The Influence of Ossification Ability of the Mesenchymal Stem Cells co-cultured with Vascular Endothelial Cell in Vitro.

Objective:To evaluate the effect of endothelial cell to marrow mesenchymal stem cell in in three-dimensional system.Methods:dissociation and culture rat bone marrow mesenchymal stem cell and endothelial cell,and in different co-culture models(co-culture with or without direct contact), Analysis ossification ability of bone marrow mesenchymal stem cell.Results:In directive and indirective co-culture and marrow mesenchymal stem cell alone culture ,Protein quantity is (0.239±0.023) mg/mL,(0.174±0.015) mg/mL,(0.115±0.012) mg/mL,(0.121±0.016) mg/mL; The ALP activity are (0.625±0.123) μ/mg、(0.412±0.178) μ/mg、(0.141±0.08) μ/mg;Osteocalcin are (0.8±0.124) μg/L、(0.462±0.149) μg/L、(0.235±0.146) μg/L.By statistics evaluation, To ossification ability of bone marrow mesenchymal stem cell co-culture is higher than culture alone(P0.05),and direct is higher than indirect(P0.05).Conclusions:Ossification ability of bone marrow mesenchymal stem cell can be regulated in co-culture with Endothelial cell by cytokine and cell membrane proteins.