Antibacterial and Anti-Biofilm Activities of Essential Oil Compounds against New Delhi Metallo-β-Lactamase-1-Producing Uropathogenic Klebsiella pneumoniae Strains

The World Health Organization points out that the opportunistic pathogen Klebsiella pneumoniae that causes various infections among others, urinary tract infections (UTIs), is one of the high-priority species due to a global problem of antimicrobial resistance. The aim of this study was to investigate antibacterial and anti-biofilm activities of chosen constituents of essential oils against NDM-1-producing, uropathogenic K. pneumoniae strains. The genes encoding lipopolysaccharide (uge, wabG), adhesin gene fimH (type I fimbriae) and gene encoding carbapenemase (blaNDM-1) for all tested strains were detected by PCR amplification. The K. pneumoniae ATCC BAA-2473 reference strain was uge- and blaNDM-1-positive. The effectiveness of fifteen essential oil compounds (EOCs) (linalool, β-citronellol, linalyl acetate, menthone, (−)-menthol, (+)-menthol, geraniol, eugenol, thymol, trans-anethole, farnesol, β-caryophyllene, (R)-(+)-limonene, 1,8-cineole, and carvacrol) was assessed by determining the MIC, MBC, MBC/MIC ratio against K. pneumoniae strains by the microdilution method. Anti-biofilm properties of these compounds were also investigated. Thymol, carvacrol and geraniol exhibited the best antibacterial and anti-biofilm activities against uropathogenic NDM-1-producing K. pneumoniae isolates. Results of our investigations provide a basis for more detailed studies of these phytochemicals on their application against uropathogenic K. pneumoniae.

[1]  J. Dzoyem,et al.  Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms , 2021, The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale.

[2]  Elaine Meade,et al.  Effective Antimicrobial Solutions for Eradicating Multi-Resistant and β-Lactamase-Producing Nosocomial Gram-Negative Pathogens , 2021, Antibiotics.

[3]  Giovanni Parente,et al.  Pyelonephritis in Pediatric Uropathic Patients: Differences from Community-Acquired Ones and Therapeutic Protocol Considerations. A 10-Year Single-Center Retrospective Study , 2021, Children.

[4]  Caio H. N. Barros,et al.  Synthesis and self-assembly of curcumin-modified amphiphilic polymeric micelles with antibacterial activity , 2021, Journal of Nanobiotechnology.

[5]  R. Ranjbar,et al.  Virulence factors, antibiotic resistance patterns, and molecular types of clinical isolates of Klebsiella Pneumoniae , 2021, Expert review of anti-infective therapy.

[6]  A. van Belkum,et al.  Global genotype distribution of human clinical isolates of New Delhi metallo-β-lactamase-producing Klebsiella pneumoniae; A systematic review. , 2020, Journal of global antimicrobial resistance.

[7]  Z. Suntres,et al.  The antibacterial properties of phenolic isomers, carvacrol and thymol , 2020, Critical reviews in food science and nutrition.

[8]  G. Rajivgandhi,et al.  Preparative HPLC fraction of Hibiscus rosa-sinensis essential oil against biofilm forming Klebsiella pneumoniae , 2020, Saudi journal of biological sciences.

[9]  H. Greige‐Gerges,et al.  Recent advances in tackling microbial multidrug resistance with essential oils: combinatorial and nano-based strategies , 2020, Critical reviews in microbiology.

[10]  F. O. Pereira,et al.  Antimicrobial activity of geraniol: an integrative review , 2020 .

[11]  E. Tyczkowska-Sieroń,et al.  Etiology and bacterial susceptibility to antibiotics in patients with recurrent lower urinary tract infections. , 2020, Polish archives of internal medicine.

[12]  C. Wiart,et al.  Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq. against bacterial clinical isolates , 2020, BMC complementary medicine and therapies.

[13]  S. Hamam,et al.  Study of Various Virulence Genes, Biofilm Formation and Extended-Spectrum β-lactamase Resistance in Klebsiella pneumoniae Isolated from Urinary Tract Infections , 2019, The Open Microbiology Journal.

[14]  T. Petkovits,et al.  Anti-Biofilm Effect of Selected Essential Oils and Main Components on Mono- and Polymicrobic Bacterial Cultures , 2019, Microorganisms.

[15]  P. Kwiatkowski,et al.  The Influence of Essential Oil Compounds on Antibacterial Activity of Mupirocin-Susceptible and Induced Low-Level Mupirocin-Resistant MRSA Strains , 2019, Molecules.

