In vitro evaluation of novel Zeolite-hydroxyapatite blended scaffold for dental tissue engineering

Main purpose of tissue engineering is creating appropriate conditions for the regeneration of tissues. Dental pulp-derived stem cells due to differentiation capacity and angiogenic properties have potential to regenerate dental pulp tissue. In the current experimental study poly caprolactone and poly L-lactic acid were synthesized by ring-opening polymerization method. The nano-hydroxyapatite and Zeolite were obtained by hydrothermal method. Morphological features and crystals properties of nHA and Zeolite were studied by X-ray diffraction. Nanofibers were fabricated using electrospinning method and investigated by FT-IR spectroscopy. DPSCs obtained from human source and proliferation and viability of them on electrospun scaffolds were evaluated by MTT assay. Also, the adhesion and proliferation of hDPSCs were investigated by SEM. The results showed that hDPSCs have the most viability and proliferation on the 1 st , 7 th , 14 th days on PCL-PLA/Zeolite scaffolds and maximum on the 3 rd day on PCL-PLA/nHA scaffolds. On the days of 7 th and 14 th , cell growth on scaffolds containing both nHA and Zeolite is better than sample that nHA is used alone with PCL-PLA. Briefly, by these results can be understand that Zeolite is a good agent in bone and tooth tissue engineering applications. More studies requires to investigate Zeolite effect on scaffold properties.

[1]  C. Park,et al.  A Review on Properties of Natural and Synthetic Based Electrospun Fibrous Materials for Bone Tissue Engineering , 2018, Membranes.

[2]  M. Kazanci,et al.  Observation of triple helix motif on electrospun collagen nanofibers and its effect on the physical and structural properties , 2018 .

[3]  Chwee Teck Lim,et al.  Nanofiber technology: current status and emerging developments , 2017 .

[4]  S. Saber-Samandari,et al.  Fabrication of hydroxyapatite-baghdadite nanocomposite scaffolds coated by PCL/Bioglass with polyurethane polymeric sponge technique , 2017 .

[5]  D. Kaigler,et al.  Dental Pulp Tissue Regeneration Using Dental Pulp Stem Cells Isolated and Expanded in Human Serum , 2017, Journal of endodontics.

[6]  A. Barzegar,et al.  Adhesion of mesenchymal stem cells to biomimetic polymers: A review. , 2017, Materials science & engineering. C, Materials for biological applications.

[7]  A. Akbarzadeh,et al.  The odontogenic differentiation of human dental pulp stem cells on hydroxyapatite-coated biodegradable nanofibrous scaffolds , 2016 .

[8]  K. Shakesheff,et al.  Dental pulp stem cells: function, isolation and applications in regenerative medicine , 2015, Journal of tissue engineering and regenerative medicine.

[9]  M. Saito,et al.  Tooth-derived stem cells: Update and perspectives. , 2015, World journal of stem cells.

[10]  H. B. Raghavendran,et al.  Microwave synthesis, characterization, bioactivity and in vitro biocompatibility of zeolite–hydroxyapatite (Zeo–HA) composite for bone tissue engineering applications , 2014 .

[11]  M. Rashidi,et al.  Bioengineering of Dental Pulp Stem Cells in a Microporous PNIPAAm-PLGA Scaffold , 2014 .

[12]  Sarang Sharma,et al.  Biomaterials in tooth tissue engineering: a review. , 2014, Journal of clinical and diagnostic research : JCDR.

[13]  Liang Yu,et al.  Preparation of zeolite-A/chitosan hybrid composites and their bioactivities and antimicrobial activities. , 2013, Materials science & engineering. C, Materials for biological applications.

[14]  T. Bein,et al.  Mesoporosity--a new dimension for zeolites. , 2013, Chemical Society reviews.

[15]  T. Xiao,et al.  Preparation, bioactivity, degradability and primary cell responses to an ordered mesoporous magnesium–calcium silicate , 2012 .

[16]  Shangtian Yang,et al.  Three-dimensional fibrous scaffolds with microstructures and nanotextures for tissue engineering , 2012 .

[17]  H. Ushijima,et al.  Bio-silica and bio-polyphosphate: applications in biomedicine (bone formation). , 2012, Current opinion in biotechnology.

[18]  Antonios G Mikos,et al.  Polymeric nanofibers in tissue engineering. , 2011, Tissue engineering. Part B, Reviews.

[19]  Jaebeom Lee,et al.  Nanoscale hydroxyapatite particles for bone tissue engineering. , 2011, Acta biomaterialia.

[20]  J. Nör,et al.  Pulp tissue from primary teeth: new source of stem cells , 2011, Journal of applied oral science : revista FOB.

[21]  K. Tanner.  Bioactive composites for bone tissue engineering , 2010, Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine.

[22]  Shicheng Wei,et al.  Electrospun PCL/PLA/HA based nanofibers as scaffold for osteoblast-like cells. , 2010, Journal of nanoscience and nanotechnology.

[23]  S. Vahabi,et al.  In vitro Growth and Characterization of Stem Cells from Human Dental Pulp of Deciduous Versus Permanent Teeth , 2010, Journal of dentistry.

[24]  M. Shokrgozar,et al.  Hydrothermal synthesis and characterization of hydroxyapatite and fluorhydroxyapatite nano-size powders , 2010, Biomedical materials.

[25]  María Vallet-Regí,et al.  Sol-gel silica-based biomaterials and bone tissue regeneration. , 2010, Acta biomaterialia.

[26]  S. Nair,et al.  Preparation and characterization of chitosan–gelatin/nanohydroxyapatite composite scaffolds for tissue engineering applications , 2010 .

[27]  Changyou Gao,et al.  Microscale control over collagen gradient on poly(L-lactide) membrane surface for manipulating chondrocyte distribution. , 2008, Colloids and surfaces. B, Biointerfaces.

[28]  H. Oudadesse,et al.  Surface and interface investigation of aluminosilicate biomaterial by the “in vivo” experiments , 2008 .

[29]  A. Stanishevsky,et al.  An electrospun triphasic nanofibrous scaffold for bone tissue engineering , 2007, Biomedical materials.

[30]  A. Bandyopadhyay,et al.  Calcium Phosphate‐Based Resorbable Ceramics: Influence of MgO, ZnO, and SiO2 Dopants , 2006 .

[31]  Laura A. Smith,et al.  Nano-fibrous scaffolds for tissue engineering. , 2004, Colloids and surfaces. B, Biointerfaces.

[32]  M Epple,et al.  A thorough physicochemical characterisation of 14 calcium phosphate-based bone substitution materials in comparison to natural bone. , 2004, Biomaterials.

[33]  A. Boccaccini,et al.  In vitro evaluation of novel bioactive composites based on Bioglass-filled polylactide foams for bone tissue engineering scaffolds. , 2003, Journal of biomedical materials research. Part A.

[34]  Dietmar W. Hutmacher,et al.  Scaffold design and fabrication technologies for engineering tissues — state of the art and future perspectives , 2001, Journal of biomaterials science. Polymer edition.

[35]  M. Rafienia,et al.  Fabrication and characterization of electrospun poly lactic-co-glycolic acid/zeolite nanocomposite scaffolds using bone tissue engineering , 2018 .

[36]  Tao Xu,et al.  Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation. , 2017, Biomaterials.

[37]  O. Baydik,et al.  [Tissue engineering in dentistry]. , 2015, Stomatologiia.

[38]  M. Vallet‐Regí,et al.  Calcium phosphates as substitution of bone tissues , 2004 .