Biological processing in oscillatory baffled reactors: operation, advantages and potential

The development of efficient and commercially viable bioprocesses is essential for reducing the need for fossil-derived products. Increasingly, pharmaceuticals, fuel, health products and precursor compounds for plastics are being synthesized using bioprocessing routes as opposed to more traditional chemical technologies. Production vessels or reactors are required for synthesis of crude product before downstream processing for extraction and purification. Reactors are operated either in discrete batches or, preferably, continuously in order to reduce waste, cost and energy. This review describes the oscillatory baffled reactor (OBR), which, generally, has a niche application in performing ‘long’ processes in plug flow conditions, and so should be suitable for various bioprocesses. We report findings to suggest that OBRs could increase reaction rates for specific bioprocesses owing to low shear, good global mixing and enhanced mass transfer compared with conventional reactors. By maintaining geometrical and dynamic conditions, the technology has been proved to be easily scaled up and operated continuously, allowing laboratory-scale results to be easily transferred to industrial-sized processes. This is the first comprehensive review of bioprocessing using OBRs. The barriers facing industrial adoption of the technology are discussed alongside some suggested strategies to overcome these barriers. OBR technology could prove to be a major aid in the development of commercially viable and sustainable bioprocesses, essential for moving towards a greener future.

[1]  Chein‐Chi Chang,et al.  Anaerobic Process , 2015, Water environment research : a research publication of the Water Environment Federation.

[2]  K. Plumb,et al.  Continuous Processing in the Pharmaceutical Industry: Changing the Mind Set , 2005 .

[3]  Dimitri Mignard,et al.  Determination of breakage rates of oil droplets in a continuous oscillatory baffled tube , 2006 .

[4]  S. Nagata.  Mixing: Principles and Applications , 1975 .

[5]  Xiongwei Ni.  Residence time distribution measurements in a pulsed baffled tube bundle , 1994 .

[6]  S. Mazer,et al.  Plant ecotypes: genetic differentiation in the age of ecological restoration , 2003 .

[7]  D. Budman,et al.  Trastuzumab-DM1: A Review of the Novel Immuno-Conjugate for HER2- Overexpressing Breast Cancer , 2009 .

[8]  Larry A. Glasgow,et al.  Characterization of Agitation Intensity in Flocculation Processes , 1986 .

[9]  David C. Sherrington,et al.  Butylation of phenylacetonitrile in an oscillatory baffled reactor , 2005 .

[10]  M. Demerdash,et al.  Thermal deactivation kinetics of CM-cellulase from a local isolate of Aspergillus niger (RD-2231). , 1992, Zentralblatt fur Mikrobiologie.

[11]  Jyeshtharaj B. Joshi,et al.  Cellulase deactivation in a stirred reactor , 2000 .

[12]  Cameron J. Brown,et al.  Evaluation of growth kinetics of antisolvent crystallization of paracetamol in an oscillatory baffled crystallizer utilizing video imaging , 2011 .

[13]  R. Skelton,et al.  The application of oscillatory flow mixing to photocatalytic wet oxidation , 1999 .

[14]  Anh N. Phan,et al.  Development and evaluation of novel designs of continuous mesoscale oscillatory baffled reactors , 2010 .

[15]  P. Whittington,et al.  The use of laminar tube flow in the study of hydrodynamic and chemical influences on polymer flocculation of Escherichia coli , 1992, Biotechnology and bioengineering.

[16]  R. Seviour,et al.  Influence of bioreactor design on exopolysaccharide production byAureobasidium pullulans , 1992, Biotechnology Letters.

[17]  M Kumakura.  Effect of calcium ions on the irradiation induced inactivation of cellulase. , 1996, Isotopes in environmental and health studies.

[18]  I. Sobey.  On flow through furrowed channels. Part 1. Calculated flow patterns , 1980, Journal of Fluid Mechanics.

[19]  D. A. Deglon,et al.  Flotation in a novel oscillatory baffled column , 2009 .

[20]  B. Bellhouse,et al.  On flow through furrowed channels. Part 2. Observed flow patterns , 1980 .

[21]  Malcolm R. Mackley,et al.  Experimental observations on flow patterns and energy losses for oscillatory flow in ducts containing sharp edges , 1989 .

[22]  Claudia N Troeger,et al.  The Production of Polyhydroxyalkanoates Using an Oscillatory Baffled Bioreactor , 2009 .

[23]  Xiongwei Ni,et al.  A comparative study of mass transfer in yeast for a batch pulsed baffled bioreactor and a stirred tank fermenter , 1995 .

[24]  Lee R. Lynd,et al.  A Product‐Nonspecific Framework for Evaluating the Potential of Biomass‐Based Products to Displace Fossil Fuels , 2003 .

[25]  Anh N. Phan,et al.  Effect of geometrical parameters on fluid mixing in novel mesoscale oscillatory helical baffled designs , 2011 .

[26]  Gerry Steele,et al.  Continuous Crystallization of Pharmaceuticals Using a Continuous Oscillatory Baffled Crystallizer , 2009 .

[27]  B. McNeil,et al.  Influence of impeller speed upon the pullulan fermentation , 1987, Biotechnology Letters.

