[11]  C. Bult,et al.  Nuclear Ribosomal RNA Sequences for Inferring Tribal Relationships Within Onagraceae , 1993 .

[12]  K. Sytsma,et al.  CHLOROPLAST DNA EVOLUTION AND PHYLOGENETIC RELATIONSHIPS IN CLARKIA SECT. PERIPETASMA (ONAGRACEAE) , 1986, Evolution; international journal of organic evolution.

[13]  J. Huelsenbeck.  Tree-Length Distribution Skewness: An Indicator of Phylogenetic Information , 1991 .

[14]  U. Gyllensten.  Direct Sequencing of In Vitro Amplified DNA , 1989 .

[15]  P. Hoch,et al.  A New Combination in Epilobium (Onagraceae) , 1979 .

[16]  R. Jorgensen,et al.  Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[17]  P. Raven,et al.  A COMPARATIVE STUDY OF MITOSIS IN THE ONAGRACEAE , 1962 .

[18]  J. Palmer,et al.  Nucleotide sequences of the rbcL gene indicate monophyly of mustard oil plants , 1993 .

[19]  Michael J. Sanderson,et al.  MONOPHYLY OF ANEUPLOID ASTRAGALUS (FABACEAE): EVIDENCE FROM NUCLEAR RIBOSOMAL DNA INTERNAL TRANSCRIBED SPACER SEQUENCES , 1993 .

[20]  G. Ledyard Stebbins,et al.  Chromosomal evolution in higher plants , 1971 .

[21]  K. Mullis,et al.  Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. , 1986, Cold Spring Harbor symposia on quantitative biology.

[22]  E. Zimmer,et al.  Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of Ribosomal DNA in Winteraceae , 1993 .

[23]  J. Huelsenbeck,et al.  Signal, noise, and reliability in molecular phylogenetic analyses. , 1992, The Journal of heredity.

[24]  James S. Farris,et al.  The retention index and homoplasy excess , 1989 .

[25]  S. R. Seavey.  Experimental Hybridization and Chromosome Homologies in Boisduvalia sect. Boisduvalia (Onagraceae) , 1992 .

[26]  E. Conti,et al.  Tribal relationships in Onagraceae: implications from rbcL sequence data , 1993 .

[27]  E. Pahlich,et al.  A rapid DNA isolation procedure for small quantities of fresh leaf tissue , 1980 .

[28]  H. Tobe,et al.  A Cladistic Analysis of the Plant Family Onagraceae , 1993 .

[29]  J A Lake,et al.  Evidence that eukaryotes and eocyte prokaryotes are immediate relatives. , 1992, Science.

[30]  Michael J Sanderson,et al.  CONFIDENCE LIMITS ON PHYLOGENIES: THE BOOTSTRAP REVISITED , 1989, Cladistics : the international journal of the Willi Hennig Society.

[31]  D. Soltis,et al.  MatK DNA Sequences and Phylogenetic Reconstruction in Saxifragaceae s. str , 1994 .

[32]  P. G. Martin,et al.  Phylogenetic Studies Using Protein Sequences Within the Order Myrtales , 1986 .

[33]  M. Donoghue,et al.  Phylogenetic relationships of Dipsacales based on rbcl sequences , 1992 .