Effect of Disulfiram (Tetrnethylthiurnin Disulphide) on the Metabolism of Methanol in Rat Liver Homogenates

. The effect of disulfiram on the accumulation of formaldehyde in liver homogenates incubated with methanol has been studied in rats after feeding them high doses of disulfiram (1.0–4.5 g per kg daily for 9 days). The catalase and xanthine oxidase activities of the livers have also been determined. The formation of formaldehyde has been determined photometrically. The accumulation of formaldehyde in the liver homogenates from disulfiram-treated rats was not increased, but slightly inhibited by almost lethal doses. It was not stimulated by ATP as in normal animals. The liver catalase activity was clearly lowered in the disulfiram-treated rats. The liver xanthine oxidase activity was also diminished in these rats but not constantly.

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