[28]  P.T.L. Koh,et al.  CFD modelling of bubble–particle collision rates and efficiencies in a flotation cell , 2003 .

[29]  J. Ikwebe.  Intensification of bioethanol production by simultaneous saccharification and fermentation in an oscillatory baffled reactor , 2011 .

[30]  Andrej Bombač,et al.  Individual impeller flooding in aerated vessel stirred by multiple-Rushton impellers , 2006 .

[31]  Keith Buchanan Smith.  Scale-up of oscillatory flow mixing , 2000 .

[32]  J Tramper,et al.  Shear sensitivity of animal cells from a culture-medium perspective. , 1998, Trends in biotechnology.

[33]  Lian Pin Koh,et al.  The biofuel potential of municipal solid waste , 2009 .

[34]  Nibedita Sarkar,et al.  Bioethanol production from agricultural wastes: An overview , 2012 .

[35]  Adam Harvey,et al.  A Mixing-Based Design Methodology for Continuous Oscillatory Flow Reactors , 2002 .

[36]  Abdul Wahab Mohammad,et al.  Solvent fermentation from palm oil mill effluent using clostridium acetobutylicum in oscillatory flow bioreactor , 2009 .

[37]  Xiongwei Ni,et al.  Gas hold-up and bubble diameters in a gassed oscillatory baffled column , 2001 .

[38]  Anh N. Phan,et al.  Continuous screening of base-catalysed biodiesel production using New designs of mesoscale oscillato , 2011 .

[39]  António A. Vicente,et al.  The intensification of gas–liquid flows with a periodic, constricted oscillatory-meso tube , 2007 .

[40]  Miguel Olaizola,et al.  Haematococcus astaxanthin: applications for human health and nutrition. , 2003, Trends in biotechnology.

[41]  James J. De Yoreo,et al.  Crystallization of Paracetamol under Oscillatory Flow Mixing Conditions , 2004 .

[42]  E. Reese,et al.  Shear inactivation of cellulase of Trichoderma reesei , 1980 .

[43]  Nuno M. Reis,et al.  Novel oscillatory flow reactors for biotechnological applications , 2006 .

[44]  J. Tramper,et al.  Shear sensitivity of insect cells in suspension , 1986 .

[45]  Malcolm R. Mackley,et al.  AN EXPERIMENTAL INVESTIGATION INTO THE SCALE-UP OF OSCILLATORY FLOW MIXING IN BAFFLED TUBES , 2006 .

[46]  Y. Chisti,et al.  Hydrodynamic Damage to Animal Cells , 2001, Critical reviews in biotechnology.

[47]  Malcolm R. Mackley,et al.  Heat transfer and associated energy dissipation for oscillatory flow in baffled tubes , 1995 .

[48]  O. Levenspiel.  Chemical Reaction Engineering , 1972 .

[49]  A. Mulchandani,et al.  Oxygen requirement in pullulan fermentation , 1988, Applied Microbiology and Biotechnology.

[50]  Aniruddha B. Pandit,et al.  Enhancement of gas-liquid mass transfer using oscillatory flow in a baffled tube , 1993 .

[51]  G. Rossi.  The design of bioreactors , 1999 .

[52]  M. F. Edwards,et al.  Mixing in the process industries , 1985 .

[53]  Brian McNeil,et al.  Fermentation of Pullulan Using an Oscillatory Baffled Fermenter , 2005 .

[54]  Anh N. Phan,et al.  Characterisation of fluid mixing in novel designs of mesoscale oscillatory baffled reactors operating at low flow rates (0.3–0.6 ml/min) , 2011 .

[55]  Philippe A. Tanguy,et al.  A new investigation of the metzner‐otto concept for anchor mixing impellers , 1996 .

[56]  Chih-Sheng Lin,et al.  Reduction of CO2 by a high-density culture of Chlorella sp. in a semicontinuous photobioreactor. , 2008, Bioresource technology.

[57]  Hongbing Jian,et al.  A Numerical Study on the Scale-Up Behaviour in Oscillatory Baffled Columns , 2005 .

[58]  Adam Harvey,et al.  Potential uses of oscillatory baffled reactors for biofuel production , 2010 .

[59]  Kevin J. Roberts,et al.  On the Crystal Polymorphic Forms of l-Glutamic Acid Following Temperature Programmed Crystallization in a Batch Oscillatory Baffled Crystallizer , 2004 .

[60]  Xiongwei Ni,et al.  On the discussion of the dimensionless groups governing oscillatory flow in a baffled tube , 1997 .

[61]  C. Jungo,et al.  An Emerging Star for Therapeutic and Catalytic Protein Production , 2008 .

[62]  P. Stonestreet,et al.  Protein refolding in an oscillatory flow reactor , 2004, Biotechnology Letters.

[63]  Dimitri Mignard,et al.  Modelling of droplet breakage probabilities in an oscillatory baffled reactor , 2004 .

[64]  Daniel Chaumont,et al.  Cell fragility — The key problem of microalgae mass production in closed photobioreactors , 1991 .