[16]  S. Giedrys-Kalemba,et al.  Preliminary Study on the Antibacterial Activity of Essential Oils Alone and in Combination with Gentamicin Against Extended-Spectrum β-Lactamase-Producing and New Delhi Metallo-β-Lactamase-1-Producing Klebsiella pneumoniae Isolates. , 2018, Microbial drug resistance.

[17]  Wei Wang,et al.  Antibiotic resistance: a rundown of a global crisis , 2018, Infection and drug resistance.

[18]  F. Codjoe,et al.  Carbapenem Resistance: A Review , 2017, Medical sciences.

[19]  M. Maffei,et al.  UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies , 2017, Front. Microbiol..

[20]  N. Nahar,et al.  Phylogenetic Analysis of Antibiotic Resistance Genes and Virulence Genes of Klebsiella species in silico , 2017 .

[21]  H. Kafil,et al.  Thymol and carvacrol strongly inhibit biofilm formation and growth of carbapenemase-producing Gram negative bacilli. , 2017, Cellular and molecular biology.

[22]  Wissem Mnif,et al.  Essential Oils’ Chemical Characterization and Investigation of Some Biological Activities: A Critical Review , 2016, Medicines.

[23]  J. Mecsas,et al.  Klebsiella pneumoniae: Going on the Offense with a Strong Defense , 2016, Microbiology and Molecular Reviews.

[24]  N. Aksöz,et al.  Klebsiella pneumoniae: characteristics of carbapenem resistance and virulence factors. , 2015, Acta biochimica Polonica.

[25]  A. Kimbaris,et al.  Antimicrobial activity of essential oils of cultivated oregano (Origanum vulgare), sage (Salvia officinalis), and thyme (Thymus vulgaris) against clinical isolates of Escherichia coli, Klebsiella oxytoca, and Klebsiella pneumoniae , 2015, Microbial ecology in health and disease.

[26]  K. Kaye,et al.  Treatment Options for Carbapenem-Resistant Enterobacteriaceae Infections , 2015, Open forum infectious diseases.

[27]  J. Stachowiak,et al.  The role of the healthcare environment in the spread of multidrug-resistant organisms: update on current best practices for containment , 2014, Therapeutic advances in infectious disease.

[28]  R. Coppola,et al.  Effect of Essential Oils on Pathogenic Bacteria , 2013, Pharmaceuticals.

[29]  K. Ribbeck,et al.  Selected Antimicrobial Essential Oils Eradicate Pseudomonas spp. and Staphylococcus aureus Biofilms , 2012, Applied and Environmental Microbiology.

[30]  G. Arlet,et al.  Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. , 2010, The Journal of antimicrobial chemotherapy.

[31]  R. Tournebize,et al.  Virulent Clones of Klebsiella pneumoniae: Identification and Evolutionary Scenario Based on Genomic and Phenotypic Characterization , 2009, PloS one.

[32]  J. Pinkner,et al.  Molecular Variations in Klebsiella pneumoniae and Escherichia coli FimH Affect Function and Pathogenesis in the Urinary Tract , 2008, Infection and Immunity.

[33]  Y. Carmeli,et al.  Isolation of Imipenem-Resistant Enterobacter Species: Emergence of KPC-2 Carbapenemase, Molecular Characterization, Epidemiology, and Outcomes , 2008, Antimicrobial Agents and Chemotherapy.

[34]  Ruurd van der Zee,et al.  Carvacrol Induces Heat Shock Protein 60 and Inhibits Synthesis of Flagellin in Escherichia coli O157:H7 , 2007, Applied and Environmental Microbiology.

[35]  C. Shun,et al.  Genomic heterogeneity in Klebsiella pneumoniae strains is associated with primary pyogenic liver abscess and metastatic infection. , 2005, The Journal of infectious diseases.

[36]  Chia-Tung Shun,et al.  A Novel Virulence Gene in Klebsiella pneumoniae Strains Causing Primary Liver Abscess and Septic Metastatic Complications , 2004, The Journal of experimental medicine.

[37]  M. Regué,et al.  The Klebsiella pneumoniae wabG Gene: Role inBiosynthesis of the Core Lipopolysaccharide andVirulence , 2003, Journal of bacteriology.

[38]  M. Ferraro.  Performance standards for antimicrobial susceptibility testing , 2001 .