Nanofibrous Membranes Containing Carbon Nanotubes: Electrospun for Redox Enzyme Immobilization

Summary: Nanofibrous membranes that possess reactivegroups are fabricated by the electrospinning process fromPANCAA solutions that contain MWCNTs. Field emissionscanning electron microscopy is used to evaluate themorphology and diameter of the nanofibers. Potentials forapplying these nanofibrous membranes to immobilize redoxenzymes by covalent bonding are explored. It is envisagedthat the electrospun nanofibrous membranes could provide alarge specific area and the MWCNTs could donate/acceptelectrons for the immobilized redox enzymes. Resultsindicate that, after blending with MWCNTs, the diameterofthePANCAAnanofiberincreasesslightly.ThePANCAA/MWCNT nanofibrous membranes immobilize moreenzymes than that without MWCNTs. Moreover, as theconcentration of the MWCNTs increases, the activity ofthe immobilized catalase is enhanced by about 42%, whichis mainly attributed to the promoted electron transferthrough charge-transfer complexes and the p system ofMWCNTs.The covalent immobilization of redox enzymes, such ascatalase, on a PANCAA/MWCNTs nanofiber.

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