[65]  Anton P. J. Middelberg,et al.  The influence of mixing on lysozyme renaturation during refolding in an oscillatory flow and a stirred-tank reactor , 2002 .

[66]  S. Allen,et al.  Kinetic dynamics in heterogeneous enzymatic hydrolysis of cellulose: an overview, an experimental study and mathematical modelling , 2003 .

[67]  Malcolm R. Mackley,et al.  A pulsatile flow bioreactor , 1992 .

[68]  John A. Heitmann,et al.  Deactivation of cellulase and hemicellulase in high shear fields , 1996 .

[69]  Xiongwei Ni,et al.  A study of oil-water dispersion in a pulsed baffled reactor , 1996 .

[70]  A. Lübbert,et al.  Bioreactor Retrofitting to Avoid Aeration with Oxygen in Pichia Pastoris Cultivation Processes for Recombinant Protein Production , 2004 .

[71]  John N. Saddler,et al.  Effects of sugar inhibition on cellulases and β-glucosidase during enzymatic hydrolysis of softwood substrates , 2004 .

[72]  Y. Tashiro,et al.  Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: overview and limits. , 2011, Journal of biotechnology.

[73]  Jos Derksen,et al.  Turbulent mixing in a tubular reactor: Assessment of an FDF/LES approach , 2005 .

[74]  Xiongwei Ni,et al.  Correlation of polymer particle size with droplet size in suspension polymerisation of methylmethacrylate in a batch oscillatory-baffled reactor , 1999 .

[75]  D. Gregg,et al.  Effects of sugar inhibition on cellulases and beta-glucosidase during enzymatic hydrolysis of softwood substrates. , 2004, Applied biochemistry and biotechnology.

[76]  P. Stonestreet,et al.  The Effects of Oscillatory Flow and Bulk Flow Components on Residence Time Distribution in Baffled Tube Reactors , 1999 .

[77]  Clive A. Greated,et al.  Experimental Study of Flocculation of Bentonite and Alcaligenes Eutrophus in a Batch Oscillatory Baffled Flocculator , 2001 .

[78]  J. Teixeira,et al.  Proof‐of‐concept of a novel micro‐bioreactor for fast development of industrial bioprocesses , 2006, Biotechnology and bioengineering.

[79]  Adeniyi Lawal,et al.  Continuous plug-flow bioreactor: experimental testing with Pseudomonas putida culture grown on benzoate. , 2005, Biotechnology and bioengineering.

[80]  U. Onken,et al.  Influence of dissolved oxygen concentration and shear rate on the production of pullulan byAureobasidium pullulans , 1991, Biotechnology Letters.

[81]  R. Jaenicke,et al.  A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme. , 1991, Biochemistry.

[82]  Ping Gao,et al.  Photooxidation of a model pollutant in an oscillatory flow reactor with baffles , 2003 .

[83]  A. A. Vicente,et al.  Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium , 2006, Biotechnology Letters.

[84]  X. Ni.  Continuous oscillatory baffled reactor technology , 2006 .

[85]  Xiongwei Ni,et al.  A Systematic Study of the Effect of Geometrical Parameters on Mixing Time in Oscillatory Baffled Columns , 1998 .

[86]  John Villadsen,et al.  Bioreactors : a chemical engineering perspective , 2001 .

[87]  R. I. Ristic,et al.  Crystallization by Oscillatory and Conventional Mixing at Constant Power Density (R&D Note) , 2005 .

[88]  S. B. Sawant,et al.  Shear Deactivation of Cellulase, Exoglucanase, Endoglucanase, and β‐Glucosidase in a Mechanically Agitated Reactor , 2001, Biotechnology progress.

[89]  B. Junker.  Scale-up methodologies for Escherichia coli and yeast fermentation processes. , 2004, Journal of bioscience and bioengineering.

[90]  Clive A. Greated,et al.  On the measurement of strain rate in an oscillatory baffled column using particle image velocimetry , 2000 .

[91]  Adam Harvey,et al.  Operation and Optimization of an Oscillatory Flow Continuous Reactor , 2001 .

[92]  R. I. Ristic,et al.  Oscillatory Mixing for Crystallization of High Crystal Perfection Pharmaceuticals , 2007 .

[93]  F. García-Ochoa,et al.  Bioreactor scale-up and oxygen transfer rate in microbial processes: an overview. , 2009, Biotechnology advances.

[94]  Cameron J. Brown,et al.  Online evaluation of paracetamol antisolvent crystallization growth rate with video imaging in an oscillatory baffled crystallizer , 2011 .

[95]  U. Rau,et al.  Enhanced glucan formation of filamentous fungi by effective mixing, oxygen limitation and fed-batch processing , 2005, Journal of Industrial Microbiology.

[96]  Adam Harvey,et al.  Process intensification of biodiesel production using a continuous oscillatory flow reactor , 2003 .

[97]  Malcolm R. Mackley,et al.  Experimental residence time distribution measurements for unsteady flow in baffled tubes , 1989 .

[98]  Anh N. Phan,et al.  Characterisation of mesoscale oscillatory helical baffled reactor—Experimental approach , 2012 .